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HIRA (NM_003325) Human Tagged ORF Clone
CAT＃: RG208049
- TrueORF®
HIRA (tGFP-tagged) - Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA)
ORF Plasmid: DDK
"NM_003325" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DGCR1; TUP1; TUPLE1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208049 representing NM_003325
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTCCTGAAGCCGACCTGGGTCAACCACAATGGCAAGCCGATTTTTTCAGTTGATATTCACCCTG ACGGGACCAAGTTCGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTTGTGATCTGGAATATGTCTCC AGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCCAAGATGCTTTGCCAGATGGACAATCACTTA GCATGTGTGAACTGTGTGCGGTGGTCAAACAGTGGGATGTATTTAGCTTCTGGGGGAGATGACAAACTGA TTATGGTGTGGAAGCGGGCTACGTACATCGGCCCCAGCACCGTGTTCGGCTCCAGTGGTAAGCTTGCCAA TGTGGAGCAGTGGCGGTGTGTCTCTATCCTCCGGAATCATTCAGGCGATGTGATGGATGTAGCATGGTCT CCCCACGATGCCTGGCTAGCCTCATGCAGCGTGGATAACACTGTCGTCATCTGGAATGCTGTAAAGTTCC CAGAAATTCTAGCTACTCTGAGAGGTCATTCTGGCTTGGTCAAAGGGTTGACATGGGACCCTGTTGGTAA ATACATAGCTTCTCAAGCTGATGACCGCAGCCTAAAGGTGTGGAGGACGCTGGACTGGCAGTTGGAGACC AGCATCACCAAGCCTTTTGATGAGTGTGGAGGAACGACCCATGTGTTGCGGCTCAGCTGGTCACCTGATG GGCATTACCTGGTGTCTGCCCATGCCATGAACAACTCAGGCCCCACTGCCCAGATCATCGAACGGGAGGG ATGGAAGACCAACATGGACTTTGTTGGGCACCGGAAAGCTGTGACTGTCGTGAAATTCAACCCAAAAATC TTCAAAAAGAAGCAGAAGAATGGGAGTTCTGCGAAGCCTAGCTGCCCGTACTGCTGCTGTGCTGTTGGCA GCAAGGACCGCTCGCTTTCTGTCTGGCTCACATGTCTGAAACGGCCGCTGGTGGTCATCCATGAACTGTT TGACAAATCCATCATGGATATTTCCTGGACTCTGAATGGGCTGGGCATCTTGGTATGCTCTATGGACGGC TCTGTGGCATTCCTCGACTTCTCCCAGGATGAGCTTGGCGATCCCCTGAGCGAGGAGGAGAAGAGCCGCA TTCACCAGTCCACCTATGGCAAGAGCCTAGCCATCATGACCGAGGCCCAGCTCTCCACAGCCGTCATTGA GAACCCTGAGATGCTCAAGTACCAGCGAAGGCAGCAGCAGCAGCAGCTGGACCAGAAGAGTGCTGCGACC AGGGAGATGGGCTCAGCCACCTCAGTCGCAGGCGTTGTCAACGGGGAGAGTCTTGAAGATATCAGGAAGA ATCTTTTGAAGAAACAAGTTGAGACTCGGACAGCAGATGGCCGGAGAAGAATCACGCCTCTCTGCATAGC ACAGCTGGACACTGGGGACTTCTCCACGGCATTCTTTAACAGCATCCCCCTCTCGGGCTCCCTGGCGGGC ACCATGCTCTCTTCTCATAGCAGTCCACAGCTACTGCCACTGGACTCCAGTACCCCTAACTCCTTCGGCG CCTCGAAGCCTTGCACAGAGCCTGTGGTGGCTGCCAGTGCCAGACCTGCAGGCGATTCTGTCAATAAAGA CAGTATGAATGCTACCTCTACTCCTGCTGCATTGTCACCTTCTGTGTTAACGACCCCGTCCAAGATCGAA CCCATGAAAGCGTTTGACTCCCGGTTCACAGAGCGGTCCAAAGCCACACCAGGTGCTCCTGCCCTGACCA GCATGACTCCGACAGCTGTGGAAAGGTTAAAAGAGCAGAACCTTGTGAAAGAGCTGAGGCCCCGAGACCT CCTGGAGAGCAGCAGTGACAGCGATGAGAAAGTCCCTTTGGCTAAGGCTTCCTCACTGTCCAAGCGAAAA CTTGAGCTTGAGGTAGAGACAGTAGAGAAGAAGAAGAAAGGGCGGCCTCGGAAGGACTCTCGTCTCATGC CTGTGTCTCTGTCTGTCCAGTCTCCAGCTGCCCTAACCGCAGAGAAGGAGGCCATGTGTCTGTCTGCACC AGCACTTGCACTGAAGCTGCCAATTCCAAGCCCCCAGAGAGCATTCACCCTCCAGGTCAGCTCCGATCCT TCCATGTACATTGAGGTGGAGAATGAAGTGACAGTGGTGGGGGGCGTGAAGCTGAGCCGCCTGAAGTGCA ACCGGGAAGGGAAGGAGTGGGAGACGGTACTCACCAGCCGGATCCTCACTGCTGCGGGCAGCTGTGACGT