DIS (CCAR1) (NM_001282960) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240000 representing NM_001282960 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAATTTGGAGGACAGAAGAATCCGCCATGGGCTACTCAGTTTACAGCCACTGCAGTATCACAGC CAGCTGCACTGGGTGTTCAACAGCCATCACTCCTTGGAGCATCTCCTACCATTTATACACAGCAAACTGC ATTGGCAGCAGCAGGCCTTACCACACAAACTCCAGCAAACTATCAGTTAACACAAACTGCTGCATTGCAG CAACAAGCCGCAGCTGCAGCAGCTGCATTACAACAGTTACAGCAACCCCAGCAAACCCTCTTAACACAGC CAGCTGTTGCACTGCCTACAAGCCTTAGCCTGTCTACTCCTCAGCCAACAGCACAAATAACTGTATCATA TCCAACACCAAGGTCCAGTCAACAGCAAACCCAGCCTCAGAAGCAGCGTGTTTTCACAGGGGTGGTTACA AAACTACATGATACGTTTGGATTTGTGGATGAAGATGTATTCTTTCAGCTTAGTGCTGTCAAAGGGAAAA CCCCCCAAGTAGGTGACAGAGTATTGGTTGAAGCTACTTATAATCCTAATATGCCTTTTAAATGGAATGC ACAGAGAATTCAAACACTACCAAATCAGAATCAGTCGCAAACCCAGCCATTACTGAAGACTCCTCCTGCT GTACTTCAGCCAATTGCACCACAGACAACATTTGGTGTTCAGACTCAGCCCCAGCCCCAGTCACTGCTGC AGGCACAGATTTCAGCAGCTTCTATTACACCACTATTGCAGACTCAACCACAGCCCTTATTACAGCAGCC TCAGCAAAAAGCTGGTTTATTGCAGCCTCCTGTTCGTATAGTTTCACAGCCACAACCGGCACGACGATTA GATCCCCCATCCCGATTTTCAGGAAGAAATGACAGAGGGGATCAAGTGCCTAACAGAAAAGATGATCGAA GTCGTGAGAGAGAGAGAGAAAGACGTAGATCGAGAGAAAGATCACCTCAGAGGAAACGTTCCCGGGAAAG ATCTCCACGAAGAGAGCGAGAGCGATCACCTCGGAGAGTTCGACGTGTTGTTCCACGTTACACAGTTCAG TTTTCAAAGTTTTCTTTAGATTGTCCCAGTTGTGACATGATGGAACTAAGGCGCCGTTATCAAAATTTGT ATATACCTAGTGACTTTTTTGATGCTCAATTTACATGGGTGGATGCTTTCCCTTTGTCAAGACCATTTCA GCTGGGAAATTACTGCAATTTTTATGTAATGCACAGAGAAGTAGAGTCCTTAGAAAAAAATATGGCCATT CTTGATCCACCAGATGCTGACCACTTATACAGTGCAAAGGTAATGCTGATGGCTAGCCCTAGTATGGAAG ATTTATATCATAAGTCATGTGCTCTTGCTGAGGACCCACAAGAACTTCGAGATGGATTCCAACATCCTGC TAGACTTGTTAAGTTTTTAGTGGGCATGAAAGGCAAGGATGAAGCTATGGCCATTGGAGGCCACTGGTCT CCTTCGTTGGATGGACCAGACCCAGAAAAAGATCCCTCTGTGTTGATTAAGACTGCTATTCGTTGTTGTA AGGCTCTGACAGGCATTGATCTAAGTGTGTGCACACAATGGTACCGTTTTGCAGAGATTCGCTACCATCG CCCTGAGGAGACCCACAAGGGGCGTACAGTTCCAGCTCATGTGGAGACAGTGGTTTTATTTTTCCCGGAT GTTTGGCATTGCCTTCCCACCCGCTCAGAGTGGGAAACCCTCTCCCGAGGATACAAGCAGCAGCTGGTCG AGAAGCTTCAGGGTGAACGCAAGGAGGCTGATGGAGAACAGGATGAAGAAGAGAAGGATGATGGTGAAGC TAAAGAAATTTCTACACCTACCCATTGGTCTAAACTTGATCCAAAGACAATGAAGGTAAATGACCTCCGA AAAGAATTAGAAAGTCGAGCTCTTAGTTCCAAAGGATTAAAATCCCAGTTAATAGCCCGATTGACAAAAC AGCTTAAAGTAGAGGAACAAAAAGAAGAACAGAAGGAGTTAGAGAAATCTGAAAAAGAAGAGGATGAGGA TGATGATAGGAAATCTGAAGACGATAAAGAGGAAGAAGAAAGGAAACGTCAAGAGGAAATAGAACGCCAG CGTCGAGAAAGAAGATATATTTTGCCTGATGAACCGGCCATCATTGTACATCCAAATTGGGCTGCAAAAA GTGGCAAGTTTGATTGTAGCATCATGTCTTTGAGTGTCCTATTGGACTACAGATTAGAGGATAATAAAGA ACATTCATTTGAGGTTTCATTGTTTGCGGAACTTTTCAACGAAATGCTTCAAAGAGATTTTGGTGTCCGT ATATACAAATCATTACTGTCTCTTCCTGAGAAAGAGGACAAAAAAGAAAAGGATAAAAAAAGCAAAAAAG ATGAGAGAAAAGATAAAAAAGAAGAAAGAGATGATGAAACTGATGAACCAAAACCCAAACGGAGAAAATC