CEP162 (NM_001286206) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C6orf84; KIAA1009; QN1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240111 representing NM_001286206 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATAGAAGAGGAGTCTGCTGAAAAGATTCAATTTCTTAAGAGCAGTGGAACCTCTCTCTTAAGTA CTGATAGCTTAGAAACAAATGAACTAGTAGTTTCTGAGCTCAACCATAGTAGTCTCGGAGTGGGATTGGA CACATTAGAAGAACAAGAGGAGAAAGAACAATTTTTTGCCAGGCTTGAGAAAGGCTTGACATCTTCCATT GATTATTCGAGATTAAATAAGGAATTGGATTCTAATGACTCTACACATTTTAAAGCTTTACATAGTAATC AAGCCAACGCAGAACTAACTGATGACGAACATGAGAATGAATCGAAACATGAAGAACTGGCAGAAAATTA CAGTGATGATTTTGAAGATGAGTATGTTGGTGCACCGTTGACTACTAAAGATGAAGAGATGCCTTCCAAA GAGAATTCCAAATCAGAAAAAATAAGTGTGCCCAAACAGGAAGAAGAAAAAACTGGCATGCTTGCTAATG TTGTGCTGCTTGATTCATTAGACTCTGTTGCAGAGGTCAATCTTGATGAACAAGATAAAATAACACCTAA GCCAAGGTGCCTACCAGAAATGACTGAGAATGAAATGACAGGAACAGGTGTTTCTTATGGACAAAGCAGT AGTGACGTTGAAGCCCTACATCAAGCTTATTGTCATATAGCCCATTCATTGGGAGATGAAGACAAACAAA AAATTGAGAGTAACACAGTGGAAGATATCAAGAGCTCAGTGAAAGGTCATCCTCAAGAAAATGAAGAGAA TTCAAAAAACATCTCTACTATGGAATCTGATCTGCCCACAGTAGAGGAGCTGATGAAACCTATCAGAATA GATTCCTTTGGGATCAGTGGTTTTGATTTACAACCTGTCAGCTCTGAGAAAGTGGCCGAAAGAAAAGAAA CTGAATTTTTTAGCTCTTTACCCCTGAAGATGAACCCAAATATTTTGTCTCAAGACTCACAACATGTGAA CCTTTTTTTTGACAAAAATGATGAGAATGTGATTTTACAAAAGACCACAAATGAGAGTATGGAAAACAGC TGTCCACAAGTAACTGAAGTAACTGCCACAGAAGAACATGTTGATAAAATGTACCTTAATATTTTGAGGA AAAAAATAACTGTTAATTCTTCATCATTATCTCAGGATGACAAAATTAATAAAACTTACAGATCTCAACT TAGTTCTGAAGAAGAAGGGGCTGTAATGGGTAAACAGGTACCATACAAGAAGGCCAGAAGTGCACCTCCT TTACTTAAAAGGAAACCCCAGAGTGGATTATATGCGTCAGTTAGGAGCTCAGGCTATGGCAAACCCAGTT CACCACTCAAGATGTTTTCTACTCTTGAAAAGAAAACTTCAGAGGACATTATAAAAAGCAAAAACTTGAG ATCGATTTCTACCTCCAATCAACCTAGGAAAAAAGAAATCTTATCTGGAACAAAACTCATCAAGCCTGCA GCTTTGGATAAACCAGCTCACAAAACTGAAAGTTGCCTGTCTACTCGTAAGAAGTCTGAAAATCCCACAG AAACTGATTCCTGTATTCAGTTTCAGACTGATTCCTTAGGATACTGTGGTGAGAACAAGGAGAAGAAATT ACTTATGTTTAAAAGAGTTCAGGAAGCAGAGGATAAATGGAGGGGTGCGCAAGCCCTAATTGAGCAAATT AAAGCCACATTCTCAGAAAAGGAGAAAGAACTAGAAAATAAGTTGGAAGAACTAAAGAAACAACAGGAAA AAGAACTCTTCAAATTGAATCAAGATAATTATATTCTTCAAGCCAAGTTAAGCAGCTTTGAAGAAACAAA