SLIT2 (NM_001289136) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SLIL3; Slit-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240170 representing NM_001289136 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGGGTTAGTGCTGGCGATCCTGAACAAGGTGG CACCGCAGGCGTGCCCGGCGCAGTGCTCTTGCTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTGCG CAGCGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGAATT ACGAAGACAGATTTTGCTGGTCTTAGACATCTAAGAGTTCTTCAGCTTATGGAGAATAAGATTAGCACCA TTGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCACCTTCAGCT GTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCTCAGTGAAAACCAAATTCAG GCAATCCCAAGGAAAGCTTTCCGTGGGGCAGTTGACATAAAAAATTTGCAACTGGATTACAACCAGATCA GCTGTATTGAAGATGGGGCATTCAGGGCTCTCCGGGACCTGGAAGTGCTCACTCTCAACAATAACAACAT TACTAGACTTTCTGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACTTTTCGACTGCATTCAAACAAC CTGTATTGTGACTGCCACCTGGCCTGGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCTGTACA CTCAGTGTATGGGCCCCTCCCACCTGAGAGGCCATAATGTAGCCGAGGTTCAAAAACGAGAATTTGTCTG CAGTGGTCACCAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTGCCCTGCCGCCTGTACCTGTAGC AACAATATCGTAGACTGTCGTGGGAAAGGTCTCACTGAGATCCCCACAAATCTTCCAGAGACCATCACAG AAATACGTTTGGAACAGAACACAATCAAAGTCATCCCTCCTGGAGCTTTCTCACCATATAAAAAGCTTAG ACGAATTGACCTGAGCAATAATCAGATCTCTGAACTTGCACCAGATGCTTTCCAAGGACTACGCTCTCTG AATTCACTTGTCCTCTATGGAAATAAAATCACAGAACTCCCCAAAAGTTTATTTGAAGGACTGTTTTCCT TACAGCTCCTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCAGGATCTCCACAA CTTGAACCTTCTCTCCCTATATGACAACAAGCTTCAGACCATCGCCAAGGGGACCTTTTCACCTCTTCGG GCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTATTTGTGACTGCCATCTCAAGTGGCTAGCGGATT ATCTCCATACCAACCCGATTGAGACCAGTGGTGCCCGTTGCACCAGCCCCCGCCGCCTGGCAAACAAAAG AATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGATTATCGATCAAAATTAAGTGGA GACTGCTTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGAAGGAACCACAGTAGATTGCTCTAATC AAAAGCTCAACAAAATCCCGGAGCACATTCCCCAGTACACTGCAGAGTTGCGTCTCAATAATAATGAATT TACCGTGTTGGAAGCCACAGGAATCTTTAAGAAACTTCCTCAATTACGTAAAATAAACTTTAGCAACAAT AAGATCACAGATATTGAGGAGGGAGCATTTGAAGGAGCATCTGGTGTAAATGAAATACTTCTTACGAGTA ATCGTTTGGAAAATGTGCAGCATAAGATGTTCAAGGGATTGGAAAGCCTCAAAACTTTGATGTTGAGAAG CAATCGAATAACCTGTGTGGGGAATGACAGTTTCATAGGACTCAGTTCTGTGCGTTTGCTTTCTTTGTAT GATAATCAAATTACTACAGTTGCACCAGGGGCATTTGATACTCTCCATTCTTTATCTACTCTAAACCTCT TGGCCAATCCTTTTAACTGTAACTGCTACCTGGCTTGGTTGGGAGAGTGGCTGAGAAAGAAGAGAATTGT CACGGGAAATCCTAGATGTCAAAAACCATACTTCCTGAAAGAAATACCCATCCAGGATGTGGCCATTCAG GACTTCACTTGTGATGACGGAAATGATGACAATAGTTGCTCCCCACTTTCTCGCTGTCCTACTGAATGTA CTTGCTTGGATACAGTCGTCCGATGTAGCAACAAGGGTTTGAAGGTCTTGCCGAAAGGTATTCCAAGAGA TGTCACAGAGTTGTATCTGGATGGAAACCAATTTACACTGGTTCCCAAGGAACTCTCCAACTACAAACAT TTAACACTTATAGACTTAAGTAACAACAGAATAAGCACGCTTTCTAATCAGAGCTTCAGCAACATGACCC AGCTCCTCACCTTAATTCTTAGTTACAACCGTCTGAGATGTATTCCTCCTCGCACCTTTGATGGATTAAA GTCTCTTCGATTACTTTCTCTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCTTTCAATGATCTT TCTGCATTATCACATCTAGCAATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCG ACTGGGTGAAGTCGGAATATAAGGAGCCTGGAATTGCTCGTTGTGCTGGTCCTGGAGAAATGGCAGATAA ACTTTTACTCACAACTCCCTCCAAAAAATTTACCTGTCAAGGTCCTGTGGATGTCAATATTCTAGCTAAG TGTAACCCCTGCCTATCAAATCCGTGTAAAAATGATGGCACATGTAATAGTGATCCAGTTGACTTTTACC GATGCACCTGTCCATATGGTTTCAAGGGGCAGGACTGTGATGTCCCAATTCATGCCTGCATCAGTAACCC ATGTAAACATGGAGGAACTTGCCACTTAAAGGAAGGAGAAGAAGATGGATTCTGGTGTATTTGTGCTGAT GGATTTGAAGGAGAAAATTGTGAAGTCAACGTTGATGATTGTGAAGATAATGACTGTGAAAATAATTCTA