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ARIH1 (NM_005744) Human Tagged ORF Clone
CAT＃: RG208707
- TrueORF®
ARIH1 (tGFP-tagged) - Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1)
ORF Plasmid: DDK
"NM_005744" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARI; HARI; HHARI; UBCH7BP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208707 representing NM_005744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGACGAGGGCTACAACTACGAGTTCGACGAGGACGAGGAGTGCAGTGAGGAGGACAGCGGCG CCGAGGAGGAGGAGGACGAAGACGACGACGAGCCGGACGATGATACCCTGGATCTGGGCGAGGTGGAGCT GGTGGAGCCCGGGCTGGGCGTCGGCGGGGAGCGGGACGGACTGCTGTGCGGGGAGACGGGCGGTGGCGGC GGCAGCGCTCTGGGGCCCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGTGGTGGTGGCGGGCCGGGGCATG AGCAGGAGGAGGATTACCGCTACGAGGTGCTCACGGCCGAGCAGATTCTACAACACATGGTGGAATGTAT CCGGGAGGTCAACGAGGTCATCCAGAATCCAGCAACTATCACAAGAATACTCCTTAGCCACTTCAATTGG GATAAAGAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGCTGAGTGTCATGTAA TTAATCCAAGTAAAAAGTCTCGAACACGCCAGATGAATACAAGGTCATCAGCACAGGATATGCCTTGTCA GATCTGCTACTTGAACTACCCTAACTCGTATTTCACTGGCCTTGAATGTGGACATAAGTTTTGTATGCAG TGCTGGAGTGAATATTTAACTACCAAAATAATGGAAGAAGGCATGGGTCAGACTATTTCGTGTCCTGCTC ATGGTTGTGATATCTTAGTGGATGACAACACAGTTATGCGCCTGATCACAGATTCAAAAGTTAAATTAAA GTATCAGCATTTAATAACAAATAGCTTTGTAGAGTGCAATCGACTGTTAAAGTGGTGTCCTGCCCCAGAT TGCCACCATGTTGTTAAAGTCCAATATCCTGATGCTAAACCTGTTCGCTGCAAATGTGGGCGCCAATTTT GCTTTAACTGTGGAGAAAATTGGCATGATCCTGTTAAATGTAAGTGGTTAAAGAAATGGATTAAAAAGTG TGATGATGACAGTGAAACCTCCAATTGGATTGCAGCCAACACAAAGGAATGTCCCAAATGCCATGTCACA ATTGAGAAGGATGGTGGTTGTAATCACATGGTCTGTCGTAACCAGAATTGTAAAGCAGAGTTTTGCTGGG TGTGTCTTGGCCCATGGGAACCACATGGATCTGCCTGGTACAACTGTAACCGCTATAATGAGGATGATGC AAAGGCAGCAAGAGATGCACAGGAGCGATCTAGGGCAGCCCTGCAGAGGTACCTGTTCTACTGTAATCGC TATATGAACCACATGCAGAGCCTGCGCTTTGAGCACAAACTATATGCTCAGGTGAAACAGAAAATGGAGG AGATGCAGCAGCACAACATGTCCTGGATTGAGGTGCAGTTCCTGAAGAAGGCAGTTGATGTCCTCTGCCA GTGTCGTGCCACACTCATGTACACTTATGTCTTCGCTTTCTACCTCAAAAAGAATAACCAGTCCATTATC TTTGAGAATAACCAAGCAGATCTAGAGAATGCCACAGAGGTGCTCTCGGGCTACCTTGAACGAGATATTT CCCAAGATTCTCTGCAGGATATAAAGCAGAAAGTACAAGACAAGTACAGATACTGTGAGAGTCGACGAAG GGTTTTGTTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTGAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208707 representing NM_005744
Red=Cloning site Green=Tags(s) MDSDEGYNYEFDEDEECSEEDSGAEEEEDEDDDEPDDDTLDLGEVELVEPGLGVGGERDGLLCGETGGGG GSALGPGGGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNW DKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQ CWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPD CHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVT IEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNR YMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSII FENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005744 |
| ORF Size | 1671 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005744.5 |
| RefSeq Size | 2178 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 25820 |
| UniProt ID | Q9Y4X5 |
| Domains | IBR |
| Protein Families | Druggable Genome |
| Gene Summary | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208707 | ARIH1 (Myc-DDK-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 3,990.00 |
|
| RC208707L1 | Lenti-ORF clone of ARIH1 (Myc-DDK-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 8,624.00 |
|
| RC208707L2 | Lenti-ORF clone of ARIH1 (mGFP-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 5,890.00 |
|
| RC208707L3 | Lenti-ORF clone of ARIH1 (Myc-DDK-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 5,890.00 |
|
| RC208707L4 | Lenti-ORF clone of ARIH1 (mGFP-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 5,890.00 |
|
| SC116547 | ARIH1 (untagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 6,232.00 |
