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RNMTL1 (MRM3) (NM_018146) Human Tagged ORF Clone
CAT＃: RG204113
- TrueORF®
RNMTL1 (tGFP-tagged) - Human RNA methyltransferase like 1 (RNMTL1)
ORF Plasmid: DDK
"NM_018146" in other vectors (4)
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CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RMTL1; RNMTL1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204113 representing NM_018146
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGGTGAGACCCTCGAGGTTTGTCGTGCGACCGTTGCTGCAGGTGGTCCAGGCTTGGGACC TTGACGCGAGGCGCTGGGTCCGGGCGCTGCGGCGGAGCCCAGTGAAAGTGGTGTTTCCTTCCGGAGAGGT GGTGGAACAGAAGCGCGCTCCTGGGAAGCAGCCCCGCAAGGCACCATCTGAGGCCAGTGCCCAGGAGCAA CGAGAGAAACAACCGCTCGAGGAGTCCGCATCCCGCGCTCCCAGCACCTGGGAAGAGTCTGGGCTTCGCT ACGATAAAGCTTATCCCGGGGACAGGAGGCTGAGCAGTGTAATGACAATAGTAAAGTCCAGGCCATTTCG GGAAAAACAAGGGAAGATCCTGCTGGAAGGTCGCAGGCTCATTTCAGACGCTCTCAAGGCTGGAGCTGTG CCAAAAATGTTCTTCTTTAGCCGTCTAGAATACCTAAAGGAGTTGCCAGTCGATAAGCTGAAAGGTGTCA GCCTCATTAAGGTGAAATTTGAGGATATCAAGGATTGGTCCGACCTCGTAACGCCACAAGGAATAATGGG GATTTTTGCCAAGCCTGACCATGTTAAGATGACATATCCAAAGACTCAGCTTCAGCATTCACTGCCTTTA TTATTGATTTGTGACAATCTCCGTGACCCTGGGAACCTGGGGACAATTCTGAGATCTGCAGCTGGGGCAG GCTGCAGCAAAGTGTTACTCACCAAAGGCTGTGTGGATGCCTGGGAGCCCAAAGTGCTCCGGGCGGGTAT GGGCGCACATTTCCGGATGCCCATTATCAATAATCTGGAATGGGAAACCGTGCCCAATTACCTGCCCCCT GACACTCGGGTCTATGTGGCTGACAACTGTGGCCTTTATGCCCAGGCTGAGATGTCTAATAAAGCTAGTG ACCATGGCTGGGTGTGTGATCAACGAGTGATGAAGTTTCACAAGTATGAGGAAGAGGAAGATGTAGAAAC CGGAGCCAGTCAAGATTGGCTGCCTCATGTTGAGGTTCAGAGTTACGACTCGGACTGGACAGAGGCGCCG GCAGCTGTGGTGATTGGCGGGGAGACCTACGGCGTGAGCCTGGAGTCCCTGCAGCTGGCCGAGAGCACTG GTGGCAAGAGGCTGCTGATCCCCGTTGTGCCTGGTGTGGACAGCCTCAACTCGGCCATGGCGGCAAGCAT CCTGCTTTTCGAAGGGAAAAGACAGCTGCGGGGGAGGGCGGAGGACTTGAGCAGGGACAGGAGTTACCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204113 representing NM_018146
Red=Cloning site Green=Tags(s) MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQ REKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAV PKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGIMGIFAKPDHVKMTYPKTQLQHSLPL LLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPP DTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAP AAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018146 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018146.2, NP_060616.1 |
| RefSeq Size | 1815 bp |
| RefSeq ORF | 1263 bp |
| Locus ID | 55178 |
| UniProt ID | Q9HC36 |
| Domains | SpoU_methylase |
| Protein Families | Stem cell - Pluripotency |
| Gene Summary | Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204113 | RNMTL1 (Myc-DDK-tagged)-Human RNA methyltransferase like 1 (RNMTL1) |
CNY 3,656.00 |
|
| RC204113L3 | Lenti ORF clone of Human RNA methyltransferase like 1 (RNMTL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204113L4 | Lenti ORF clone of Human RNA methyltransferase like 1 (RNMTL1), mGFP tagged |
CNY 5,890.00 |
|
| SC113706 | RNMTL1 (untagged)-Human RNA methyltransferase like 1 (RNMTL1) |
CNY 7,220.00 |