CATGTGTCGATGGCATTAATAACTACACATGCCTTTGCCCACCTGAGTATACAGGTGAGTTGTGTGAGGA GAAGCTGGACTTCTGTGCCCAGGACCTGAACCCCTGCCAGCACGATTCAAAGTGCATCCTAACTCCAAAG GGATTCAAATGTGACTGCACACCAGGGTACGTAGGTGAACACTGCGACATCGATTTTGACGACTGCCAAG ACAACAAGTGTAAAAACGGAGCCCACTGCACAGATGCAGTGAACGGCTATACGTGCATATGCCCCGAAGG TTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGTACCAGCCCCTGTGATAATTTT GATTGTCAGAATGGAGCTCAGTGTATCGTCAGAATAAATGAGCCAATATGTCAGTGTTTGCCTGGCTATC AGGGAGAAAAGTGTGAAAAATTGGTTAGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGATTCCTTC AGCCAAGGTTCGGCCTCAGACGAACATAACACTTCAGATTGCCACAGATGAAGACAGCGGAATCCTCCTG TATAAGGGTGACAAAGACCATATCGCGGTAGAACTCTATCGGGGGCGTGTTCGTGCCAGCTATGACACCG GCTCTCATCCAGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAACT ACTTGCCTTGGATCAGAGTCTCTCTTTGTCCGTGGATGGTGGGAACCCCAAAATCATCACTAACTTGTCA AAGCAGTCCACTCTGAATTTTGACTCTCCACTCTATGTAGGAGGCATGCCAGGGAAGAGTAACGTGGCAT CTCTGCGCCAGGCCCCTGGGCAGAACGGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACATCAACAG TGAGCTGCAGGACTTCCAGAAGGTGCCGATGCAAACAGGCATTTTGCCTGGCTGTGAGCCATGCCACAAG AAGGTGTGTGCCCATGGCACATGCCAGCCCAGCAGCCAGGCAGGCTTCACCTGCGAGTGCCAGGAAGGAT GGATGGGGCCCCTCTGTGACCAACGGACCAATGACCCTTGCCTTGGAAATAAATGCGTACATGGCACCTG CTTGCCCATCAATGCGTTCTCCTACAGCTGTAAGTGCTTGGAGGGCCATGGAGGTGTCCTCTGTGATGAA GAGGAGGATCTGTTTAACCCATGCCAGGCGATCAAGTGCAAGCATGGGAAGTGCAGGCTTTCAGGTCTGG GGCAGCCCTACTGTGAATGCAGCAGTGGATACACGGGGGACAGCTGTGATCGAGAAATCTCTTGTCGAGG GGAAAGGATAAGAGATTATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAACAACCAAGAAGGTGTCC CGATTAGAGTGCAGAGGTGGGTGTGCAGGAGGGCAGTGCTGTGGACCGCTGAGGAGCAAGCGGCGGAAAT ACTCTTTCGAATGCACTGACGGCTCCTCCTTTGTGGACGAGGTTGAGAAAGTGGTGAAGTGCGGCTGTAC GAGGTGTGTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240170 representing NM_001289136 Red=Cloning site Green=Tags(s) MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRI TKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQ AIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNN LYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCS NNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSL NSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLR AIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNN KITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLY DNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQ DFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKH LTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDL SALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAK CNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICAD GFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPK GFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNF DCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILL YKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLS KQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHK KVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDE EEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVS RLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289136
ORF Size	4563 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289136.3
RefSeq Size	6374 bp
RefSeq ORF	4566 bp
Locus ID	9353
UniProt ID	O94813
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Axon guidance
MW	169.3 kDa
Gene Summary	This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

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