United States
Germany
Japan
United Kingdom
China
EID2 (NM_153232) Human Tagged ORF Clone
CAT＃: RG204273
- TrueORF®
EID2 (tGFP-tagged) - Human EP300 interacting inhibitor of differentiation 2 (EID2)
ORF Plasmid: DDK
"NM_153232" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CRI2; EID-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204273 representing NM_153232
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAGCTGCCCGCAGACAGCAGTGTCCCGCAGACAGGCGCGGCGAATGGTGACAGAGACGTCCCGC AGGCGGAGGTAGGCCGCGGGAGGCGGGAGCCGGCCCCGGCACAGCCTGAGGAGGCCGGGGAAGGCGCGAT GGCGGCGGCCAGGGGAGGCCCGGTGCCGGCGGCCAGGGAAGGTCGGATGGCGGCGGCCAGGGCAGCCCCG GCGGCGGCAGCCAGGGGAGCCCCGGTGGCAGCGGCGGCGTTGGCCAGGGCAGCCGCGGCGGGCAGGGAAA GCCCGGCGGCGGCGGCGGCCAGGGAAGCCCGGATGGCGGAGGTCGCCCGCTTGCTGGGGGAGCCAGTGGA CGAAGAGGGTCCCGAGGGCAGGCCCAGGTCCAGACACGGGAACGGAGGCCTGGCTGCGTTGCCCTATCTC CGTCTCCGCCACCCACTTAGCGTTTTAGGCATCAATTACCAGCAGTTTCTCCGCCACTATCTGGAAAATT ACCCGATTGCTCCCGGCAGAATACAAGAGCTTGAAGAACGCCGCAGGCGATTCGTGGAAGCCTGCAGAGC AAGGGAAGCAGCGTTTGATGCCGAATATCAGCGAAATCCTCACAGGGTGGACCTCGATATTTTAACCTTT ACGATAGCTCTGACTGCCTCTGAAGTTATCAACCCTCTGATAGAAGAACTTGGTTGCGATAAGTTTATCA ATAGAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204273 representing NM_153232
Red=Cloning site Green=Tags(s) MSKLPADSSVPQTGAANGDRDVPQAEVGRGRREPAPAQPEEAGEGAMAAARGGPVPAAREGRMAAARAAP AAAARGAPVAAAALARAAAAGRESPAAAAAREARMAEVARLLGEPVDEEGPEGRPRSRHGNGGLAALPYL RLRHPLSVLGINYQQFLRHYLENYPIAPGRIQELEERRRRFVEACRAREAAFDAEYQRNPHRVDLDILTF TIALTASEVINPLIEELGCDKFINRE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153232 |
| ORF Size | 708 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153232.4 |
| RefSeq Size | 1409 bp |
| RefSeq ORF | 711 bp |
| Locus ID | 163126 |
| UniProt ID | Q8N6I1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
