TACC2 (NM_001291878) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AZU-1; ECTACC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239878 representing NM_001291878 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGGTCGCAGTCCCTGCAGCCAGCCCCAGCCAGCGACCTGAACCTGGAGGCTTCCGAGGCAATGA GTTCCGATTCTGAAGAGGCATTTGAGACCCCGGAGTCAACGACCCCTGTCAAAGCTCCGCCAGCTCCACC CCCACCACCCCCCGAAGTCATCCCAGAACCCGAGGTCAGCACACAGCCACCCCCGGAAGAACCAGGATGT GGTTCTGAGACAGTCCCTGTCCCTGATGGCCCACGGAGCGACTCGGTGGAAGGAAGTCCCTTCCGTCCCC CGTCACACTCCTTCTCTGCCGTCTTCGATGAAGACAAGCCGATAGCCAGCAGTGGGACTTACAACTTGGA CTTTGACAACATTGAGCTTGTGGATACCTTTCAGACCTTGGAGCCTCGTGCCTCAGACGCTAAGAATCAG GAGGGCAAAGTGAACACACGGAGGAAGTCCACGGATTCCGTCCCCATCTCTAAGTCTACACTGTCCCGGT CGCTCAGCCTGCAAGCCAGTGACTTTGATGGTGCTTCTTCCTCAGGCAATCCCGAGGCCGTGGCCCTTGC CCCAGATGCATATAGCACGGGTTCCAGCAGTGCTTCTAGTACCCTTAAGCGAACTAAAAAACCGAGGCCG CCTTCCTTAAAAAAGAAACAGACCACCAAGAAACCCACAGAGACCCCCCCAGTGAAGGAGACGCAACAGG AGCCAGATGAAGAGAGCCTTGTCCCCAGTGGGGAGAATCTAGCATCTGAGACGAAAACGGAATCTGCCAA GACGGAAGGTCCTAGCCCAGCCTTATTGGAGGAGACGCCCCTTGAGCCCGCTGTGGGGCCCAAAGCTGCC TGCCCTCTGGACTCAGAGAGTGCAGAAGGGGTTGTCCCCCCGGCTTCTGGAGGTGGCAGAGTGCAGAACT CACCCCCTGTCGGGAGGAAAACGCTGCCTCTTACCACGGCCCCGGAGGCAGGGGAGGTAACCCCATCGGA TAGCGGGGGGCAAGAGGACTCTCCAGCCAAAGGGCTCTCCGTAAGGCTGGAGTTTGACTATTCTGAGGAC AAGAGTAGTTGGGACAACCAGCAGGAAAACCCCCCTCCTACCAAAAAGATAGGCAAAAAGCCAGTTGCCA AAATGCCCCTGAGGAGGCCAAAGATGAAAAAGACACCCGAGAAACTTGACAACACTCCTGCCTCACCTCC CAGATCCCCTGCTGAACCCAATGACATCCCCATTGCTAAAGGTACTTACACCTTTGATATTGACAAGTGG GATGACCCCAATTTTAACCCTTTTTCTTCCACCTCAAAAATGCAGGAGTCTCCCAAACTGCCCCAACAAT CATACAACTTTGACCCAGACACCTGTGATGAGTCCGTTGACCCCTTTAAGACATCCTCTAAGACCCCCAG CTCACCTTCTAAATCCCCAGCCTCCTTTGAGATCCCAGCCAGTGCTATGGAAGCCAATGGAGTGGACGGG GATGGGCTAAACAAGCCCGCCAAGAAGAAGAAGACGCCCCTAAAGACGATGGTTGAAGATGTGATGTCTG TGTGTTCTCTGTTTGACACATTTAGGGTGAAAAAGTCGCCAAAACGGTCTCCTCTCTCTGATCCACCTTC CCAGGACCCCACCCCAGCTGCTACACCAGAAACACCACCAGTGATCTCTGCGGTGGTCCACGCCACAGAT GAGGAAAAGCTGGCGGTCACCAACCAGAAGTGGACGTGCATGACAGTGGACCTAGAGGCTGACAAACAGG ACTACCCGCAGCCCTCGGACCTGTCCACCTTTGTAAACGAGACCAAATTCAGTTCACCCACTGAGGAGTT GGATTACAGAAACTCCTATGAAATTGAATATATGGAGAAAATTGGCTCCTCCTTACCTCAGGACGACGAT GCCCCGAAGAAGCAGGCCTTGTACCTTATGTTTGACACTTCTCAGGAGAGCCCTGTCAAGTCATCTCCCG TCCGCATGTCAGAGTCCCCGACGCCGTGTTCAGGGTCAAGTTTTGAAGAGACTGAAGCCCTTGTGAACAC TGCTGCGAAAAACCAGCATCCTGTCCCACGAGGACTGGCCCCTAACCAAGAGTCACACTTGCAGGTGCCA GAGAAATCCTCCCAGAAGGAGCTGGAGGCCATGGGCTTGGGCACCCCTTCAGAAGCGATTGAAATTACAG CTCCCGAGGGCTCCTTTGCCTCTGCTGACGCCCTCCTCAGCAGGCTAGCTCACCCCGTCTCTCTCTGTGG TGCACTTGACTATCTGGAGCCCGACTTAGCAGAAAAGAACCCCCCACTATTCGCTCAGAAACTCCAGAGA