CAAAAAACAAAGGTGGTTACATTTTGGAGAAGCAGCTGATCCTGTCACTGGAGAAAAGTTGAAGCAAATC CAAAAAGAAATACAAGAACAAGAGACACTTCTTCAAGGATATCAACAGGAAAATGAACGATTATATAATC AAGTAAAAGATCTCCAAGAACAAAACAAGAAAAATGAGGAGCGAATGTTTAAGGAAAACCAAAGTTTATT CAGTGAGGTAGCTTCCTTAAAAGAACAGATGCACAAAAGTCGTTTTCTGTCTCAAGTAGTTGAAGATTCA GAGCCCACAAGAAATCAGAATTTTACAGATCTGTTAGCAGAACTACGGATGGCACAGAAAGAAAAAGACA GTTTATTGGAAGACATCAAAAGACTGAAACAAGACAAACAAGCTCTTGAAGTAGACTTCGAAAAAATGAA GAAAGAGAGGGACCAAGCCAAAGATCAGATTGCTTATGTCACAGGTGAGAAATTATATGAAATAAAAATT TTAGAAGAAACACATAAACAAGAAATCAGTCGTCTGCAAAAAAGATTACAGTGGTATGCTGAAAATCAGG AACTTCTGGATAAAGATGCACTTCGGCTTAGAGAAGCAAATGAGGAAATTGAGAAGCTCAAACTTGAGAT TGAGAAACTGAAAGCTGAATCTGGGAATCCATCTATTCGGCAGAAGATACGCTTAAAAGATAAAGCAGCA GATGCCAAAAAAATTCAGGATCTGGAGCGACAAGTTAAGGAAATGGAAGGGATTCTGAAGAGAAGATATC CCAATTCTTTACCTGCTTTAATATTGGCTGCATCAGCAGCTGGTGATACAGTGGATAAAAATACAGTGGA ATTTATGGAGAAAAGGATAAAAAAGCTAGAAGCTGATCTGGAGGGCAAAGATGAAGATGCAAAGAAAAGC CTTCGTACCATGGAACAACAGTTTCAGAAAATGAAGATTCAGTATGAACAAAGACTAGAGCAGCAGGAGC AGCTACTTGCCTGCAAATTGAATCAACATGACTCTCCCAGAATTAAAGCCCTAGAGAAGGAACTTGATGA CATCAAGGAAGCCCATCAGATCACTGTAAGAAACCTTGAAGCCGAAATAGACGTTCTTAAACATCAGAAT GCTGAATTAGACGTCAAGAAAAATGATAAAGATGATGAAGATTTTCAGTCTATAGAATTCCAGGTGGAAC AGGCTCATGCTAAAGCTAAATTAGTAAGACTCAATGAAGAACTGGCTGCAAAAAAGAGAGAAATACAAGA CCTCTCAAAAACTGTGGAGAGGCTTCAGAAAGACAGAAGAATGATGCTATCAAATCAGAACTCAAAGGGC AGAGAGGAAATGTCTGCCAAAAGGGCAAAGAAAGATGTTTTGCACTCAAGTAAAGGAAATGCTAACTCCT TCCCTGGAACCCTGGACAGCAAGCTGTACCAACCACATACTTTCACTGATTCCCATGTTTCAGAAGTTTT ACAAGAAAACTACAGATTAAAAAATGAGCTAGAAGGATTAATTTCAGAGAAGAATGAACTGAAGATGAAA TCTGAAGCAGTGATGAATCAATTTGAAAACTCCATGAGAAGGGTCAAGGAAGATACTGCAGCACACATTG CATCCCTCAAAGCATCTCATCAGAGAGAAATAGAGAAACTCCTTTGCCAAAATGCAGTAGAAAATTCTTC TTCCAAAGTAGCTGAACTAAATCGTAAAATAGCAACTCAAGAGGTACTTATAAGACATTTCCAAAGTCAA GTTAATGAGCTGCAGAGTAAACAAGAGTCTCTCGTAGTTTCTGAAGTTCGAGAAGAAATTCTACAGAAAG AGATTACAAAACTCTTAGAAGAATTGAGAGAAGCCAAAGAAAACCATACACCAGAGATGAAACATTTCGT GGGCTTAGAAAAGAAAATTAAGCAGATGGAAATGAGACATGCACAAAGAGAACAGGAACTTCAACAGATA ATACAGCAAACACACCAAGTAGTAGAAACTGAGCAAAACAAAGAAGTTGAAAAATGGAAAAGACTGGCAC AGTTAAAGAATCGTGAGCTGGAGAAGTTCCGCACAGAACTAGACTCAATATTAGATGTTCTCCGAGAGCT