THEM2 (ACOT13) (NM_018473) Human Tagged ORF Clone

CAT#: RG201505

  • TrueORF®

ACOT13 (tGFP-tagged) - Human acyl-CoA thioesterase 13 (ACOT13), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_018473" in other vectors (6)

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CNY 2,800.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal THEM2 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms HT012; PNAS-27; THEM2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG201505 representing NM_018473
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCAGCATGACTCAGTCTCTGCGGGAGGTGATAAAGGCCATGACCAAGGCTCGCAATTTTGAGAGAG
TTTTGGGAAAGATTACTCTTGTCTCTGCTGCTCCTGGGAAAGTGATTTGTGAAATGAAAGTAGAAGAAGA
GCATACCAATGCAATAGGCACTCTCCACGGCGGTTTGACAGCCACGTTAGTAGATAACATATCAACAATG
GCTCTGCTATGCACGGAAAGGGGAGCACCCGGAGTCAGTGTCGATATGAACATAACGTACATGTCACCTG
CAAAATTAGGAGAAGATATAGTGATTACAGCACATGTTCTGAAGCAAGGAAAAACACTTGCATTTACCTC
TGTGGATCTGACCAACAAGGCCACAGGAAAATTAATAGCACAAGGAAGACACACAAAACACCTGGGAAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG201505 representing NM_018473
Red=Cloning site Green=Tags(s)

MTSMTQSLREVIKAMTKARNFERVLGKITLVSAAPGKVICEMKVEEEHTNAIGTLHGGLTATLVDNISTM
ALLCTERGAPGVSVDMNITYMSPAKLGEDIVITAHVLKQGKTLAFTSVDLTNKATGKLIAQGRHTKHLGN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018473
ORF Size 420 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018473.4
RefSeq Size 753 bp
RefSeq ORF 423 bp
Locus ID 55856
UniProt ID Q9NPJ3
Domains 4HBT
Gene Summary This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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