ZMIZ2 (NM_001300959) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	hZIMP7; NET27; TRAFIP20; ZIMP7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239702 representing NM_001300959 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCATGAACCCCATGAAACCTGCCCTGCCCCCTGCGCCACACGGTGATGGTTCATTCGCATATG AGTCTGTGCCTTGGCAACAAAGCGCCACTCAGCCGGCTGGATCGCTGTCTGTGGTCACTACTGTGTGGGG AGTTGGCAACGCGACACAGAGCCAGTGCCTGGGACAGCAGGCGTTTGCTGAAGGCGGCGCCAACAAGGGC TACGTGCAGCAAGGCGTGTACAGCCGCGGGGGCTACCCTGGGGCCCCCGGCTTCACCACCGGGTATGCAG GCGGCCCGGGGGGCCTGGGCCTCCCCTCACATGCTGCAAGACCCTCCACTGACTTCACGCAAGCGGCAGC TGCTGCAGCTGTGGCTGCTGCGGCAGCCACTGCCACCGCCACAGCCACAGCCACCGTGGCTGCTCTCCAG GAGAAGCAGAGCCAGGAGCTGAGCCAGTATGGAGCGATGGGGGCCGGACAGTCTTTTAACAGCCAGTTTC TGCAGCATGGAGGTCCCCGGGGGCCTAGTGTCCCCGCTGGCATGAACCCTACTGGCATAGGAGGGGTAAT GGGCCCCTCTGGCCTCTCCCCCTTGGCTATGAACCCCACCCGGGCAGCAGGAATGACACCCTTGTATGCA GGGCAGCGTCTGCCCCAACATGGGTATCCTGGGCCTCCCCAGGCCCAGCCACTGCCCCGACAGGGGGTCA AGAGAACCTACTCTGAGGTGTATCCAGGGCAGCAGTATCTGCAAGGAGGCCAGTATGCACCCAGCACCGC CCAGTTTGCGCCCAGCCCTGGGCAGCCCCCTGCCCCCTCCCCTTCCTACCCTGGGCACAGGCTGCCCCTG CAGCAGGGCATGACCCAGTCCCTGTCCGTGCCTGGCCCCACGGGACTGCATTATAAGCCTACCCGTTCCA TCCCGGGCTATCCCAGTTCCCCACTGCCAGGGAACCCCACGCCACCCATGACCCCAAGCAGCAGCGTCCC TTACATGTCACCAAACCAAGAGGTCAAGTCTCCCTTCTTGCCTGATCTCAAGCCCAACCTCAACTCCTTG CACTCATCGCCCTCTGGAAGCGGGCCTTGTGACGAGTTGCGGCTGACCTTCCCTGTGCGCGATGGGGTGG TCCTGGAGCCCTTCCGCCTGCAGCACAACCTGGCTGTAAGCAACCATGTCTTCCAGCTGCGAGACTCAGT CTACAAGACCCTGATAATGAGGCCTGACCTGGAGCTGCAATTCAAGTGCTACCACCACGAGGACCGGCAG ATGAACACCAACTGGCCAGCCTCGGTGCAGGTCAGCGTCAATGCCACGCCGCTCACCATCGAGCGTGGCG ACAACAAGACCTCGCACAAGCCACTCTACCTGAAGCATGTGTGCCAGCCAGGCCGCAACACCATCCAGAT CACCGTCACCGCCTGCTGCTGCTCCCACCTCTTCGTGCTGCAGCTAGTGCACCGCCCATCCGTCCGCTCG GTGCTGCAGGGCCTCCTCAAAAAGCGCCTCCTGCCTGCTGAGCACTGCATCACCAAGATAAAGCGGAACT TCAGCAGCGGCACCATCCCTGGCACCCCTGGGCCCAACGGAGAGGACGGGGTGGAGCAGACAGCTATCAA GGTGTCCCTGAAGTGCCCCATCACCTTCCGCAGGATCCAGCTCCCTGCCCGAGGTCATGACTGTCGCCAC ATACAGTGCTTTGACCTGGAGTCGTACCTGCAGCTCAACTGTGAGCGGGGGACTTGGAGGTGTCCTGTGT GCAACAAGACAGCTTTGCTGGAGGGCCTGGAGGTGGACCAGTACATGCTGGGCATCCTGATTTACATTCA GAACTCTGACTATGAGGAGATCACCATCGACCCCACGTGCAGCTGGAAGCCAGTGCCCGTGAAGCCTGAC ATGCACATCAAGGAGGAGCCGGATGGGCCAGCACTGAAGCGCTGCCGCACCGTGAGCCCCGCCCACGTGC