ULK2 (NM_014683) Human Tagged ORF Clone
CAT#: RG206010
- TrueORF®
ULK2 (tGFP-tagged) - Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1
ORF Plasmid: DDK
"NM_014683" in other vectors (7)
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CNY 12,416.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ATG1B; Unc51.2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG206010 representing NM_014683
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGTGGGTGACTTCGAGTACAGCAAGAGGGATCTCGTGGGACACGGGGCCTTCGCCGTGGTCT TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTAGCTATTAAAAGTATTAATAAAAAGAACTTGTC AAAATCACAAATACTGCTTGGAAAGGAAATTAAAATCTTAAAGGAACTTCAGCATGAAAATATTGTAGCA CTCTATGATGTTCAGGAATTACCCAACTCTGTCTTTTTGGTGATGGAGTATTGCAATGGTGGAGACCTCG CAGATTATTTGCAAGCGAAAGGGACTCTCAGTGAAGACACGATCAGAGTGTTTCTGCATCAGATTGCTGC TGCCATGCGAATCCTGCACAGCAAAGGAATCATCCACAGAGATCTCAAACCACAGAACATCTTGCTGTCC TATGCCAATCGCAGAAAATCAAGTGTCAGTGGTATTCGCATCAAAATAGCGGATTTTGGTTTTGCTCGTT ACCTACATAGTAACATGATGGCTGCAACACTGTGTGGATCCCCGATGTACATGGCTCCTGAGGTTATTAT GTCTCAACATTATGATGCTAAGGCTGACTTGTGGAGCATAGGAACAGTGATATACCAATGCCTAGTTGGA AAACCACCTTTTCAGGCCAATAGTCCTCAAGACTTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC CTAGTATTCCCAGAGAAACATCACCTTATTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAACCAAAA AGATAGAATGGACTTTGAAGCGTTTTTTAGCCATCCTTTTCTTGAGCAAGGTCCAGTAAAAAAATCTTGC CCAGTTCCAGTGCCCATGTATTCTGGTTCTGTCTCTGGAAGCTCCTGTGGCAGCTCTCCATCTTGTCGTT TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCTTCCCCACCATTGGG TCCTCCCAACTATCTACAAGTTTCCAAAGATTCTGCCAGTACTAGTAGCAAGAACTCTTCTTGTGACACG GATGACTTTGTTTTGGTGCCACACAACATCTCGTCAGACCACTCATGTGATATGCCAATGGGGACTGCTG GCAGACGTGCTTCAAATGAATTCTTGGTGTGTGGAGGGCAGTGTCAGCCTACTGTGTCACCTCACAGCGA AACAGCACCAATTCCAGTTCCTACTCAAATAAGGAATTATCAGCGCATAGAGCAGAATCTTACATCTACT GCCAGCTCAGGCACAAATGTACATGGTTCTCCAAGATCTGCAGTGGTACGAAGGTCCAACACCAGCCCCA TGGGCTTCCTCCGGCCGGGATCATGCTCCCCAGTACCAGCAGACACAGCACAGACAGTTGGACGAAGGCT CTCCACTGGGTCTTCTAGGCCTTACTCACCTTCCCCTTTGGTTGGTACCATTCCTGAGCAATTCAGTCAG TGCTGCTGTGGGCATCCTCAGGGCCATGACTCCAGGAGTAGAAACTCCTCAGGTTCTCCAGTGCCACAAG CTCAGTCCCCACAGTCTCTCTTATCGGGTGCTAGACTGCAGAGCGCCCCCACCCTCACTGACATCTATCA GAACAAGCAGAAGCTCAGAAAACAGCACTCTGACCCCGTGTGCCCATCCCATACTGGGGCTGGGTACAGC TACTCGCCTCAGCCCAGTCGGCCTGGCAGCCTTGGAACTTCTCCCACCAAGCACTTGGGGTCCTCTCCAC GGAGTTCTGACTGGTTCTTTAAAACTCCTTTGCCAACAATCATTGGCTCTCCTACTAAGACCACAGCTCC TTTCAAAATCCCTAAAACTCAAGCATCTTCCAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA GAACAGTCGAAAGATGGGAATGAGCCACGGGAATGTGCCCATTGCCTCTTAGTGCAAGGAAGTGAGAGGC AGCGGGCCGAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACCGGGAAGTTATCAGATCAACA AGGAAAGACTCCTATATGTCGACATCAGGGCAGCACAGACAGTTTAAATACAGAACGACCAATGGATATA GCTCCGGCAGGAGCCTGTGGTGGTGTTCTGGCACCTCCTGCAGGTACAGCAGCAAGTTCCAAGGCTGTCC TCTTCACTGTAGGGTCTCCTCCACACAGTGCGGCAGCCCCCACTTGTACCCACATGTTCCTTCGAACAAG AACAACCTCAGTGGGGCCCAGCAACTCCGGGGGCTCTCTTTGTGCCATGAGTGGCCGCGTGTGCGTGGGG TCCCCGCCTGGCCCAGGCTTCGGCTCTTCCCCTCCAGGAGCAGAGGCAGCTCCCAGCCTGAGATACGTGC CTTACGGTGCTTCACCCCCCAGCCTAGAGGGGCTCATCACCTTTGAAGCCCCTGAACTGCCGGAGGAGAC GCTGATGGAGCGGGAACACACAGACACCTTACGCCATCTGAATGTGATGCTGATGTTCACTGAGTGTGTG