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USP49 (NM_018561) Human Tagged ORF Clone
CAT＃: RG204345
- TrueORF®
USP49 (tGFP-tagged) - Human ubiquitin specific peptidase 49 (USP49)
ORF Plasmid: DDK
"NM_018561" in other vectors (6)
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CNY 6,368.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204345 representing NM_018561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGACCACTCCATCCTGAACCCTCAGAAGT GGTGCTGCTTAGAGTGTGCCACCACCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGG CCGCTATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCTAGCCATGGAAGTCCGG GATCTCTACGTGTTCTGTTACCTGTGCAAGGACTACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGC TGCTAAGAAGCTCCCTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCGGACGCT GCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCGCTCCTCAGGGACAGCCGCAGATGCTC ACGGCTCTGTGGTACCGGCGTCAGCGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCC GGGGCCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGAAGGAGGAGGCGCGGAG GCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAGGAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGG CTGCTCCTGCACACGCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCTCACGCA GAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGGCCTGCGCAA CCTGGGCAACACCTGCTACATGAACTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGT TTCCTCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTT CTGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCG GGAGGGCTTCTGCTGGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCG AGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCGGGAAGTGGG CCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCA ACAGGACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACC ACACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCA TATTTCATGGGCAGCTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCATTGAGCC CTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTCCCTTTGAAT CAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCT ACGCTTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAG AAAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGC CGTAATCATCGAGAGAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCT GCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGTCATGCATCA CGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCTACAACACAGAGGGAGGTGCGTGCGCTTTA CTCTGTGGGGTGGGGGACACGGAAAGGGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204345 representing NM_018561
Red=Cloning site Green=Tags(s) MDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVR DLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQML TALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSAR LLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFREC FLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEP SSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGT TRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLN QTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSG RNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACAL LCGVGDTERG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018561 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018561.3, NP_061031.2 |
| RefSeq Size | 3194 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 25862 |
| UniProt ID | Q70CQ1 |
| Domains | UCH, zf-UBP |
| Protein Families | Protease, Transcription Factors |
| Gene Summary | Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204345 | USP49 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 49 (USP49) |
CNY 4,768.00 |
|
| RC204345L1 | Lenti ORF clone of Human ubiquitin specific peptidase 49 (USP49), Myc-DDK-tagged |
CNY 7,168.00 |
|
| RC204345L2 | Lenti ORF clone of Human ubiquitin specific peptidase 49 (USP49), mGFP tagged |
CNY 6,460.00 |
|
| RC204345L3 | Lenti ORF clone of Human ubiquitin specific peptidase 49 (USP49), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204345L4 | Lenti ORF clone of Human ubiquitin specific peptidase 49 (USP49), mGFP tagged |
CNY 6,460.00 |
|
| SC113428 | USP49 (untagged)-Human ubiquitin specific peptidase 49 (USP49) |
CNY 4,776.00 |