TCATGCCCAGCGTGATGGAGATGATCGCCGCCCTGGGCCCCGGCGCTGCCCCCTTTGCCCCCCTGCAGCC CCCCTCAGTCCCTGCCCCCAGCGACTACCCTGGCCAGGGTTCCAGCTTCCTGGGGCCTGGAACTTTCCCT GAGTCCTTCCCACCCACCACGCCCAGCACCCCAACCCTTGCTGAGTTCACCCCGGGACCACCCCCCATCT CCTACCAGTCTGACATTCCCAGCAGCCTCCTGACTTCAGAGAAGTCTACCGCCTGCCTCCCAAGCCAGAT GGCACCAGCAGGTCACCTGGACCCCACTCACAATCCTGGGACACCAGGACTACACACCTCCAACCTTGGG GCCCCTCCAGGTCCCCAGCTGCACCATTCAAACCCTCCCCCAGCGTCCCGGCAGTCCTTGGGCCAAGCGA GCTTAGGACCTACGGGTGAACTGGCCTTCAGTCCTGCCACAGGCGTGATGGGGCCCCCCAGCATGTCTGG AGCCGGGGAGGCCCCAGAACCAGCTCTGGACCTGCTCCCGGAACTGACCAACCCTGATGAGCTACTGTCC TACTTGGGCCCACCCGACCTCCCTACGAACAACAATGACGACCTGCTTTCTCTGTTTGAGAACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239702 representing NM_001300959 Red=Cloning site Green=Tags(s) MNSMNPMKPALPPAPHGDGSFAYESVPWQQSATQPAGSLSVVTTVWGVGNATQSQCLGQQAFAEGGANKG YVQQGVYSRGGYPGAPGFTTGYAGGPGGLGLPSHAARPSTDFTQAAAAAAVAAAAATATATATATVAALQ EKQSQELSQYGAMGAGQSFNSQFLQHGGPRGPSVPAGMNPTGIGGVMGPSGLSPLAMNPTRAAGMTPLYA GQRLPQHGYPGPPQAQPLPRQGVKRTYSEVYPGQQYLQGGQYAPSTAQFAPSPGQPPAPSPSYPGHRLPL QQGMTQSLSVPGPTGLHYKPTRSIPGYPSSPLPGNPTPPMTPSSSVPYMSPNQEVKSPFLPDLKPNLNSL HSSPSGSGPCDELRLTFPVRDGVVLEPFRLQHNLAVSNHVFQLRDSVYKTLIMRPDLELQFKCYHHEDRQ MNTNWPASVQVSVNATPLTIERGDNKTSHKPLYLKHVCQPGRNTIQITVTACCCSHLFVLQLVHRPSVRS VLQGLLKKRLLPAEHCITKIKRNFSSGTIPGTPGPNGEDGVEQTAIKVSLKCPITFRRIQLPARGHDCRH IQCFDLESYLQLNCERGTWRCPVCNKTALLEGLEVDQYMLGILIYIQNSDYEEITIDPTCSWKPVPVKPD MHIKEEPDGPALKRCRTVSPAHVLMPSVMEMIAALGPGAAPFAPLQPPSVPAPSDYPGQGSSFLGPGTFP ESFPPTTPSTPTLAEFTPGPPPISYQSDIPSSLLTSEKSTACLPSQMAPAGHLDPTHNPGTPGLHTSNLG APPGPQLHHSNPPPASRQSLGQASLGPTGELAFSPATGVMGPPSMSGAGEAPEPALDLLPELTNPDELLS YLGPPDLPTNNNDDLLSLFENN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300959
ORF Size	2586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300959.1, NP_001287888.1
RefSeq Size	4983 bp
RefSeq ORF	2589 bp
Locus ID	83637
UniProt ID	Q8NF64
MW	91.6 kDa
Gene Summary	ZMIZ2 and ZMIZ1 (MIM 607159) are members of a PIAS (see MIM 603566)-like family of proteins that interact with nuclear hormone receptors. ZMIZ2 interacts with androgen receptor (AR; MIM 313700) and enhances AR-mediated transcription (Huang et al., 2005 [PubMed 16051670]).[supplied by OMIM, May 2010]

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