GCACCGGCAAGGAGTGGTTGTGCCAGTTGCTTTTGCAGATGAAATGAATGCACCAGAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240111 representing NM_001286206 Red=Cloning site Green=Tags(s) MEIEEESAEKIQFLKSSGTSLLSTDSLETNELVVSELNHSSLGVGLDTLEEQEEKEQFFARLEKGLTSSI DYSRLNKELDSNDSTHFKALHSNQANAELTDDEHENESKHEELAENYSDDFEDEYVGAPLTTKDEEMPSK ENSKSEKISVPKQEEEKTGMLANVVLLDSLDSVAEVNLDEQDKITPKPRCLPEMTENEMTGTGVSYGQSS SDVEALHQAYCHIAHSLGDEDKQKIESNTVEDIKSSVKGHPQENEENSKNISTMESDLPTVEELMKPIRI DSFGISGFDLQPVSSEKVAERKETEFFSSLPLKMNPNILSQDSQHVNLFFDKNDENVILQKTTNESMENS CPQVTEVTATEEHVDKMYLNILRKKITVNSSSLSQDDKINKTYRSQLSSEEEGAVMGKQVPYKKARSAPP LLKRKPQSGLYASVRSSGYGKPSSPLKMFSTLEKKTSEDIIKSKNLRSISTSNQPRKKEILSGTKLIKPA ALDKPAHKTESCLSTRKKSENPTETDSCIQFQTDSLGYCGENKEKKLLMFKRVQEAEDKWRGAQALIEQI KATFSEKEKELENKLEELKKQQEKELFKLNQDNYILQAKLSSFEETNKKQRWLHFGEAADPVTGEKLKQI QKEIQEQETLLQGYQQENERLYNQVKDLQEQNKKNEERMFKENQSLFSEVASLKEQMHKSRFLSQVVEDS EPTRNQNFTDLLAELRMAQKEKDSLLEDIKRLKQDKQALEVDFEKMKKERDQAKDQIAYVTGEKLYEIKI LEETHKQEISRLQKRLQWYAENQELLDKDALRLREANEEIEKLKLEIEKLKAESGNPSIRQKIRLKDKAA DAKKIQDLERQVKEMEGILKRRYPNSLPALILAASAAGDTVDKNTVEFMEKRIKKLEADLEGKDEDAKKS LRTMEQQFQKMKIQYEQRLEQQEQLLACKLNQHDSPRIKALEKELDDIKEAHQITVRNLEAEIDVLKHQN AELDVKKNDKDDEDFQSIEFQVEQAHAKAKLVRLNEELAAKKREIQDLSKTVERLQKDRRMMLSNQNSKG REEMSAKRAKKDVLHSSKGNANSFPGTLDSKLYQPHTFTDSHVSEVLQENYRLKNELEGLISEKNELKMK SEAVMNQFENSMRRVKEDTAAHIASLKASHQREIEKLLCQNAVENSSSKVAELNRKIATQEVLIRHFQSQ VNELQSKQESLVVSEVREEILQKEITKLLEELREAKENHTPEMKHFVGLEKKIKQMEMRHAQREQELQQI IQQTHQVVETEQNKEVEKWKRLAQLKNRELEKFRTELDSILDVLRELHRQGVVVPVAFADEMNAPEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286206
ORF Size	3981 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286206.2
RefSeq Size	5119 bp
RefSeq ORF	3984 bp
Locus ID	22832
UniProt ID	Q5TB80
MW	153.5 kDa
Gene Summary	Required to promote assembly of the transition zone in primary cilia. Acts by specifically recognizing and binding the axonemal microtubule. Localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base. Required to mediate CEP290 association with microtubules.[UniProtKB/Swiss-Prot Function]

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