FATE1 (NM_033085) Human Tagged ORF Clone

CAT#: RG205951

  • TrueORF®

FATE1 (tGFP-tagged) - Human fetal and adult testis expressed 1 (FATE1)

ORF Plasmid: DDK tGFP



  "NM_033085" in other vectors (4)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


FATE1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CT43; FATE
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG205951 representing NM_033085
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGAGGCCCTCCCAACACCAAGGCGGAGATGGAAATGTCCCTGGCAGAAGAACTGAATCATGGAC
GCCAAGGGGAAAACCAAGAGCACCTGGTGATAGCAGAAATGATGGAGCTTGGATCTCGGTCCCGGGGTGC
CTCCCAGAAGAAGCAGAAGTTGGAACAAAAAGCTGCTGGCTCTGCTTCAGCCAAACGAGTTTGGAATATG
ACTGCCACCCGACCCAAGAAAATGGGGTCCCAGCTGCCAAAGCCCAGAATGCTGAGAGAATCAGGCCATG
GGGATGCCCATCTCCAGGAGTACGCTGGCAATTTCCAAGGCATACGTTTCCATTATGATCGCAACCCAGG
GACAGATGCAGTGGCGCAGACTAGCCTGGAAGAGTTCAATGTACTGGAGATGGAAGTCATGAGAAGACAG
CTGTATGCAGTCAACCGGCGTCTGCGCGCCCTGGAGGAACAGGGCGCCACCTGGCGCCACAGGGAGACCC
TGATCATCGCCGTGCTGGTGTCGGCCAGCATTGCCAACCTGTGGCTGTGGATGAACCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG205951 representing NM_033085
Red=Cloning site Green=Tags(s)

MAGGPPNTKAEMEMSLAEELNHGRQGENQEHLVIAEMMELGSRSRGASQKKQKLEQKAAGSASAKRVWNM
TATRPKKMGSQLPKPRMLRESGHGDAHLQEYAGNFQGIRFHYDRNPGTDAVAQTSLEEFNVLEMEVMRRQ
LYAVNRRLRALEEQGATWRHRETLIIAVLVSASIANLWLWMNQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033085
ORF Size 549 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_033085.3
RefSeq Size 1071 bp
RefSeq ORF 552 bp
Locus ID 89885
UniProt ID Q969F0
Protein Families Transmembrane
Gene Summary This gene encodes a cancer-testis antigen that is highly expressed in hepatocellular carcinomas and other tumors and weakly expressed in normal tissues except testis. The protein is strongly expressed in spermatogonia, primary spermatocytes, and Sertoli cells in seminiferous tubules. This protein may have a role in the control of early testicular differentiation and cell proliferation. [provided by RefSeq, Jan 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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