RILP (NM_031430) Human Tagged ORF Clone

CAT#: RG206400

  • TrueORF®

RILP (tGFP-tagged) - Human Rab interacting lysosomal protein (RILP)

ORF Plasmid: DDK tGFP



  "NM_031430" in other vectors (6)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal RILP Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms PP10141
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG206400 representing NM_031430
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCAGGAGGGCGGCGCCCGGGGTGCCTGGCTGGGGGTCTCGGGAGGCCGCGGGGTCGGCATCGG
CCGCGGAGCTTGTGTACCATCTAGCCGGGGCCCTGGGCACTGAGCTGCAGGATCTGGCGCGCCGTTTCGG
GCCGGAGGCGGCGGCCGGGCTGGTGCCGCTAGTGGTGCGGGCGCTGGAGCTCTTGGAACAGGCTGCCGTG
GGGCCCGCCCCGGACTCGCTGCAGGTGTCGGCGCAGCCGGCGGAGCAGGAGCTGCGGCGGCTGCGGGAGG
AGAACGAGCGCCTCCGCAGGGAGCTGCGCGCGGGGCCACAGGAGGAGCGCGCGCTGCTGCGGCAGCTCAA
GGAGGTCACGGACCGACAGCGGGACGAACTCCGGGCGCACAACCGCGACCTGCGGCAGCGCGGCCAGGAG
ACCGAGGCGTTGCAGGAGCAGCTGCAGCGCCTCCTGCTGGTGAACGCTGAGCTGCGGCACAAGCTGGCGG
CCATGCAGACCCAGCTGCGCGCCGCGCAGGACCGCGAGAGGGAGCGCCAGCAGCCTGGCGAAGCCGCGAC
TCCGCAGGCTAAAGAGCGAGCGCGGGGGCAGGCCGGGCGGCCCGGGCACCAGCACGGACAGGAGCCCGAA
TGGGCGACCGCCGGCGCAGGCGCCCCAGGGAACCCTGAGGACCCGGCGGAGGCCGCGCAGCAGCTCGGGC
GCCCCTCGGAGGCAGGGCAGTGCCGCTTCAGTCGGGAGGAGTTTGAGCAGATCCTTCAGGAGCGGAATGA
ACTCAAAGCCAAAGTGTTCCTGCTCAAGGAGGAACTGGCCTACTTCCAGCGGGAGCTGCTCACAGACCAC
CGGGTCCCCAGCCTTCTGCTCGAGGCCATGAAGGTGGCTGTCCGGAAGCAGCGGAAGAAGATCAAGGCCA
AGATGTTAGGGACACCAGAGGAAGCAGAGAGCAGTGAGGATGAGGCTGGCCCATGGATCCTGCTCTCCGA
TGACAAGGGAGACCATCCCCCACCCCCGGAGTCCAAAATACAGAGTTTCTTTGGCCTATGGTATCGGGGT
AAAGCTGAATCCTCTGAGGATGAGACCAGCAGCCCTGCACCCAGCAAGCTAGGGGGAGAAGAGGAGGCCC
AACCACAGTCTCCAGCTCCTGATCCGCCCTGTTCTGCCCTCCACGAACACCTTTGTCTGGGGGCCTCAGC
CGCCCCAGAGGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG206400 representing NM_031430
Red=Cloning site Green=Tags(s)

MEPRRAAPGVPGWGSREAAGSASAAELVYHLAGALGTELQDLARRFGPEAAAGLVPLVVRALELLEQAAV
GPAPDSLQVSAQPAEQELRRLREENERLRRELRAGPQEERALLRQLKEVTDRQRDELRAHNRDLRQRGQE
TEALQEQLQRLLLVNAELRHKLAAMQTQLRAAQDRERERQQPGEAATPQAKERARGQAGRPGHQHGQEPE
WATAGAGAPGNPEDPAEAAQQLGRPSEAGQCRFSREEFEQILQERNELKAKVFLLKEELAYFQRELLTDH
RVPSLLLEAMKVAVRKQRKKIKAKMLGTPEEAESSEDEAGPWILLSDDKGDHPPPPESKIQSFFGLWYRG
KAESSEDETSSPAPSKLGGEEEAQPQSPAPDPPCSALHEHLCLGASAAPEA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031430
ORF Size 1203 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031430.1, NP_113618.1
RefSeq Size 1811 bp
RefSeq ORF 1206 bp
Locus ID 83547
UniProt ID Q96NA2
Gene Summary This gene encodes a lysosomal protein that interacts with RAB7, a small GTPase that controls transport to endocytic degradative compartments. Studies using mutant forms of the two proteins suggest that this protein represents a downstream effector for RAB7, and both proteins act together in the regulation of late endocytic traffic. A unique region of this protein has also been shown to be involved in the regulation of lysosomal morphology. [provided by RefSeq, Sep 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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