HACE1 (NM_020771) Human Tagged ORF Clone
CAT#: RG205602
- TrueORF®
HACE1 (tGFP-tagged) - Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1)
ORF Plasmid: DDK
"NM_020771" in other vectors (6)
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CNY 8,256.00
Cited in 1 publication. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SPPRS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205602 representing NM_020771
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCACGCGCGCACCGTGGAGTTGC CCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCTGATCAACACAGGTCTGTTTC TGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTGAAAAGAAGCTTGCTTCAC ATTGCAGCAAATTGTGGATCGGTGGAATGCTTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATC AAGATATTTCAGGCTGTACACCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATT ATTAGAATATAGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTG AATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAGGATGCCATGG GGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTGCAGTGCTTGCTAGACAGTGG TGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCATTGTACTTTGCTTGCAGTCATGGTCAGAGA GATACAGCACAGATCCTACTATTACGAGGAGCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGG ATTTATGTGTACAGGGTGGATATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCA GACTATTATTCAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTG TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACAACAAATGGTC ATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTTTTAAGGATTGTGAGAATGTT TTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAATGGAATTGATATGGGCTACAATGGGAATAAA ACTCCAAGAAGCCAGGTGTTCAAGCCTCTGGAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAA CAGCCACAGAATTGATGAAAAACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGG CCAAGATCAAGATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCC ACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGATTGTCAGGATG TTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTACATGTGCTGTTCTTGTCAGAT GCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTCTGCAAACATGATGAAGTTTTAAAATGCTTT GTTAATAGAAATCCCAAAATTATATTTGACCACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAA GATTCATGCATATCATAAAAGCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTC AGGACAGCCAGATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGAT TCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAAGGGATTGCTG TACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGGTTTGATATTCTGTCCAATGA GATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCTGATGGAACAACTTTTCAGCCTAATAGCAAC TCTTATGTAAATCCTGATCACTTGAACTATTTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACC ACAGGCAGCTGGTCAATATTTACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTA CCAAGATGTGGCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGT GATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAGGTGCCTTTGA AACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTACGTCCAGCTTGTTACTGAACT TCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTTTTACAGGGCTTTCATATGTTCATTCCACCC TCCCTCATACAGCTTTTTGATGAATATGAATTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGA GTGATTGGATAAAAAATACAGAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTG GGAAGTTGTAGAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGG GTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATCGCTGCTGTGC CATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTCAAGTTACCTGAATACCCAAG TAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACATTGTGGCAGCTATGGTTACACAATGGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205602 representing NM_020771
Red=Cloning site Green=Tags(s) MERAMEQLNRLTRSLRHARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLH IAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAV NGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQR DTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHL SQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNK TPRSQVFKPLELLWHSLDEWLVLTATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLS TGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCF VNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRD SIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSN SYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDIS DLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPP SLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSR VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020771 |
ORF Size | 2727 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020771.2, NP_065822.1 |
RefSeq Size | 4576 bp |
RefSeq ORF | 2730 bp |
Locus ID | 57531 |
UniProt ID | Q8IYU2 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress
,Zhang, L;Chen, X;Sharma, P;Moon, M;Sheftel, AD;Dawood, F;Nghiem, MP;Wu, J;Li, RK;Gramolini, AO;Sorensen, PH;Penninger, JM;Brumell, JH;Liu, PP;,
Nat Commun
,PubMed ID 24614889
[HACE1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205602 | HACE1 (Myc-DDK-tagged)-Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1) |
CNY 6,656.00 |
|
RC205602L1 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), Myc-DDK-tagged |
CNY 9,056.00 |
|
RC205602L2 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), mGFP tagged |
CNY 9,030.00 |
|
RC205602L3 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), Myc-DDK-tagged |
CNY 9,030.00 |
|
RC205602L4 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), mGFP tagged |
CNY 9,056.00 |
|
SC107534 | HACE1 (untagged)-Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1) |
CNY 6,664.00 |