TMEM147 (NM_032635) Human Untagged Clone

CAT#: SC104225

TMEM147 (untagged)-Human transmembrane protein 147 (TMEM147)



  "NM_032635" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal antibody to Transmembrane protein 147 (transmembrane protein 147)
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms NIFIE14
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_032635, RT-PCR generated
GGCATCATGACCCTGTTTCACTTCGGGAACTGCTTCGCTCTTGCCTACTTCCCCTACTTC
ATCACCTACAAGTGCAGCGGCCTGTCCGAGTACAACGCCTTCTGGAAATGCGTCCAGGCT
GGAGTCACCTACCTCTTTGTCCAACTCTGCAAGATGCTGTTCTTGGCCACTTTCTTTCCC
ACCTGGGAAGGCGGCATCTATGACTTCATTGGGGAGTTCATGAAGGCCAGCGTGGATGTG
GCAGACCTGATAGGTCTAAACCTTGTCATGTCCCGGAATGCCGGCAAGGGAGAGTACAAG
ATCATGGTTGCTGCCCTGGGCTGGGCCACTGCTGAGCTTATTATGTCCCGCTGCATTCCC
CTATGGGTCGGAGCCCGGGGCATTGAGTTTGACTGGAAGTACATCCAGATGAGCATAGAC
TCCAACATCAGTCTGGTCCATTACATCGTCGCGTCTGCTCAGGTCTGGATGATAACACGC
TATGATCTGTACCACACCTTCCGGCCAGCTGTCCTCCTGCTGATGTTCCTCAGTGTCTAC
AAGGCCTTTGTTATGGAGACCTTCGTCCACCTCTGCTCGCTGGGCAGTTGGGCAGCTCTA
CTGGCCCGAGCAGTGGTAACGGGGCTGCTGGCCCTCAGCACTTTGGCCCTGTATGTCGCC
GTTGTCAATGTGCACTCCTAGG
>OriGene 5' read for NM_032635 unedited
GGGGTAGCTCAGTATTGTAAACGACTCATATAGGCGGCCGCGAATCACACTGGTACCGAG
CTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTGGCATCATGACCCTG
TTTCACTTCGGGAACTGCTTCGCTCTTGCCTACTTCCCCTACTTCATCACCTACAAGTGC
AGCGGCCTGTCCGAGTACAACGCCTTCTGGAAATGCGTCCAGGCTGGAGTCACCTACCTC
TTTGTCCAACTCTGCAAGATGCTGTTCTTGGCCACTTTCTTTCCCACCTGGGAAGGCGGC
ATCTATGACTTCATTGGGGAGTTCATGAAGGCCAGCGTGGATGTGGCAGACCTGATAGGT
CTAAACCTTGTCATGTCCCGGAATGCCGGCAAGGGAGAGTACAAGATCATGGTTGCTGCC
CTGGGCTGGGCCACTGCTGAGCTTATTATGTCCCGCTGCATTCCCCTATGGGTCGGAGCC
CGGGGCATTGAGTTTGACTGGAAGTACATCCAGATGAGCATAGACTCCAACATCAGTCTG
GTCCATTACATCGTCGCGTCTGCTCAGGTCTGGATGATAACACGCTATGATCTGTACCAC
ACCTTCCGGCCAGCTGTCCTCCTGCTGATGTTCCTCAGTGTCTACAAGGCCTTTGTTATG
GAGACCTTCGTCCACCTCTGCTCGCTGGGCAGTTGGGCAGCTCTACTGGCCCGAGCAGTG
GTAACGGGGCTGCTGGCCCTCAGCACTTTGGCCCTGTATGTCGCCGTTGTCAATGTGCAC
TCCTAGGAAGCCGAATTCTGCAGATATCCATCACACTGGCGGGCCGCGTCATAGCTGTTT
CCTGACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCATGCCTCTCCTGGCCCTGGGA
AGTGCCACT
Restriction Sites Please inquire     
ACCN NM_032635
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032635.2, NP_116024.1
RefSeq Size 869 bp
RefSeq ORF 675 bp
Locus ID 10430
UniProt ID Q9BVK8
Protein Families Druggable Genome, GPCR, Transmembrane
Gene Summary Acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (PubMed:21056967). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (PubMed:21056967).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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