CTGGACCTGACAGCCATGAGGGGAGGAAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGATCC AGGAGAGTGTGGTGGTGGACCAGATCAGTCAGCTGAGCAAAGACTGGGGGCGGGTGGAGCAGCTGGTGTT GTACATGAAAGCAGCACAGCTGCTTGCGGCTTCTCTGCATCTTGCCAAAGCCCAGATCAAGTCCGGGAAA CTGAGCCCATCCACAGCTGTGAAACAAGTTGTCAAGAATCTGAACGAACGATATAAATTCTGCATCACCA TGTGCAAGAAACTTACAGAAAAGCTGAATCGATTCTTCTCTGACAAACAGAGGTTTATTGATGAAATCAA CAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTAGAAATGGTTCAGTCTGCAGCCCTGGATGAG ATGTTTCAGCAGACCGAAGATATTGTTTATCGCTATCATAAGGCAGCCCTTCTTTTGGAAGGCCTAAGTA GGATTCTACAGGACCCTGCAGATATTGAAAATGTGCATAAATATAAATGTAGTATTGAGAGAAGACTGTC GGCGCTCTGCCATAGCACCGCAACCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206010 representing NM_014683
Red=Cloning site Green=Tags(s) MEVVGDFEYSKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVA LYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGIIHRDLKPQNILLS YANRRKSSVSGIRIKIADFGFARYLHSNMMAATLCGSPMYMAPEVIMSQHYDAKADLWSIGTVIYQCLVG KPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLLLGLLQRNQKDRMDFEAFFSHPFLEQGPVKKSC PVPVPMYSGSVSGSSCGSSPSCRFASPPSLPDMQHIQEENLSSPPLGPPNYLQVSKDSASTSSKNSSCDT DDFVLVPHNISSDHSCDMPMGTAGRRASNEFLVCGGQCQPTVSPHSETAPIPVPTQIRNYQRIEQNLTST ASSGTNVHGSPRSAVVRRSNTSPMGFLRPGSCSPVPADTAQTVGRRLSTGSSRPYSPSPLVGTIPEQFSQ CCCGHPQGHDSRSRNSSGSPVPQAQSPQSLLSGARLQSAPTLTDIYQNKQKLRKQHSDPVCPSHTGAGYS YSPQPSRPGSLGTSPTKHLGSSPRSSDWFFKTPLPTIIGSPTKTTAPFKIPKTQASSNLLALVTRHGPAE EQSKDGNEPRECAHCLLVQGSERQRAEQQSKAVFGRSVSTGKLSDQQGKTPICRHQGSTDSLNTERPMDI APAGACGGVLAPPAGTAASSKAVLFTVGSPPHSAAAPTCTHMFLRTRTTSVGPSNSGGSLCAMSGRVCVG SPPGPGFGSSPPGAEAAPSLRYVPYGASPPSLEGLITFEAPELPEETLMEREHTDTLRHLNVMLMFTECV LDLTAMRGGNPELCTSAVSLYQIQESVVVDQISQLSKDWGRVEQLVLYMKAAQLLAASLHLAKAQIKSGK LSPSTAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAVEMVQSAALDE MFQQTEDIVYRYHKAALLLEGLSRILQDPADIENVHKYKCSIERRLSALCHSTATV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014683 |
ORF Size | 3108 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014683.2, NP_055498.2 |
RefSeq Size | 5807 bp |
RefSeq ORF | 3111 bp |
Locus ID | 9706 |
UniProt ID | Q8IYT8 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | mTOR signaling pathway, Regulation of autophagy |
Gene Summary | This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206010 | ULK2 (Myc-DDK-tagged)-Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1 |
CNY 10,816.00 |
|
RC206010L1 | Lenti ORF clone of Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1, Myc-DDK-tagged |
CNY 13,216.00 |
|
RC206010L2 | Lenti ORF clone of Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1, mGFP tagged |
CNY 13,216.00 |
|
RC206010L3 | Lenti ORF clone of Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1, Myc-DDK-tagged |
CNY 13,216.00 |
|
RC206010L4 | Lenti ORF clone of Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1, mGFP tagged |
CNY 13,216.00 |
|
SC114908 | ULK2 (untagged)-Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1 |
CNY 7,224.00 |
|
SC323381 | ULK2 (untagged)-Kinase deficient mutant (K39M) of Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1 |
CNY 7,224.00 |