United States
Germany
Japan
United Kingdom
China
PKC beta 1 (PRKCB) (NM_002738) Human Tagged ORF Clone
CAT＃: RG207217
- TrueORF®
PRKCB (tGFP-tagged) - Human protein kinase C, beta (PRKCB), transcript variant 2
ORF Plasmid: DDK
"NM_002738" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 8,992.00
| Cited in 1 publication. |
Product images
CNY 1,000.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1; PRKCB2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207217 representing NM_002738
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACCCGGCTGCGGGGCCGCCGCCGAGCGAGGGCGAGGAGAGCACCGTGCGCTTCGCCCGCAAAG GCGCCCTCCGGCAGAAGAACGTGCATGAGGTCAAGAACCACAAATTCACCGCCCGCTTCTTCAAGCAGCC CACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGCTTCGGGAAGCAGGGATTCCAGTGCCAAGTTTGC TGCTTTGTGGTGCACAAGCGGTGCCATGAATTTGTCACATTCTCCTGCCCTGGCGCTGACAAGGGTCCAG CCTCCGATGACCCCCGCAGCAAACACAAGTTTAAGATCCACACGTACTCCAGCCCCACGTTTTGTGACCA CTGTGGGTCACTGCTGTATGGACTCATCCACCAGGGGATGAAATGTGACACCTGCATGATGAATGTGCAC AAGCGCTGCGTGATGAATGTTCCCAGCCTGTGTGGCACGGACCACACGGAGCGCCGCGGCCGCATCTACA TCCAGGCCCACATCGACAGGGACGTCCTCATTGTCCTCGTAAGAGATGCTAAAAACCTTGTACCTATGGA CCCCAATGGCCTGTCAGATCCCTACGTAAAACTGAAACTGATTCCCGATCCCAAAAGTGAGAGCAAACAG AAGACCAAAACCATCAAATGCTCCCTCAACCCTGAGTGGAATGAGACATTTAGATTTCAGCTGAAAGAAT CGGACAAAGACAGAAGACTGTCAGTAGAGATTTGGGATTGGGATTTGACCAGCAGGAATGACTTCATGGG ATCTTTGTCCTTTGGGATTTCTGAACTTCAGAAAGCCAGTGTTGATGGCTGGTTTAAGTTACTGAGCCAG GAGGAAGGCGAGTACTTCAATGTGCCTGTGCCACCAGAAGGAAGTGAGGCCAATGAAGAACTGCGGCAGA AATTTGAGAGGGCCAAGATCAGTCAGGGAACCAAGGTCCCGGAAGAAAAGACGACCAACACTGTCTCCAA ATTTGACAACAATGGCAACAGAGACCGGATGAAACTGACCGATTTTAACTTCCTAATGGTGCTGGGGAAA GGCAGCTTTGGCAAGGTCATGCTTTCAGAACGAAAAGGCACAGATGAGCTCTATGCTGTGAAGATCCTGA AGAAGGACGTTGTGATCCAAGATGATGACGTGGAGTGCACTATGGTGGAGAAGCGGGTGTTGGCCCTGCC TGGGAAGCCGCCCTTCCTGACCCAGCTCCACTCCTGCTTCCAGACCATGGACCGCCTGTACTTTGTGATG GAGTACGTGAATGGGGGCGACCTCATGTATCACATCCAGCAAGTCGGCCGGTTCAAGGAGCCCCATGCTG TATTTTACGCTGCAGAAATTGCCATCGGTCTGTTCTTCTTACAGAGTAAGGGCATCATTTACCGTGACCT AAAACTTGACAACGTGATGCTCGATTCTGAGGGACACATCAAGATTGCCGATTTTGGCATGTGTAAGGAA AACATCTGGGATGGGGTGACAACCAAGACATTCTGTGGCACTCCAGACTACATCGCCCCCGAGATAATTG CTTATCAGCCCTATGGGAAGTCCGTGGATTGGTGGGCATTTGGAGTCCTGCTGTATGAAATGTTGGCTGG GCAGGCACCCTTTGAAGGGGAGGATGAAGATGAACTCTTCCAATCCATCATGGAACACAACGTAGCCTAT CCCAAGTCTATGTCCAAGGAAGCTGTGGCCATCTGCAAAGGGCTGATGACCAAACACCCAGGCAAACGTC TGGGTTGTGGACCTGAAGGCGAACGTGATATCAAAGAGCATGCATTTTTCCGGTATATTGATTGGGAGAA ACTTGAACGCAAAGAGATCCAGCCCCCTTATAAGCCAAAAGCTTGTGGGCGAAATGCTGAAAACTTCGAC CGATTTTTCACCCGCCATCCACCAGTCCTAACACCTCCCGACCAGGAAGTCATCAGGAATATTGACCAAT CAGAATTCGAAGGATTTTCCTTTGTTAACTCTGAATTTTTAAAACCCGAAGTCAAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207217 representing NM_002738
Red=Cloning site Green=Tags(s) MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVC CFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVH KRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQ KTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQ EEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGK GSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVM EYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKE NIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAY PKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFD RFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002738 |
| ORF Size | 2019 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002738.7 |
| RefSeq Size | 3411 bp |
| RefSeq ORF | 2022 bp |
| Locus ID | 5579 |
| UniProt ID | P05771 |
| Domains | C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | B cell receptor signaling pathway, Calcium signaling pathway, Chemokine signaling pathway, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway |
| Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cross-Desensitization of CCR1, but Not CCR2, following Activation of the Formyl Peptide Receptor FPR1
,Bednar, F;Song, C;Bardi, G;Cornwell, W;Rogers, TJ;,
J. Immunol.
,PubMed ID 24778447
[PRKCB]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207217 | PRKCB (Myc-DDK-tagged)-Human protein kinase C, beta (PRKCB), transcript variant 2 |
CNY 7,392.00 |
|
| RC207217L1 | Lenti ORF clone of Human protein kinase C, beta (PRKCB), transcript variant 2, Myc-DDK-tagged |
CNY 9,792.00 |
|
| RC207217L2 | Lenti ORF clone of Human protein kinase C, beta (PRKCB), transcript variant 2, mGFP tagged |
CNY 9,792.00 |
|
| RC207217L3 | Lenti ORF clone of Human protein kinase C, beta (PRKCB), transcript variant 2, Myc-DDK-tagged |
CNY 9,792.00 |
|
| RC207217L4 | Lenti ORF clone of Human protein kinase C, beta (PRKCB), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC118454 | PRKCB (untagged)-Human protein kinase C, beta (PRKCB), transcript variant 2 |
CNY 7,408.00 |
|
| SC323585 | PRKCB (untagged)-Kinase deficient mutant (K371M) of Human protein kinase C, beta (PRKCB), transcript variant 2 |
CNY 7,408.00 |
其它PKC beta 1产品