AGGCGATGATAAAGATAAAAAAGAAGATAGAGATGAAAGGAAGAAAGAAGATAAAAGAAAAGATGATTCT AAAGATGATGATGAAACTGAAGAAGATAACAATCAAGATGAATATGACCCTATGGAAGCAGAAGAAGCTG AGGATGAAGAAGATGATAGGGATGAGGAAGAAATGACCAAACGAGATGACAAAAGAGATATCAACAGATA CTGCAAGGAGAGGCCCTCTAAAGATAAGGAAAAAGAAAAGACTCAAATGATCACAATTAACAGAGATCTG TTAATGGCTTTTGTTTATTTTGATCAAAGTCATTGTGGTTACCTTCTTGAAAAGGATTTGGAAGAAATAC TTTATACTCTTGGACTACATCTTTCTCGGGCTCAGGTAAAGAAGCTTCTTAATAAAGTAGTGCTCCGTGA ATCTTGCTTTTACCGGAAATTAACAGACACCTCAAAAGATGAAGAGAACCATGAAGAGTCTGAGTCATTG CAGGAAGATATGCTAGGAAACAGATTATTACTTCCAACACCAACAGTAAAGCAGGAATCAAAGGATGTGG AAGAAAATGTTGGCCTCATTGTGTACAATGGTGCAATGGTAGATGTAGGAAGCCTCTTGCAAAAATTGGA AAAGAGCGAAAAAGTAAGAGCTGAGGTAGAACAGAAGCTGCAGTTACTAGAAGAAAAAACAGATGAAGAT GAAAAAACCATATTAAATTTGGAGAATTCCAACAAAAGCCTCTCTGGTGAACTCAGAGAAGTTAAAAAGG ACCTTAGTCAGTTACAAGAAAACTTAAAGATTTCGGAAAACATGAATTTACAATTTGAAAACCAAATGAA TAAGACAATCAGAAACTTATCTACGGTAATGGATGAAATCCACACTGTTCTCAAGAAGGATAATGTAAAG AATGAAGACAAAGATCAAAAATCCAAGGAGAATGGTGCCAGTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240000 representing NM_001282960 Red=Cloning site Green=Tags(s) MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQ QQAAAAAAALQQLQQPQQTLLTQPAVALPTSLSLSTPQPTAQITVSYPTPRSSQQQTQPQKQRVFTGVVT KLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPPA VLQPIAPQTTFGVQTQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRL DPPSRFSGRNDRGDQVPNRKDDRSRERERERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQ FSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNMAI LDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQELRDGFQHPARLVKFLVGMKGKDEAMAIGGHWS PSLDGPDPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPD VWHCLPTRSEWETLSRGYKQQLVEKLQGERKEADGEQDEEEKDDGEAKEISTPTHWSKLDPKTMKVNDLR KELESRALSSKGLKSQLIARLTKQLKVEEQKEEQKELEKSEKEEDEDDDRKSEDDKEEEERKRQEEIERQ RRERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVR IYKSLLSLPEKEDKKEKDKKSKKDERKDKKEERDDETDEPKPKRRKSGDDKDKKEDRDERKKEDKRKDDS KDDDETEEDNNQDEYDPMEAEEAEDEEDDRDEEEMTKRDDKRDINRYCKERPSKDKEKEKTQMITINRDL LMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDEENHEESESL QEDMLGNRLLLPTPTVKQESKDVEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDED EKTILNLENSNKSLSGELREVKKDLSQLQENLKISENMNLQFENQMNKTIRNLSTVMDEIHTVLKKDNVK NEDKDQKSKENGASV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282960
ORF Size	3405 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282960.2
RefSeq Size	4724 bp
RefSeq ORF	3408 bp
Locus ID	55749
UniProt ID	Q8IX12
MW	131.5 kDa
Gene Summary	Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed:12816952). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).[UniProtKB/Swiss-Prot Function]

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