KIFAP3 (NM_014970) Human Tagged ORF Clone
CAT#: RG205208
- TrueORF®
KIFAP3 (tGFP-tagged) - Human kinesin-associated protein 3 (KIFAP3), transcript variant 1
ORF Plasmid: DDK
"NM_014970" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; SMAP; Smg-GDS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205208 representing NM_014970
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGGGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGAGGGAATATAGATGTACATCCATCAG AAAAAGCACTCATTGTTCACTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGGGACCCCATGTTGGG AGAACGAAAAGAATGTCAAAAAATCATTCGACTTAAGAGTCTCAATGCCAACACAGATATAACTTCCCTG GCAAGGAAGGTGGTTGAAGAATGTAAACTCATTCATCCTTCAAAACTAAATGAGGTAGAACAGCTGTTGT ACTATCTACAGAACCGCCGTGATTCATTGTCAGGAAAAGAGAAAAAAGAAAAATCAAGCAAGCCTAAAGA TCCACCTCCTTTTGAAGGAATGGAGATTGATGAAGTTGCTAACATTAATGACATGGATGAATATATTGAG TTATTATATGAAGATATTCCTGACAAAGTTCGGGGTTCTGCTTTGATCCTGCAGCTTGCTCGAAATCCTG ATAACTTGGAAGAACTACTATTGAATGAAACTGCCCTTGGTGCATTAGCAAGGGTCCTGAGAGAAGACTG GAAGCAAAGTGTCGAGTTAGCTACAAACATAATTTACATCTTTTTTTGTTTCTCCAGCTTTTCTCAATTT CATGGACTTATTACTCACTATAAAATTGGAGCTCTGTGTATGAATATTATTGATCATGAGTTAAAAAGAC ATGAGCTTTGGCAAGAAGAACTCTCAAAGAAGAAGAAAGCTGTTGATGAAGACCCTGAAAACCAAACCTT GAGAAAGGATTATGAAAAAACCTTTAAAAAGTACCAGGGGCTTGTGGTAAAACAGGAACAGCTATTACGA GTTGCTCTTTATTTGCTTCTGAATCTTGCTGAGGATACTCGTACCGAACTGAAAATGAGGAACAAGAACA TAGTTCACATGTTGGTGAAAGCCCTTGATCGGGACAATTTTGAGCTGCTAATTTTAGTTGTGTCATTCTT GAAGAAACTCAGCATTTTTATGGAGAATAAAAATGATATGGTGGAAATGGATATTGTTGAAAAACTGGTG AAAATGATACCTTGTGAGCATGAAGACCTGCTGAATATCACCCTCCGACTTTTACTAAACCTATCCTTTG ACACAGGACTGAGGAATAAGATGGTACAAGTTGGACTGCTTCCCAAGCTCACTGCACTCCTAGGCAATGA CAACTACAAACAAATAGCAATGTGTGTTCTTTACCACATAAGCATGGATGACCGCTTTAAATCAATGTTT GCATACACTGACTGTATACCACAGTTAATGAAGATGCTGTTTGAATGTTCAGATGAACGAATTGACTTGG AACTCATTTCTTTCTGCATTAATCTTGCTGCTAACAAAAGAAATGTACAGCTTATCTGTGAAGGAAATGG GCTGAAGATGCTCATGAAGAGGGCTCTGAAGTTTAAGGATCCATTGCTGATGAAAATGATTAGAAACATT TCTCAGCATGATGGACCAACTAAAAATCTGTTTATTGATTATGTTGGGGACCTTGCAGCCCAGATCTCTA ATGATGAAGAAGAGGAGTTTGTGATTGAATGTTTGGGAACTCTTGCAAACTTGACCATTCCAGACTTAGA CTGGGAATTGGTTCTTAAAGAATATAAGTTGGTTCCATACCTCAAGGATAAACTAAAACCAGGTGCTGCA GAAGATGATCTTGTTTTAGAAGTGGTTATAATGATTGGAACTGTATCCATGGATGACTCTTGTGCTGCAT TGCTAGCCAAATCTGGCATAATCCCTGCACTCATTGAATTGCTAAATGCTCAACAAGAAGATGATGAATT TGTGTGTCAGATAATTTATGTCTTCTACCAGATGGTTTTCCACCAAGCCACAAGAGACGTCATAATCAAG GAAACACAGGCTCCAGCATATCTCATAGACCTAATGCATGATAAGAATAATGAAATCCGAAAGGTCTGTG ATAATACATTAGATATTATAGCGGAATATGATGAAGAATGGGCTAAGAAAATTCAGAGTGAAAAGTTTCG CTGGCATAACTCTCAGTGGCTGGAGATGGTAGAGAGTCGTCAGATGGATGAGAGTGAGCAGTACTTGTAT GGTGATGATCGAATTGAGCCATACATTCATGAAGGAGATATTCTCGAAAGACCTGACCTTTTCTACAACT CAGATGGATTAATTGCCTCTGAAGGAGCCATAAGTCCCGATTTCTTCAATGATTACCACCTTCAAAATGG AGATGTTGTTGGGCAGCATTCATTTCCTGGCAGCCTTGGAATGGATGGCTTTGGCCAACCAGTTGGCATT CTTGGACGCCCTGCCACAGCATATGGATTCCGCCCTGATGAACCTTACTACTATGGCTATGGATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205208 representing NM_014970
Red=Cloning site Green=Tags(s) MQGEDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSL ARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIE LLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQF HGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLR VALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLV KMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMF AYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNI SQHDGPTKNLFIDYVGDLAAQISNDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAA EDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIK ETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQMDESEQYLY GDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGI LGRPATAYGFRPDEPYYYGYGS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_014970 |
ORF Size | 2376 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014970.4 |
RefSeq Size | 3011 bp |
RefSeq ORF | 2379 bp |
Locus ID | 22920 |
UniProt ID | Q92845 |
Domains | Armadillo_seg |
Protein Families | Druggable Genome |
Gene Summary | The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205208 | KIFAP3 (Myc-DDK-tagged)-Human kinesin-associated protein 3 (KIFAP3), transcript variant 1 |
CNY 8,704.00 |
|
RC205208L3 | Lenti-ORF clone of KIFAP3 (Myc-DDK-tagged)-Human kinesin-associated protein 3 (KIFAP3), transcript variant 1 |
CNY 5,890.00 |
|
RC205208L4 | Lenti-ORF clone of KIFAP3 (mGFP-tagged)-Human kinesin-associated protein 3 (KIFAP3), transcript variant 1 |
CNY 5,890.00 |
|
SC126979 | KIFAP3 (untagged)-Human kinesin-associated protein 3 (KIFAP3), transcript variant 1 |
CNY 9,288.00 |