P2RX4 (NM_002560) Human Tagged ORF Clone
CAT#: RG207613
- TrueORF®
P2RX4 (tGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4)
ORF Plasmid: DDK
"NM_002560" in other vectors (7)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | P2X4; P2X4R |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG207613 representing NM_002560
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCTGCTGCGCCGCGCTGGCGGCCTTCCTGTTCGAGTACGACACGCCGCGCATCGTGCTCATCC GCAGCCGCAAAGTGGGGCTCATGAACCGCGCCGTGCAACTGCTCATCCTGGCCTACGTCATCGGGTGGGT GTTTGTGTGGGAAAAGGGCTACCAGGAAACTGACTCCGTGGTCAGCTCCGTTACGACCAAGGTCAAGGGC GTGGCTGTGACCAACACTTCTAAACTTGGATTCCGGATCTGGGATGTGGCGGATTATGTGATACCAGCTC AGGAGGAAAACTCCCTCTTCGTCATGACCAACGTGATCCTCACCATGAACCAGACACAGGGCCTGTGCCC CGAGATTCCAGATGCGACCACTGTGTGTAAATCAGATGCCAGCTGTACTGCCGGCTCTGCCGGCACCCAC AGCAACGGAGTCTCAACAGGCAGGTGCGTAGCTTTCAACGGGTCCGTCAAGACGTGTGAGGTGGCGGCCT GGTGCCCGGTGGAGGATGACACACACGTGCCACAACCTGCTTTTTTAAAGGCTGCAGAAAACTTCACTCT TTTGGTTAAGAACAACATCTGGTATCCCAAATTTAATTTCAGCAAGAGGAATATCCTTCCCAACATCACC ACTACTTACCTCAAGTCGTGCATTTATGATGCTAAAACAGATCCCTTCTGCCCCATATTCCGTCTTGGCA AAATAGTGGAGAACGCAGGACACGGTTTCCAGGACATGGCCGTGGAGGGAGGCATCATGGGCATCCAGGT CAACTGGGACTGCAACCTGGACAGAGCCGCCTCCCTCTGCTTGCCCAGGTACTCCTTCCGCCGCCTCGAT ACACGGGACGTTGAGCACAACGTATCTCCTGGCTACAATTTCAGGTTTGCCAAGTACTACAGAGACCTGG CTGGCAACGAGCAGCGCACGCTCATCAAGGCCTATGGCATCCGCTTCGACATCATTGTGTTTGGGAAGGC AGGGAAATTTGACATCATCCCCACTATGATCAACATCGGCTCTGGCCTGGCACTGCTAGGCATGGCGACC GTGCTGTGTGACATCATAGTCCTCTACTGCATGAAGAAAAGACTCTACTATCGGGAGAAGAAATATAAAT ATGTGGAAGATTACGAGCAGGGTCTTGCTAGTGAGCTGGACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207613 representing NM_002560
Red=Cloning site Green=Tags(s) MAGCCAALAAFLFEYDTPRIVLIRSRKVGLMNRAVQLLILAYVIGWVFVWEKGYQETDSVVSSVTTKVKG VAVTNTSKLGFRIWDVADYVIPAQEENSLFVMTNVILTMNQTQGLCPEIPDATTVCKSDASCTAGSAGTH SNGVSTGRCVAFNGSVKTCEVAAWCPVEDDTHVPQPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNIT TTYLKSCIYDAKTDPFCPIFRLGKIVENAGHGFQDMAVEGGIMGIQVNWDCNLDRAASLCLPRYSFRRLD TRDVEHNVSPGYNFRFAKYYRDLAGNEQRTLIKAYGIRFDIIVFGKAGKFDIIPTMINIGSGLALLGMAT VLCDIIVLYCMKKRLYYREKKYKYVEDYEQGLASELDQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002560 |
ORF Size | 1164 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002560.2, NP_002551.2 |
RefSeq Size | 2043 bp |
RefSeq ORF | 1167 bp |
Locus ID | 5025 |
UniProt ID | Q99571 |
Domains | P2X_receptor |
Protein Families | Druggable Genome, Ion Channels: ATP Receptors, Transmembrane |
Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
Gene Summary | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207613 | P2RX4 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4) |
CNY 3,656.00 |
|
RC207613L1 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC207613L2 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), mGFP tagged |
CNY 5,890.00 |
|
RC207613L3 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC207613L4 | Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), mGFP tagged |
CNY 6,056.00 |
|
SC122124 | P2RX4 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4) |
CNY 3,656.00 |
|
SC324305 | P2RX4 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4) |
CNY 3,656.00 |