NDUFS7 (NM_024407) Human Tagged ORF Clone
CAT#: RG203279
- TrueORF®
NDUFS7 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase) (NDUFS7), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_024407" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CI-20; CI-20KD; MC1DN3; MY017; PSST |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203279 representing NM_024407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGCTGTCAGCTCCTGGCCTGCGCGGCTTCCGGATCCTTGGTCTGCGCTCCAGCGTGGGCCTGG CTGTGCAGGCACGAGGTGTCCATCAGAGCGTGGCCACCGATGGCCCAAGCAGCACCCAGCCTGCCCTGCC AAAGGCCAGAGCCGTGGCTCCCAAACCCAGCAGCCGGGGCGAGTATGTGGTGGCCAAGCTGGATGACCTC GTCAACTGGGCCCGCCGGAGTTCTCTGTGGCCCATGACCTTCGGCCTGGCCTGCTGCGCCGTGGAGATGA TGCACATGGCAGCACCCCGCTACGACATGGACCGCTTTGGCGTGGTCTTCCGCGCCAGCCCGCGCCAGTC CGACGTCATGATCGTGGCCGGCACACTCACCAACAAGATGGCCCCAGCGCTTCGCAAGGTCTACGACCAG ATGCCGGAGCCGCGCTACGTGGTCTCCATGGGGAGCTGCGCCAACGGAGGAGGCTACTACCACTATTCCT ACTCGGTGGTGAGGGGCTGCGACCGCATCGTGCCCGTGGACATCTACATCCCAGGCTGCCCACCTACGGC CGAGGCCCTGCTCTACGGCATCCTGCAGCTGCAGAGGAAGATCAAGCGGGAGCGGAGGCTGCAGATCTGG TACCGCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203279 representing NM_024407
Red=Cloning site Green=Tags(s) MAVLSAPGLRGFRILGLRSSVGLAVQARGVHQSVATDGPSSTQPALPKARAVAPKPSSRGEYVVAKLDDL VNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKVYDQ MPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYIPGCPPTAEALLYGILQLQRKIKRERRLQIW YRR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024407 |
ORF Size | 639 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024407.3, NP_077718.2 |
RefSeq Size | 787 bp |
RefSeq ORF | 642 bp |
Locus ID | 374291 |
UniProt ID | O75251 |
Domains | oxidored_q6 |
Protein Pathways | Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease |
Gene Summary | This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203279 | NDUFS7 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase) (NDUFS7), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
|
RC203279L3 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase) (NDUFS7), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203279L4 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase) (NDUFS7), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
SC108125 | NDUFS7 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase) (NDUFS7), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |