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OLIG2 (NM_005806) Human Tagged ORF Clone
CAT＃: RG208209
- TrueORF®
OLIG2 (tGFP-tagged) - Human oligodendrocyte lineage transcription factor 2 (OLIG2)
ORF Plasmid: DDK
"NM_005806" in other vectors (7)
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CNY 4,000.00
CNY 4,370.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BHLHB1; bHLHe19; OLIGO2; PRKCBP2; RACK17 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208209 representing NM_005806
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGACGCCAGCCTGGTGTCCAGCCGCCCGTCGTCGCCAGAGCCCGATGACCTTTTTCTGCCGG CCCGGAGTAAGGGCAGCAGCGGCAGCGCCTTCACTGGGGGCACCGTGTCCTCGTCCACCCCGAGTGACTG CCCGCCGGAGCTGAGCGCCGAGCTGCGCGGCGCTATGGGCTCTGCGGGCGCGCATCCTGGGGACAAGCTA GGAGGCAGTGGCTTCAAGTCATCCTCGTCCAGCACCTCGTCGTCTACGTCGTCGGCGGCTGCGTCGTCCA CCAAGAAGGACAAGAAGCAAATGACAGAGCCGGAGCTGCAGCAGCTGCGTCTCAAGATCAACAGCCGCGA GCGCAAGCGCATGCACGACCTCAACATCGCCATGGATGGCCTCCGCGAGGTCATGCCGTACGCACACGGC CCTTCGGTGCGCAAGCTTTCCAAGATCACCACGCTGCTGCTGGCGCGCAACTACATCCTCATGCTCACCA ACTCGCTGGAGGAGATGAAGCGACTGGTGAGCGAGATCTACGGGGGCCACCACGCTGGCTTCCACCCGTC GGCCTGCGGCGGCCTGGCGCACTCCGCGCCCCTGCCCGCCGCCACCGCGCACCCGGCAGCAGCAGCGCAC GCCGCACATCACCCCGCGGTGCACCACCCCATCCTGCCGCCCGCCGCCGCAGCGGCTGCTGCCGCCGCTG CAGCCGCGGCTGTGTCCAGCGCCTCTCTGCCCGGATCCGGGCTGCCGTCGGTCGGCTCAATCCGTCCACC GCACGGCCTACTCAAGTCTCCGTCTGCTGCCGCGGCCGCCCCGCTGGGGGGCGGGGGCGGCGGCAGTGGG GCGAGCGGGGGCTTCCAGCACTGGGGCGGCATGCCCTGCCCCTGCAGCATGTGCCAGGTGCCGCCGCCGC ACCACCACGTGTCGGCTATGGGCGCCGGCAGCCTGCCGCGCCTCACCTCCGACGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208209 representing NM_005806
Red=Cloning site Green=Tags(s) MDSDASLVSSRPSSPEPDDLFLPARSKGSSGSAFTGGTVSSSTPSDCPPELSAELRGAMGSAGAHPGDKL GGSGFKSSSSSTSSSTSSAAASSTKKDKKQMTEPELQQLRLKINSRERKRMHDLNIAMDGLREVMPYAHG PSVRKLSKITTLLLARNYILMLTNSLEEMKRLVSEIYGGHHAGFHPSACGGLAHSAPLPAATAHPAAAAH AAHHPAVHHPILPPAAAAAAAAAAAAAVSSASLPGSGLPSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSG ASGGFQHWGGMPCPCSMCQVPPPHHHVSAMGAGSLPRLTSDAK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005806 |
| ORF Size | 969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005806.2, NP_005797.1 |
| RefSeq Size | 2505 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 10215 |
| UniProt ID | Q13516 |
| Domains | HLH |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Enhancing Oligodendrocyte Differentiation by Transient Transcription Activation via DNA Nanoparticle-Mediated Transfection
,Li, X;Tzeng, SY;Zamboni, CG;Koliatsos, VE;Ming, GL;Green, JJ;Mao, HQ;,
Acta Biomater
,PubMed ID 28344151
[OLIG2]
|
|
Nanoparticle-mediated conversion of primary human astrocytes into neurons and oligodendrocytes
,Li, X;Kozielski, K;Cheng, YH;Liu, H;Zamboni, CG;,
Biomater. Sci.
,PubMed ID 27328202
[OLIG2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208209 | OLIG2 (Myc-DDK-tagged)-Human oligodendrocyte lineage transcription factor 2 (OLIG2) |
CNY 2,400.00 |
|
| RC208209L1 | Lenti ORF clone of Human oligodendrocyte lineage transcription factor 2 (OLIG2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208209L2 | Lenti ORF clone of Human oligodendrocyte lineage transcription factor 2 (OLIG2), mGFP tagged |
CNY 4,800.00 |
|
| RC208209L3 | Lenti ORF clone of Human oligodendrocyte lineage transcription factor 2 (OLIG2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208209L4 | Lenti ORF clone of Human oligodendrocyte lineage transcription factor 2 (OLIG2), mGFP tagged |
CNY 5,890.00 |
|
| SC103224 | OLIG2 (untagged)-Human oligodendrocyte lineage transcription factor 2 (OLIG2) |
CNY 2,400.00 |
|
| SC322126 | OLIG2 (untagged)-Human oligodendrocyte lineage transcription factor 2 (OLIG2) |
CNY 2,400.00 |