GAGGCTGCTCACCCAACAGACGTCTCCATCTCCAAAACAGCCTTGTACTCCCGCATCGGGACCGCTGAGG TGGAGAAACCTGCAGGCCTTCTGTTCCAGCAGCCCGACCTGGACTCTGCCCTCCAGATCGCCAGAGCAGA GATCATAACCAAGGAGAGAGAGGTCTCAGAATGGAAAGATAAATATGAAGAAAGCAGGCGGGAAGTGATG GAAATGAGGAAAATAGTGGCCGAGTATGAGAAGACCATCGCTCAGATGATAGAGGACGAACAGAGAGAGA AGTCAGTCTCCCACCAGACGGTGCAGCAGCTGGTTCTGGAGAAGGAGCAAGCCCTGGCCGACCTGAACTC CGTGGAGAAGTCTCTGGCCGACCTCTTCAGAAGATATGAGAAGATGAAGGAGGTCCTAGAAGGCTTCCGC AAGAATGAAGAGGTGTTGAAGAGATGTGCGCAGGAGTACCTGTCCCGGGTGAAGAAGGAGGAGCAGAGGT ACCAGGCCCTGAAGGTGCACGCGGAGGAGAAACTGGACAGGGCCAATGCTGAGATTGCTCAGGTTCGAGG CAAGGCCCAGCAGGAGCAAGCCGCCCACCAGGCCAGCCTGCGGAAGGAGCAGCTGCGAGTGGACGCCCTG GAAAGGACGCTGGAGCAGAAGAATAAAGAAATAGAAGAACTCACCAAGATTTGTGACGAACTGATTGCCA AAATGGGGAAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239878 representing NM_001291878 Red=Cloning site Green=Tags(s) MGGSQSLQPAPASDLNLEASEAMSSDSEEAFETPESTTPVKAPPAPPPPPPEVIPEPEVSTQPPPEEPGC GSETVPVPDGPRSDSVEGSPFRPPSHSFSAVFDEDKPIASSGTYNLDFDNIELVDTFQTLEPRASDAKNQ EGKVNTRRKSTDSVPISKSTLSRSLSLQASDFDGASSSGNPEAVALAPDAYSTGSSSASSTLKRTKKPRP PSLKKKQTTKKPTETPPVKETQQEPDEESLVPSGENLASETKTESAKTEGPSPALLEETPLEPAVGPKAA CPLDSESAEGVVPPASGGGRVQNSPPVGRKTLPLTTAPEAGEVTPSDSGGQEDSPAKGLSVRLEFDYSED KSSWDNQQENPPPTKKIGKKPVAKMPLRRPKMKKTPEKLDNTPASPPRSPAEPNDIPIAKGTYTFDIDKW DDPNFNPFSSTSKMQESPKLPQQSYNFDPDTCDESVDPFKTSSKTPSSPSKSPASFEIPASAMEANGVDG DGLNKPAKKKKTPLKTMVEDVMSVCSLFDTFRVKKSPKRSPLSDPPSQDPTPAATPETPPVISAVVHATD EEKLAVTNQKWTCMTVDLEADKQDYPQPSDLSTFVNETKFSSPTEELDYRNSYEIEYMEKIGSSLPQDDD APKKQALYLMFDTSQESPVKSSPVRMSESPTPCSGSSFEETEALVNTAAKNQHPVPRGLAPNQESHLQVP EKSSQKELEAMGLGTPSEAIEITAPEGSFASADALLSRLAHPVSLCGALDYLEPDLAEKNPPLFAQKLQR EAAHPTDVSISKTALYSRIGTAEVEKPAGLLFQQPDLDSALQIARAEIITKEREVSEWKDKYEESRREVM EMRKIVAEYEKTIAQMIEDEQREKSVSHQTVQQLVLEKEQALADLNSVEKSLADLFRRYEKMKEVLEGFR KNEEVLKRCAQEYLSRVKKEEQRYQALKVHAEEKLDRANAEIAQVRGKAQQEQAAHQASLRKEQLRVDAL ERTLEQKNKEIEELTKICDELIAKMGKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291878
ORF Size	3024 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291878.2
RefSeq Size	4036 bp
RefSeq ORF	3027 bp
Locus ID	10579
UniProt ID	O95359
MW	110.5 kDa
Gene Summary	Transforming acidic coiled-coil proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. This gene encodes a protein that concentrates at centrosomes throughout the cell cycle. This gene lies within a chromosomal region associated with tumorigenesis. Expression of this gene is induced by erythropoietin and is thought to affect the progression of breast tumors. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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