GPR135 (NM_022571) Human Tagged ORF Clone
CAT#: RG205463
- TrueORF®
GPR135 (tGFP-tagged) - Human G protein-coupled receptor 135 (GPR135)
ORF Plasmid: DDK
"NM_022571" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HUMNPIIY20 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205463 representing NM_022571
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCCGCAGCCGCCCCGCCCACCAGCGAGCATGGCCTTACTGGGCAGCCAGCACTCCGGCGCCC CCTCCGCGGCCGGCCCACCTGGCGGGACTTCCTCCGCGGCCACGGCGGCCGTGCTCTCCTTCAGCACCGT GGCGACCGCGGCGCTGGGGAACCTGAGCGACGCAAGCGGAGGCGGCACAGCTGCCGCTCCCGGTGGCGGC GGCCTTGGCGGGTCCGGGGCAGCGCGGGAGGCGGGGGCGGCGGTGAGGCGGCCGCTAGGCCCGGAGGCGG CGCCGCTGCTGTCGCACGGAGCTGCAGTGGCGGCCCAGGCGCTCGTCCTCCTGCTCATCTTCCTGCTGTC TAGCCTTGGCAACTGCGCGGTGATGGGGGTGATTGTGAAGCACCGGCAGCTCCGCACCGTCACCAACGCC TTCATCCTGTCGCTGTCCCTATCGGATCTGCTCACGGCGCTGCTCTGCCTGCCCGCCGCCTTCCTGGACC TCTTCACTCCGCCCGGGGGTTCGGCGCCTGCCGCCGCCGCGGGGCCCTGGCGCGGCTTCTGCGCCGCCAG CCGCTTCTTCAGCTCGTGCTTCGGCATCGTGTCCACGCTCAGCGTGGCGCTCATCTCGTTGGACCGTTAC TGCGCTATCGTGCGGCCGCCGCGGGAGAAGATCGGCCGCCGCCGCGCGCTGCAGCTGCTGGCGGGCGCCT GGCTGACGGCCCTGGGCTTCTCCTTGCCCTGGGAGCTGCTCGGGGCGCCCCGGGAACTCGCGGCGGCGCA GAGCTTCCACGGCTGCCTCTACCGGACCTCCCCGGACCCCGCGCAGCTGGGCGCGGCCTTCAGCGTGGGG CTGGTGGTGGCCTGCTACCTGCTGCCCTTCCTGCTCATGTGCTTCTGCCACTACCACATCTGCAAGACGG TGCGCCTGTCGGACGTGCGCGTGCGGCCGGTGAACACCTACGCGCGCGTGCTGCGCTTCTTCAGCGAGGT GCGCACGGCCACCACCGTCCTCATCATGATCGTCTTCGTCATCTGCTGCTGGGGGCCCTACTGCTTCCTG GTGCTGCTGGCCGCCGCCCGGCAGGCCCAGACCATGCAGGCCCCCTCGCTCCTCAGCGTGGTGGCCGTCT GGCTGACCTGGGCCAATGGGGCCATCAACCCTGTCATCTACGCCATCCGCAATCCCAACATTTCGATGCT CCTAGGGCGCAACCGCGAGGAGGGCTACCGGACTAGGAATGTGGACGCTTTCCTGCCCAGCCAGGGCCCG GGTCTGCAAGCCAGAAGCCGCAGTCGCCTTCGAAACCGCTATGCCAACCGGCTGGGGGCCTGCAACAGGA TGTCCTCTTCCAACCCGGCCAGCGGAGTGGCAGGGGACGTGGCCATGTGGGCCCGCAAAAATCCAGTTGT ACTTTTCTGCCGAGAGGGACCACCAGAGCCGGTGACGGCAGTGACCAAACAGCCTAAATCCGAAGCTGGG GATACCAGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205463 representing NM_022571
Red=Cloning site Green=Tags(s) MEEPQPPRPPASMALLGSQHSGAPSAAGPPGGTSSAATAAVLSFSTVATAALGNLSDASGGGTAAAPGGG GLGGSGAAREAGAAVRRPLGPEAAPLLSHGAAVAAQALVLLLIFLLSSLGNCAVMGVIVKHRQLRTVTNA FILSLSLSDLLTALLCLPAAFLDLFTPPGGSAPAAAAGPWRGFCAASRFFSSCFGIVSTLSVALISLDRY CAIVRPPREKIGRRRALQLLAGAWLTALGFSLPWELLGAPRELAAAQSFHGCLYRTSPDPAQLGAAFSVG LVVACYLLPFLLMCFCHYHICKTVRLSDVRVRPVNTYARVLRFFSEVRTATTVLIMIVFVICCWGPYCFL VLLAAARQAQTMQAPSLLSVVAVWLTWANGAINPVIYAIRNPNISMLLGRNREEGYRTRNVDAFLPSQGP GLQARSRSRLRNRYANRLGACNRMSSSNPASGVAGDVAMWARKNPVVLFCREGPPEPVTAVTKQPKSEAG DTSL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022571 |
ORF Size | 1482 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022571.6 |
RefSeq Size | 1834 bp |
RefSeq ORF | 1485 bp |
Locus ID | 64582 |
UniProt ID | Q8IZ08 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | Orphan receptor. Has spontaneous activity for beta-arrestin recruitment (PubMed:28827538). Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205463 | GPR135 (Myc-DDK-tagged)-Human G protein-coupled receptor 135 (GPR135) |
CNY 3,656.00 |
|
RC205463L3 | Lenti-ORF clone of GPR135 (Myc-DDK-tagged)-Human G protein-coupled receptor 135 (GPR135) |
CNY 5,890.00 |
|
RC205463L4 | Lenti-ORF clone of GPR135 (mGFP-tagged)-Human G protein-coupled receptor 135 (GPR135) |
CNY 5,890.00 |
|
SC305036 | GPR135 (untagged)-Human G protein-coupled receptor 135 (GPR135) |
CNY 3,656.00 |