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NCBP1 (NM_002486) Human Tagged ORF Clone
CAT＃: RG200630
- TrueORF®
NCBP1 (tGFP-tagged) - Human nuclear cap binding protein subunit 1, 80kDa (NCBP1)
ORF Plasmid: DDK
"NM_002486" in other vectors (6)
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CNY 7,410.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CBP80; NCBP; Sto1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG200630 representing NM_002486
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGGCGGCGGCACAGCGACGAGAACGACGGTGGGCAGCCTCACAAAAGGAGAAAGACCTCTGATG CAAATGAAACTGAAGATCATTTGGAATCTTTAATATGTAAAGTAGGAGAAAAGAGTGCCTGCTCTTTGGA GAGCAACCTAGAAGGCTTGGCTGGTGTTTTGGAAGCTGATCTTCCTAACTACAAGAGCAAGATCTTAAGG CTTCTTTGTACAGTTGCACGCCTATTACCTGAGAAGCTGACAATTTATACAACATTAGTTGGACTACTGA ATGCCAGGAATTACAATTTTGGTGGAGAATTTGTAGAAGCCATGATTCGTCAACTTAAAGAATCATTGAA AGCAAACAATTATAATGAAGCCGTGTATTTGGTCCGTTTTTTATCTGATCTTGTGAATTGTCATGTGATT GCCGCCCCATCAATGGTTGCTATGTTTGAAAATTTTGTAAGCGTAACTCAGGAAGAAGATGTACCTCAGG TGCGACGAGATTGGTATGTGTATGCATTTCTGTCATCTTTGCCCTGGGTTGGAAAGGAGTTGTACGAAAA GAAAGATGCAGAGATGGACCGCATCTTTGCCAACACTGAAAGCTATCTTAAAAGACGCCAAAAGACTCAT GTACCCATGTTACAGGTATGGACTGCTGATAAACCACATCCACAAGAAGAGTATTTAGATTGCCTGTGGG CCCAGATTCAGAAATTGAAAAAGGATCGCTGGCAGGAACGGCACATCCTAAGACCTTATCTTGCCTTTGA CAGCATCCTGTGTGAAGCACTGCAGCACAATCTGCCTCCTTTTACACCACCTCCTCACACTGAAGATTCA GTGTACCCAATGCCAAGGGTCATCTTCAGAATGTTTGATTACACAGATGATCCCGAGGGTCCTGTCATGC CAGGGAGTCATTCAGTGGAAAGATTTGTAATAGAAGAGAATCTTCACTGCATCATTAAGTCCCACTGGAA GGAAAGGAAGACTTGTGCTGCACAGTTAGTGAGCTATCCAGGGAAGAACAAGATCCCCTTGAACTACCAC ATAGTTGAGGTGATCTTTGCAGAGCTGTTTCAACTTCCAGCACCCCCTCACATTGATGTGATGTACACAA CACTCCTCATTGAACTGTGCAAACTTCAACCTGGCTCTCTACCCCAAGTTCTTGCACAGGCAACTGAAAT GCTATACATGCGTTTGGACACAATGAACACTACCTGTGTAGACAGGTTTATTAATTGGTTTTCTCATCAT CTAAGTAACTTCCAGTTCCGTTGGAGCTGGGAAGATTGGTCAGATTGTCTTAGTCAAGATCCTGAAAGTC CCAAACCGAAGTTTGTAAGAGAAGTTCTAGAAAAATGTATGAGGTTGTCTTACCATCAGCGTATATTAGA TATTGTTCCTCCTACCTTCTCAGCTCTGTGTCCTGCAAACCCAACCTGCATTTACAAGTATGGAGATGAA AGTAGCAATTCTCTTCCTGGACATTCTGTTGCCCTCTGTTTAGCTGTTGCCTTTAAAAGTAAGGCAACCA ATGATGAAATCTTCAGCATTCTGAAAGATGTACCAAATCCTAACCAGGATGATGACGACGATGAAGGATT CAGTTTTAACCCATTGAAAATAGAAGTCTTTGTACAGACTCTGCTACACTTGGCAGCCAAATCATTCAGC CACTCCTTCAGTGCTCTTGCAAAGTTTCATGAAGTCTTCAAAACCCTAGCTGAAAGTGATGAAGGAAAGT TACATGTGCTAAGAGTTATGTTTGAGGTCTGGAGGAACCATCCACAGATGATTGCTGTACTAGTGGATAA GATGATTCGTACACAAATAGTTGATTGTGCTGCCGTAGCAAATTGGATCTTCTCTTCAGAACTATCTCGT GACTTTACCAGATTGTTTGTTTGGGAAATTTTGCACTCTACAATTCGTAAGATGAACAAACATGTCCTGA AGATCCAGAAAGAGCTGGAAGAAGCTAAAGAGAAACTTGCTAGGCAACACAAACGGCGAAGTGATGATGA CGACAGAAGCAGTGACAGGAAAGACGGGGTTCTTGAGGAACAAATAGAACGACTTCAGGAAAAAGTGGAA TCTGCTCAGAGTGAACAAAAGAATCTTTTCCTCGTTATATTTCAGCGGTTTATCATGATCTTGACCGAGC ACCTAGTACGATGCGAAACTGATGGGACCAGTGTATTAACACCATGGTATAAGAACTGTATAGAGAGGCT GCAGCAGATCTTCCTACAGCATCACCAAATAATCCAGCAGTACATGGTGACCCTGGAGAACCTTCTCTTC ACTGCTGAATTAGACCCTCATATCTTGGCCGTGTTCCAGCAGTTCTGTGCCCTGCAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200630 representing NM_002486
Red=Cloning site Green=Tags(s) MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILR LLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVI AAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTH VPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDS VYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH IVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHH LSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDE SSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFS HSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSR DFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVE SAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLF TAELDPHILAVFQQFCALQA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002486 |
| ORF Size | 2370 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002486.5 |
| RefSeq Size | 5381 bp |
| RefSeq ORF | 2373 bp |
| Locus ID | 4686 |
| UniProt ID | Q09161 |
| Domains | MIF4G |
| Protein Pathways | Spliceosome |
| Gene Summary | The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200630 | NCBP1 (Myc-DDK-tagged)-Human nuclear cap binding protein subunit 1, 80kDa (NCBP1) |
CNY 5,784.00 |
|
| RC200630L1 | Lenti ORF clone of Human nuclear cap binding protein subunit 1, 80kDa (NCBP1), Myc-DDK-tagged |
CNY 8,184.00 |
|
| RC200630L2 | Lenti ORF clone of Human nuclear cap binding protein subunit 1, 80kDa (NCBP1), mGFP tagged |
CNY 8,650.00 |
|
| RC200630L3 | Lenti ORF clone of Human nuclear cap binding protein subunit 1, 80kDa (NCBP1), Myc-DDK-tagged |
CNY 8,650.00 |
|
| RC200630L4 | Lenti ORF clone of Human nuclear cap binding protein subunit 1, 80kDa (NCBP1), mGFP tagged |
CNY 8,184.00 |
|
| SC321555 | NCBP1 (untagged)-Human nuclear cap binding protein subunit 1, 80kDa (NCBP1) |
CNY 5,792.00 |
