SAR1 (SAR1A) (NM_020150) Human Tagged ORF Clone
CAT#: RG201450
- TrueORF®
SAR1A (tGFP-tagged) - Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2
ORF Plasmid: DDK
"NM_020150" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | masra2; SAR1; Sara; SARA1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201450 representing NM_020150
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTCATCTTTGAGTGGATCTACAATGGCTTCAGCAGTGTGCTCCAGTTCCTAGGACTGTACAAGA AATCTGGAAAACTTGTATTCTTAGGTTTGGATAATGCAGGCAAAACCACTCTTCTTCACATGCTCAAAGA TGACAGATTGGGCCAACATGTTCCAACACTACATCCGACATCAGAAGAGCTAACAATTGCTGGAATGACC TTTACAACTTTTGATCTTGGTGGGCACGAGCAAGCACGTCGCGTTTGGAAAAATTATCTCCCAGCAATTA ATGGGATTGTCTTTCTGGTGGACTGTGCAGATCATTCTCGCCTCGTGGAATCCAAAGTTGAGCTTAATGC TTTAATGACTGATGAAACAATATCCAATGTGCCAATCCTTATCTTGGGTAACAAAATTGACAGAACAGAT GCAATCAGTGAAGAAAAACTCCGTGAGATATTTGGGCTTTATGGACAGACCACAGGAAAGGGGAATGTGA CCCTGAAGGAGCTGAATGCTCGCCCCATGGAAGTGTTCATGTGCAGTGTGCTCAAGAGGCAAGGTTACGG CGAGGGTTTCCGCTGGCTCTCCCAGTATATTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201450 representing NM_020150
Red=Cloning site Green=Tags(s) MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMT FTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTD AISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020150 |
ORF Size | 594 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020150.5 |
RefSeq Size | 761 bp |
RefSeq ORF | 597 bp |
Locus ID | 56681 |
UniProt ID | Q9NR31 |
Domains | SAR, ARF, arf |
Gene Summary | Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201450 | SAR1A (Myc-DDK-tagged)-Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2 |
CNY 2,400.00 |
|
RC201450L1 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201450L2 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC201450L3 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201450L4 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC113196 | SAR1A (untagged)-Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2 |
CNY 2,400.00 |