GGTGTGTGTCGCCTGTGAAAAAAGGATGCTGTCAGTGTTCTCCACCTGTGGTCGCCGTCTCCTCTCTCCC ATCCTCCTGCCATCCCCGATCTCTACTTTGCATTGCACAGGCTCCTACGTCATGGCGCTCACCGCTGCAG CCACACTCTCTGTCTGGGATGTTCACAGACAGGTGGTTGTGGTGAAAGAAGAGTCTCTACACTCCATCCT GGCAGGAAGTGATATGACGGTATCACAGATCTTGCTGACGCAGCATGGAATCCCAGTAATGAACCTGTCC GATGGGAAGGCGTACTGCTTTAATCCGTCACTTTCCACATGGAACCTGGTTTCTGACAAGCAGGACTCAC TGGCTCAGTGTGCAGACTTTAGGAGCAGCCTGCCATCCCAGGACGCCATGCTGTGCTCAGGACCGTTAGC CATAATCCAGGGCCGCACCTCCAACTCGGGAAGGCAGGCTGCCCGGCTCTTCTCCGTGCCTCATGTGGTG CAGCAAGAGACCACCCTGGCCTACCTAGAGAACCAGGTGGCAGCAGCACTCACCCTGCAGTCCAGCCACG AGTACCGCCATTGGCTCCTCGTCTACGCACGGTACCTCGTAAACGAAGGGTTTGAATACCGACTTCGAGA AATATGCAAGGACTTACTGGGTCCGGTTCACTACTCCACTGGAAGCCAGTGGGAGTCAACAGTAGTGGGT CTGCGGAAGAGGGAGCTGCTGAAGGAGCTGCTACCAGTCATCGGGCAGAACCTCCGATTCCAGCGCCTCT TCACCGAGTGTCAGGAACAGCTCGACATCCTGAGGGACAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208049 representing NM_003325
Red=Cloning site Green=Tags(s) MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHL ACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWS PHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLET SITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKI FKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDG SVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAAT REMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAG TMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIE PMKAFDSRFTERSKATPGAPALTSMTPTAVERLKEQNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRK LELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAALTAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDP SMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSP ILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQVVVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLS DGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVV QQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVG LRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003325 |
| ORF Size | 3051 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003325.4 |
| RefSeq Size | 4013 bp |
| RefSeq ORF | 3054 bp |
| Locus ID | 7290 |
| UniProt ID | P54198 |
| Domains | WD40 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208049 | HIRA (Myc-DDK-tagged)-Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA) |
CNY 7,080.00 |
|
| RC208049L3 | Lenti-ORF clone of HIRA (Myc-DDK-tagged)-Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA) |
CNY 10,260.00 |
|
| RC208049L4 | Lenti-ORF clone of HIRA (mGFP-tagged)-Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA) |
CNY 10,260.00 |
|
| SC311322 | HIRA (untagged)-Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA) |
CNY 10,450.00 |
