GPC1 (NM_002081) Human Tagged ORF Clone
CAT#: RG208602
- TrueORF®
GPC1 (tGFP-tagged) - Human glypican 1 (GPC1)
ORF Plasmid: DDK
"NM_002081" in other vectors (4)
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CNY 5,752.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | glypican |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208602 representing NM_002081
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCGGGCCCGAGGCTGGTGGCTGCTATGTGCGGCCGCAGCGCTGGTCGCCTGCGCCCGCGGGG ACCCGGCCAGCAAGAGCCGGAGCTGCGGCGAGGTCCGCCAGATCTACGGAGCCAAGGGCTTCAGCCTGAG CGACGTGCCCCAGGCGGAGATCTCGGGTGAGCACCTGCGGATCTGTCCCCAGGGCTACACCTGCTGCACC AGCGAGATGGAGGAGAACCTGGCCAACCGCAGCCATGCCGAGCTGGAGACCGCGCTCCGGGACAGCAGCC GCGTCCTGCAGGCCATGCTTGCCACCCAGCTGCGCAGCTTCGATGACCACTTCCAGCACCTGCTGAACGA CTCGGAGCGGACGCTGCAGGCCACCTTCCCCGGCGCCTTCGGAGAGCTGTACACGCAGAACGCGAGGGCC TTCCGGGACCTGTACTCAGAGCTGCGCCTGTACTACCGCGGTGCCAACCTGCACCTGGAGGAGACGCTGG CCGAGTTCTGGGCCCGCCTGCTCGAGCGCCTCTTCAAGCAGCTGCACCCCCAGCTGCTGCTGCCTGATGA CTACCTGGACTGCCTGGGCAAGCAGGCCGAGGCGCTGCGGCCCTTCGGGGAGGCCCCGAGAGAGCTGCGC CTGCGGGCCACCCGTGCCTTCGTGGCTGCTCGCTCCTTTGTGCAGGGCCTGGGCGTGGCCAGCGACGTGG TCCGGAAAGTGGCTCAGGTCCCCCTGGGCCCGGAGTGCTCGAGAGCTGTCATGAAGCTGGTCTACTGTGC TCACTGCCTGGGAGTCCCCGGCGCCAGGCCCTGCCCTGACTATTGCCGAAATGTGCTCAAGGGCTGCCTT GCCAACCAGGCCGACCTGGACGCCGAGTGGAGGAACCTCCTGGACTCCATGGTGCTCATCACCGACAAGT TCTGGGGTACATCGGGTGTGGAGAGTGTCATCGGCAGCGTGCACACGTGGCTGGCGGAGGCCATCAACGC CCTCCAGGACAACAGGGACACGCTCACGGCCAAGGTCATCCAGGGCTGCGGGAACCCCAAGGTCAACCCC CAGGGCCCTGGGCCTGAGGAGAAGCGGCGCCGGGGCAAGCTGGCCCCGCGGGAGAGGCCACCTTCAGGCA CGCTGGAGAAGCTGGTCTCTGAAGCCAAGGCCCAGCTCCGCGACGTCCAGGACTTCTGGATCAGCCTCCC AGGGACACTGTGCAGTGAGAAGATGGCCCTGAGCACTGCCAGTGATGACCGCTGCTGGAACGGGATGGCC AGAGGCCGGTACCTCCCCGAGGTCATGGGTGACGGCCTGGCCAACCAGATCAACAACCCCGAGGTGGAGG TGGACATCACCAAGCCGGACATGACCATCCGGCAGCAGATCATGCAGCTGAAGATCATGACCAACCGGCT GCGCAGCGCCTACAACGGCAACGACGTGGACTTCCAGGACGCCAGTGACGACGGCAGCGGCTCGGGCAGC GGTGATGGCTGTCTGGATGACCTCTGCGGCCGGAAGGTCAGCAGGAAGAGCTCCAGCTCCCGGACGCCCT TGACCCATGCCCTCCCAGGCCTGTCAGAGCAGGAAGGACAGAAGACCTCGGCTGCCAGCTGCCCCCAGCC CCCGACCTTCCTCCTGCCCCTCCTCCTCTTCCTGGCCCTTACAGTAGCCAGGCCCCGGTGGCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208602 representing NM_002081
Red=Cloning site Green=Tags(s) MELRARGWWLLCAAAALVACARGDPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCT SEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFGELYTQNARA FRDLYSELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLLPDDYLDCLGKQAEALRPFGEAPRELR LRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCL ANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNP QGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMA RGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGS GDGCLDDLCGRKVSRKSSSSRTPLTHALPGLSEQEGQKTSAASCPQPPTFLLPLLLFLALTVARPRWR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002081 |
ORF Size | 1674 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002081.1, NP_002072.1 |
RefSeq Size | 3692 bp |
RefSeq ORF | 1677 bp |
Locus ID | 2817 |
UniProt ID | P35052 |
Domains | Glypican |
Protein Families | Druggable Genome |
Gene Summary | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208602 | GPC1 (Myc-DDK-tagged)-Human glypican 1 (GPC1) |
CNY 4,152.00 |
|
RC208602L3 | Lenti ORF clone of Human glypican 1 (GPC1), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC208602L4 | Lenti ORF clone of Human glypican 1 (GPC1), mGFP tagged |
CNY 6,552.00 |
|
SC321494 | GPC1 (untagged)-Human glypican 1 (GPC1) |
CNY 4,160.00 |