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C12orf32 (NM_031465) Human Tagged ORF Clone
CAT＃: RG203020
- TrueORF®
C12orf32 (tGFP-tagged) - Human chromosome 12 open reading frame 32 (C12orf32)
ORF Plasmid: DDK
"NM_031465" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | chromosome 12 open reading frame 32; HKMT1188; HKMT1188, MGC13204; MGC13204 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203020 representing NM_031465
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCCAGAAAAAAACGCCGCCAGCCTTCCCAGAAAGCCCCGCTGCTGTTCCACCAACAACCACTGG AGGGCCCCAAACACAGCTGTGCATCTACACAGCTTCCCATCACTCACACTCGACAGGTGCCCAGCAAGCC CATTGACCACAGCACCATCACTTCCTGGGTATCACCTGATTTTGATACAGCAGCAGGAAGCTTGTTCCCA GCCTACCAGAAACACCAAAACCGGGCGAGACACTCAAGTCGAAAACCTACCACCTCCAAGTTTCCACATC TAACTTTTGAGAGTCCGCAATCTTCCAGTTCAGAGACATTGGGGATCCCCTTAATCCGAGAGTGCCCCAG TGAATCAGAAAAGGATGTTTCCAGAAGACCCTTAGTTCCAGTGCTCAGTCCCCAAAGCTGTGGGAACATG TCAGTGCAGGCACTTCAGAGCTTACCTTATGTGTTCATTCCACCTGATATCCAGACCCCAGAGTCATCAT CTGTGAAGGAAGAACTCATTCCCCAAGATCAGAAGGAAAACAGCCTTCTAAGCTGCACTCTTCACACTGG CACTCCTAATAGCCCAGAGCCTGGACCTGTTCTGGTTAAAGACACCCCCGAGGACAAGTATGGAATAAAG GTCACATGGAGGAGACGACAGCACCTGCTTGCTTACCTCAGGGAGAGAGGGAAGCTGAGCAGAAGCCAAT TCCTTGTGAAAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203020 representing NM_031465
Red=Cloning site Green=Tags(s) MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFP AYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNM SVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIK VTWRRRQHLLAYLRERGKLSRSQFLVKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031465 |
| ORF Size | 714 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031465.2, NP_113653.1 |
| RefSeq Size | 1872 bp |
| RefSeq ORF | 716 bp |
| Locus ID | 83695 |
| Gene Summary | Plays a role in DNA damage response (DDR) signaling upon genotoxic stresses such as ionizing radiation (IR) during the S phase. Recruited to sites of DNA damage through interaction with the 9-1-1 cell-cycle checkpoint response complex and TOPBP1 in a ATR-dependent manner. Required for the progression of the G1 to S phase transition. Plays a role in the stimulation of CHEK1 phosphorylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203020 | C12orf32 (Myc-DDK-tagged)-Human chromosome 12 open reading frame 32 (C12orf32) |
CNY 2,400.00 |
|
| RC203020L1 | Lenti ORF clone of Human chromosome 12 open reading frame 32 (C12orf32), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203020L2 | Lenti ORF clone of Human chromosome 12 open reading frame 32 (C12orf32), mGFP tagged |
CNY 5,890.00 |
|
| RC203020L3 | Lenti ORF clone of Human chromosome 12 open reading frame 32 (C12orf32), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203020L4 | Lenti ORF clone of Human chromosome 12 open reading frame 32 (C12orf32), mGFP tagged |
CNY 5,890.00 |
|
| SC108000 | C12orf32 (untagged)-Human chromosome 12 open reading frame 32 (C12orf32) |
CNY 2,400.00 |
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