SART1 (NM_005146) Human Tagged ORF Clone
CAT#: RG200603
- TrueORF®
SART1 (tGFP-tagged) - Human squamous cell carcinoma antigen recognized by T cells (SART1)
ORF Plasmid: DDK
"NM_005146" in other vectors (6)
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CNY 7,510.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Ara1; HAF; HOMS1; SART1259; SNRNP110; Snu66 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200603 representing NM_005146
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGTCCAAGAAGCATCGCGGAGAGAAGGAGGCGGCCGGGACGACGGCGGCGGCCGGCACCGGGG GTGCCACCGAGCAGCCGCCGCGGCACCGGGAACACAAAAAACACAAGCACCGGAGTGGCGGCAGTGGCGG TAGCGGTGGCGAACGACGGAAGCGGAGCCGGGAACGTGGGGGCGAGCGCGGGAGCGGGCGGCGCGGGGCC GAAGCTGAGGCCCGGAGCAGCACGCACGGGCGGGAGCGCAGCCAGGCAGAGCCCTCCGAGCGGCGCGTGA AGCGGGAGAAGCGCGATGACGGCTACGAGGCCGCTGCCAGCTCCAAAACTAGCTCAGGCGATGCCTCCTC ACTCAGCATCGAGGAGACTAACAAACTCCGGGCAAAGTTGGGGCTGAAACCCTTGGAGGTTAATGCCATC AAGAAGGAGGCGGGCACCAAGGAGGAGCCCGTGACAGCTGATGTCATCAACCCTATGGCCTTGCGACAGC GAGAGGAGCTGCGGGAGAAGCTGGCAGCTGCCAAGGAGAAGCGCCTGCTGAACCAAAAGCTGGGGAAGAT AAAGACCCTAGGAGAGGATGACCCCTGGCTGGACGACACTGCAGCCTGGATCGAGAGGAGCCGGCAGCTG CAGAAGGAGAAGGACCTGGCAGAGAAGAGGGCCAAGTTACTGGAGGAGATGGACCAAGAGTTTGGTGTCA GCACTCTGGTGGAGGAGGAGTTCGGGCAGAGGCGGCAGGACCTGTACAGTGCCCGGGACCTGCAGGGCCT CACCGTGGAGCATGCCATTGATTCCTTCCGAGAAGGGGAGACAATGATTCTTACCCTCAAGGACAAAGGC GTGCTGCAGGAGGAGGAGGACGTGCTGGTGAACGTGAACCTGGTGGATAAGGAGCGGGCAGAGAAAAATG TGGAGCTGCGGAAGAAGAAGCCTGACTACCTGCCCTATGCCGAGGACGAGAGCGTGGACGACCTGGCGCA GCAAAAACCTCGCTCTATCCTGTCCAAGTATGACGAAGAGCTTGAAGGGGAGCGGCCACATTCCTTCCGC TTGGAGCAGGGCGGCACGGCTGATGGCCTGCGGGAGCGGGAGCTGGAGGAGATCCGGGCCAAGCTGCGGC TGCAGGCTCAGTCCCTGAGCACAGTGGGGCCCCGGCTGGCCTCCGAATACCTCACGCCTGAGGAGATGGT GACCTTTAAAAAGACCAAGCGGAGGGTGAAGAAAATCCGCAAGAAGGAGAAGGAGGTAGTAGTGCGGGCA GATGACTTGCTGCCTCTCGGGGACCAGACTCAGGATGGGGACTTTGGTTCCAGACTGCGGGGACGGGGTC GCCGCCGAGTGTCCGAAGTGGAGGAGGAGAAGGAGCCTGTGCCTCAGCCCCTGCCGTCGGACGACACCCG AGTGGAGAACATGGACATCAGTGATGAGGAGGAAGGTGGAGCTCCACCGCCGGGGTCCCCGCAGGTGCTG GAGGAGGACGAGGCGGAGCTGGAGCTGCAGAAGCAGCTGGAGAAGGGACGCCGGCTGCGACAGTTACAGC AGCTACAGCAGCTGCGAGACAGTGGCGAGAAGGTGGTGGAGATTGTGAAGAAGCTGGAGTCTCGCCAGCG GGGCTGGGAGGAGGATGAGGATCCCGAGCGGAAGGGGGCCATCGTGTTCAACGCCACGTCCGAGTTCTGC CGCACCTTGGGGGAGATCCCCACCTACGGGCTGGCTGGCAATCGCGAGGAGCAGGAGGAGCTCATGGACT TTGAACGGGATGAGGAGCGCTCAGCCAACGGTGGCTCCGAATCTGACGGGGAGGAGAACATCGGCTGGAG CACGGTGAACCTGGACGAGGAGAAGCAGCAGCAGGATTTCTCTGCTTCCTCCACCACCATCCTGGACGAG GAACCGATCGTGAATAGGGGGCTGGCAGCTGCCCTGCTCCTGTGTCAGAACAAAGGGCTGCTGGAGACCA CAGTGCAGAAGGTGGCCCGGGTGAAGGCCCCCAACAAGTCGCTGCCCTCAGCCGTGTACTGCATCGAGGA TAAGATGGCCATCGATGACAAGTACAGCCGGAGGGAGGAATACCGAGGCTTCACACAGGACTTCAAGGAG AAGGACGGCTACAAACCCGACGTTAAGATCGAATACGTGGATGAGACGGGCCGGAAACTCACACCCAAGG AGGCTTTCCGGCAGCTGTCGCACCGCTTCCATGGCAAGGGCTCAGGCAAGATGAAGACAGAGCGGCGGAT GAAGAAGCTGGACGAGGAGGCGCTCCTGAAGAAGATGAGCTCCAGCGACACGCCCCTGGGCACCGTGGCC CTGCTCCAGGAGAAGCAGAAGGCTCAGAAGACCCCCTACATCGTGCTCAGCGGCAGCGGCAAGAGCATGA ACGCGAACACCATCACCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200603 representing NM_005146
Red=Cloning site Green=Tags(s) MGSSKKHRGEKEAAGTTAAAGTGGATEQPPRHREHKKHKHRSGGSGGSGGERRKRSRERGGERGSGRRGA EAEARSSTHGRERSQAEPSERRVKREKRDDGYEAAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAI KKEAGTKEEPVTADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQL QKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKG VLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFR LEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRA DDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVL EEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFC RTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDE EPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKE KDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVA LLQEKQKAQKTPYIVLSGSGKSMNANTITK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005146 |
ORF Size | 2400 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005146.2 |
RefSeq Size | 3601 bp |
RefSeq ORF | 2403 bp |
Locus ID | 9092 |
UniProt ID | O43290 |
Domains | SART-1 |
Protein Pathways | Spliceosome |
Gene Summary | This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200603 | SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) |
CNY 8,784.00 |
|
RC200603L1 | Lenti-ORF clone of SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) |
CNY 11,184.00 |
|
RC200603L2 | Lenti-ORF clone of SART1 (mGFP-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) |
CNY 8,740.00 |
|
RC200603L3 | Lenti-ORF clone of SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) |
CNY 8,740.00 |
|
RC200603L4 | Lenti-ORF clone of SART1 (mGFP-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) |
CNY 8,740.00 |
|
SC319408 | SART1 (untagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) |
CNY 8,800.00 |