United States
Germany
Japan
United Kingdom
China
ACTR1B (NM_005735) Human Tagged ORF Clone
CAT＃: RG202493
- TrueORF®
ACTR1B (tGFP-tagged) - Human ARP1 actin-related protein 1 homolog B, centractin beta (yeast) (ACTR1B)
ORF Plasmid: DDK
"NM_005735" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 5,256.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARP1B; CTRN2; PC3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202493 representing NM_005735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTGATTAAAGCTG GCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGGCGGCCGAAGCACATGCGGGT GATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCAAAAGCAGAGGAGCACCGGGGGCTGCTGACC ATCCGCTACCCCATGGAGCACGGCGTGGTGCGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCT ACTCCAAGGATCAGCTGCAGACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCC GAGTAAGAACCGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCAGGGGACGGGG TCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATCATGCGGGTGGACATTGCCGG CCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAGGAAGGGGTTGACTTCCATACCTCGGCTGAG TTTGAGGTTGTCCGGACAATCAAAGAGCGAGCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTC TGGAGACGGAGAAGGTGCAGTACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCG GGCCCCCGAGCTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTCTCAGGTGGCT CAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAGCTTGCCCCAAAGGATATCAA AATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACATGGATTGGCGGCTCCATCCTGGCCTCGCTG GACACTTTTAAGAAGATGTGGGTGTCCAAAAAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCA AAACTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202493 representing NM_005735
Red=Cloning site Green=Tags(s) MESYDIIANQPVVIDNGSGVIKAGFAGDQIPKYCFPNYVGRPKHMRVMAGALEGDLFIGPKAEEHRGLLT IRYPMEHGVVRDWNDMERIWQYVYSKDQLQTFSEEHPVLLTEAPLNPSKNREKAAEVFFETFNVPALFIS MQAVLSLYATGRTTGVVLDSGDGVTHAVPIYEGFAMPHSIMRVDIAGRDVSRYLRLLLRKEGVDFHTSAE FEVVRTIKERACYLSINPQKDEALETEKVQYTLPDGSTLDVGPARFRAPELLFQPDLVGDESEGLHEVVA FAIHKSDMDLRRTLFANIVLSGGSTLFKGFGDRLLSEVKKLAPKDIKIKISAPQERLYSTWIGGSILASL DTFKKMWVSKKEYEEDGSRAIHRKTF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005735 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005735.2, NP_005726.1 |
| RefSeq Size | 2054 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 10120 |
| UniProt ID | P42025 |
| Domains | ACTIN |
| Gene Summary | This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202493 | ACTR1B (Myc-DDK-tagged)-Human ARP1 actin-related protein 1 homolog B, centractin beta (yeast) (ACTR1B) |
CNY 3,656.00 |
|
| RC202493L3 | Lenti ORF clone of Human ARP1 actin-related protein 1 homolog B, centractin beta (yeast) (ACTR1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202493L4 | Lenti ORF clone of Human ARP1 actin-related protein 1 homolog B, centractin beta (yeast) (ACTR1B), mGFP tagged |
CNY 5,890.00 |
|
| SC116594 | ACTR1B (untagged)-Human ARP1 actin-related protein 1 homolog B, centractin beta (yeast) (ACTR1B) |
CNY 3,656.00 |
|
| SC320716 | ACTR1B (untagged)-Human ARP1 actin-related protein 1 homolog B, centractin beta (yeast) (ACTR1B) |
CNY 3,656.00 |
