TMEM147 (NM_032635) Human Tagged ORF Clone
CAT#: RG200560
- TrueORF®
TMEM147 (tGFP-tagged) - Human transmembrane protein 147 (TMEM147)
ORF Plasmid: DDK
"NM_032635" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
CNY 6,281.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NIFIE14 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200560 representing NM_032635
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGTTTCACTTCGGGAACTGCTTCGCTCTTGCCTACTTCCCCTACTTCATCACCTACAAGTGCA GCGGCCTGTCCGAGTACAACGCCTTCTGGAAATGCGTCCAGGCTGGAGTCACCTACCTCTTTGTCCAACT CTGCAAGATGCTGTTCTTGGCCACTTTCTTTCCCACCTGGGAAGGCGGCATCTATGACTTCATTGGGGAG TTCATGAAGGCCAGCGTGGATGTGGCAGACCTGATAGGTCTAAACCTTGTCATGTCCCGGAATGCCGGCA AGGGAGAGTACAAGATCATGGTTGCTGCCCTGGGCTGGGCCACTGCTGAGCTTATTATGTCCCGCTGCAT TCCCCTATGGGTCGGAGCCCGGGGCATTGAGTTTGACTGGAAGTACATCCAGATGAGCATAGACTCCAAC ATCAGTCTGGTCCATTACATCGTCGCGTCTGCTCAGGTCTGGATGATAACACGCTATGATCTGTACCACA CCTTCCGGCCAGCTGTCCTCCTGCTGATGTTCCTCAGTGTCTACAAGGCCTTTGTTATGGAGACCTTCGT CCACCTCTGCTCGCTGGGCAGTTGGGCAGCTCTACTGGCCCGAGCAGTGGTAACGGGGCTGCTGGCCCTC AGCACTTTGGCCCTGTATGTCGCCGTTGTCAATGTGCACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200560 representing NM_032635
Red=Cloning site Green=Tags(s) MTLFHFGNCFALAYFPYFITYKCSGLSEYNAFWKCVQAGVTYLFVQLCKMLFLATFFPTWEGGIYDFIGE FMKASVDVADLIGLNLVMSRNAGKGEYKIMVAALGWATAELIMSRCIPLWVGARGIEFDWKYIQMSIDSN ISLVHYIVASAQVWMITRYDLYHTFRPAVLLLMFLSVYKAFVMETFVHLCSLGSWAALLARAVVTGLLAL STLALYVAVVNVHS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032635 |
ORF Size | 672 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032635.4 |
RefSeq Size | 869 bp |
RefSeq ORF | 675 bp |
Locus ID | 10430 |
UniProt ID | Q9BVK8 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | Acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (PubMed:21056967). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (PubMed:21056967).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200560 | TMEM147 (Myc-DDK-tagged)-Human transmembrane protein 147 (TMEM147) |
CNY 2,400.00 |
|
RC200560L1 | Lenti ORF clone of Human transmembrane protein 147 (TMEM147), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200560L2 | Lenti ORF clone of Human transmembrane protein 147 (TMEM147), mGFP tagged |
CNY 5,890.00 |
|
RC200560L3 | Lenti ORF clone of Human transmembrane protein 147 (TMEM147), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200560L4 | Lenti ORF clone of Human transmembrane protein 147 (TMEM147), mGFP tagged |
CNY 5,890.00 |
|
SC104225 | TMEM147 (untagged)-Human transmembrane protein 147 (TMEM147) |
CNY 2,400.00 |
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