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C3IP1 (KLHL12) (NM_021633) Human Tagged ORF Clone
CAT＃: RG200894
- TrueORF®
KLHL12 (tGFP-tagged) - Human kelch-like 12 (Drosophila) (KLHL12)
ORF Plasmid: DDK
"NM_021633" in other vectors (6)
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CNY 7,944.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C3IP1; DKIR |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG200894 representing NM_021633
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCAATGA ACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAAGACTTCCCTGCCCA TCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTCACTAGTGAGCTCTCAGAGAAGGGG AAACCTTATGTTGACATCCAAGGTTTGACTGCCTCTACCATGGAAATTTTATTGGACTTTGTGTACACAG AAACAGTACATGTGACAGTGGAGAATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGG TGTGAAACAAGCCTGCTGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGAT TTTGCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTTCCTG AAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTAATCAAGTGCGACGA AATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAACTGGGTGAAGCATGCCAAGAAAGAG CGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTGCGGATGCCCCTACTAACCCCCAGGTATATCACAG ATGTAATAGATGCTGAGCCTTTCATCCGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAA GTTTCATCTGAGGCCTGAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAAT GAAGTGCTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATATGACC CCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTGGCCTCAGTGTCCCT TCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGCCTTAGTTCAGTGGAATGTCTAGAC TACACAGCAGATGAGGATGGGGTCTGGTATTCTGTGGCCCCTATGAATGTCCGACGAGGTCTTGCTGGAG CCACCACCCTGGGAGATATGATCTATGTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGA GCGCTATGATCCAAACATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGA CTCGTAGTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCAGTTG AGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAGCGTTCTGGTGCAGG AGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGATGGTACAGCCCACCTTTCTTCCGTT GAAGCATACAACATTCGCACTGATTCCTGGACAACTGTCACCAGTATGACCACTCCACGATGCTATGTAG GGGCCACAGTGCTTCGGGGGAGACTCTATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCAT TGAATGTTATGACCCTATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCT GGTGTTTGTGTTCTCCGCGAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200894 representing NM_021633
Red=Cloning site Green=Tags(s) MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKG KPYVDIQGLTASTMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRD FAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKE REESLPNLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGAN EVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLD YTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAG LVVASGVIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSV EAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVTSMGTQRCDA GVCVLREK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021633 |
| ORF Size | 1704 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021633.4 |
| RefSeq Size | 3338 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 59349 |
| UniProt ID | Q53G59 |
| Domains | BTB, Kelch |
| Gene Summary | This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200894 | KLHL12 (Myc-DDK-tagged)-Human kelch-like 12 (Drosophila) (KLHL12) |
CNY 6,344.00 |
|
| RC200894L1 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), Myc-DDK-tagged |
CNY 8,744.00 |
|
| RC200894L2 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), mGFP tagged |
CNY 5,890.00 |
|
| RC200894L3 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200894L4 | Lenti ORF clone of Human kelch-like 12 (Drosophila) (KLHL12), mGFP tagged |
CNY 8,744.00 |
|
| SC112907 | KLHL12 (untagged)-Human kelch-like 12 (Drosophila) (KLHL12) |
CNY 6,352.00 |
