RRM1 (NM_001033) Human Tagged ORF Clone
CAT#: RG200726
- TrueORF®
RRM1 (tGFP-tagged) - Human ribonucleotide reductase M1 (RRM1)
ORF Plasmid: DDK
"NM_001033" in other vectors (4)
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CNY 10,304.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | R1; RIR1; RR1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200726 representing NM_001033
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGTGATCAAGCGAGATGGCCGCCAAGAACGAGTCATGTTTGACAAAATTACATCTCGAATCCAGA AGCTTTGTTATGGACTCAATATGGATTTTGTTGATCCTGCTCAGATCACCATGAAAGTAATCCAAGGCTT GTACAGTGGGGTCACCACAGTGGAACTAGATACTTTGGCTGCTGAAACAGCTGCAACCTTGACTACTAAG CACCCTGACTATGCTATCCTGGCAGCCAGGATCGCTGTCTCTAACTTGCACAAAGAAACAAAGAAAGTGT TCAGTGATGTGATGGAAGACCTCTATAACTACATAAATCCACATAATGGCAAACACTCTCCCATGGTGGC CAAGTCAACATTGGATATTGTTCTGGCCAATAAAGATCGCCTGAATTCTGCTATTATCTATGACCGAGAT TTCTCTTACAATTACTTCGGCTTTAAGACGCTAGAGCGGTCTTATTTGTTGAAGATCAATGGAAAAGTGG CTGAAAGACCACAACATATGTTGATGAGAGTATCTGTTGGGATCCACAAAGAAGACATTGATGCAGCAAT TGAAACATATAATCTTCTTTCTGAGAGGTGGTTTACTCATGCTTCGCCCACTCTCTTCAATGCTGGTACC AACCGCCCACAACTTTCTAGCTGTTTTCTTCTGAGTATGAAAGATGACAGCATTGAAGGCATTTATGACA CTCTAAAGCAATGTGCATTGATTTCTAAGTCTGCTGGAGGAATTGGTGTTGCTGTGAGTTGTATTCGGGC TACTGGCAGCTACATTGCTGGGACTAATGGCAATTCCAATGGCCTTGTACCGATGCTGAGAGTATATAAC AACACAGCTAGATATGTGGATCAAGGTGGGAACAAGCGTCCTGGGGCATTTGCTATTTACCTGGAGCCTT GGCATTTAGACATCTTTGAATTCCTTGATTTAAAGAAGAACACAGGAAAGGAAGAGCAGCGTGCCAGAGA TCTTTTCTTTGCTCTTTGGATTCCGGATCTCTTCATGAAACGAGTGGAGACTAATCAGGACTGGTCTTTG ATGTGTCCAAATGAGTGTCCTGGTCTGGATGAGGTTTGGGGAGAGGAATTTGAGAAACTATATGCAAGTT ATGAGAAACAAGGTCGTGTCCGCAAAGTTGTAAAAGCTCAGCAGCTTTGGTATGCCATCATTGAGTCTCA GACGGAAACAGGCACCCCGTATATGCTCTACAAAGATTCCTGTAATCGAAAGAGCAACCAGCAGAACCTG GGAACCATCAAATGCAGCAACCTGTGCACAGAAATAGTGGAGTACACCAGCAAAGATGAGGTTGCTGTTT GTAATTTGGCTTCCCTGGCCCTGAATATGTATGTCACATCAGAACACACATACGACTTTAAGAAGTTGGC TGAAGTCACTAAAGTCGTTGTCCGAAACTTGAATAAAATTATTGATATAAACTACTATCCTGTACCAGAG GCATGCCTATCAAATAAACGCCATCGCCCCATTGGAATTGGGGTACAAGGTCTGGCAGATGCTTTTATCC TGATGAGATACCCTTTTGAGAGTGCAGAAGCCCAGTTACTGAATAAGCAGATCTTTGAAACTATTTATTA TGGTGCTCTGGAAGCCAGCTGTGACCTTGCCAAGGAGCAGGGCCCATACGAAACCTATGAGGGCTCTCCA GTTAGCAAAGGAATTCTTCAGTATGATATGTGGAATGTTACTCCTACAGACCTATGGGACTGGAAGGTTC TCAAGGAGAAGATTGCAAAGTATGGTATAAGAAACAGTTTACTTATTGCCCCGATGCCTACAGCTTCCAC TGCTCAGATCCTGGGGAATAATGAGTCCATTGAACCTTACACCAGCAACATCTATACTCGCAGAGTCTTG TCAGGAGAATTTCAGATTGTAAATCCTCACTTATTGAAAGATCTTACCGAGCGGGGCCTATGGCATGAAG AGATGAAAAACCAGATTATTGCATGCAATGGCTCTATTCAGAGCATACCAGAAATTCCTGATGACCTGAA GCAACTTTATAAAACTGTGTGGGAAATCTCTCAGAAAACTGTTCTCAAGATGGCAGCTGAGAGAGGTGCT TTCATTGATCAAAGCCAATCTTTGAACATCCACATTGCTGAGCCTAACTATGGCAAACTCACTAGTATGC ACTTCTACGGCTGGAAGCAGGGTTTGAAGACTGGGATGTATTATTTAAGGACGAGACCAGCAGCTAATCC AATCCAGTTCACTCTAAATAAGGAGAAGCTAAAAGATAAAGAAAAGGTATCAAAAGAGGAAGAAGAGAAG GAGAGGAACACAGCAGCCATGGTGTGCTCTTTGGAGAATAGAGATGAATGTCTGATGTGTGGATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200726 representing NM_001033
Red=Cloning site Green=Tags(s) MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVIQGLYSGVTTVELDTLAAETAATLTTK HPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYDRD FSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSERWFTHASPTLFNAGT NRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYN NTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWIPDLFMKRVETNQDWSL MCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVKAQQLWYAIIESQTETGTPYMLYKDSCNRKSNQQNL GTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYVTSEHTYDFKKLAEVTKVVVRNLNKIIDINYYPVPE ACLSNKRHRPIGIGVQGLADAFILMRYPFESAEAQLLNKQIFETIYYGALEASCDLAKEQGPYETYEGSP VSKGILQYDMWNVTPTDLWDWKVLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVL SGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGA FIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEK ERNTAAMVCSLENRDECLMCGS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033 |
ORF Size | 2376 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033.2, NP_001024.1 |
RefSeq Size | 3117 bp |
RefSeq ORF | 2379 bp |
Locus ID | 6240 |
UniProt ID | P23921 |
Domains | ribonuc_red_lg, ATP-cone |
Protein Families | Druggable Genome |
Protein Pathways | Glutathione metabolism, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
Gene Summary | This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200726 | RRM1 (Myc-DDK-tagged)-Human ribonucleotide reductase M1 (RRM1) |
CNY 8,704.00 |
|
RC200726L3 | Lenti ORF clone of Human ribonucleotide reductase M1 (RRM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200726L4 | Lenti ORF clone of Human ribonucleotide reductase M1 (RRM1), mGFP tagged |
CNY 11,104.00 |
|
SC111007 | RRM1 (untagged)-Human ribonucleotide reductase M1 (RRM1) |
CNY 8,712.00 |