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ARIH1 (NM_005744) Human Untagged Clone
CAT＃: SC116547
ARIH1 (untagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1)
CNY 6,232.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARI; HARI; HHARI; UBCH7BP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005744 edited
GAATTCGGCACGAGGCAGCCGTCAAACGCCAACCGCCGCTCCTGGGGAGGAGCCGCGGCT CGCGGGGCCGGAGCCAGGCCTGCGTCCGGACATCAGCCGGAGCCGGAGCGAGAGCCGGGG CCTCGGCGTCCCCGCCCTCTCCCCGCCTCGGCCAGCGTCCGCCGGGCCCCCGCGCGTCGC GCCATGGACTCGGACGAGGGCTACAACTACGAGTTCGACGAGGACGAGGAGTGCAGTGAG GAGGACAGCGGCGCCGAGGAGGAGGAGGACGAAGACGACGACGAGCCGGACGATGATACC CTGGATCTGGGCGAGGTGGAGCTGGTGGAGCCCGGGCTGGGCGTCGGCGGGGAGCGGGAC GGACTGCTGTGCGGGGAGACGGGCGGTGGCGGCGGCAGCGCTCTGGGGCCCGGCGGTGGC GGCGGCGGCGGCGGCGGCGGTGGTGGTGGCGGGCCGGGGCATGAGCAGGAGGAGGATTAC CGCTACGAGGTGCTCACGGCCGAGCAGATTCTACAACACATGGTGGAATGTATCCGGGAG GTCAACGAGGTCATCCAGAATCCAGCAACTATCACAAGAATACTCCTTAGCCACTTCAAT TGGGATAAAGAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGCT GAGTGTCATGTAATTAATCCAAGTAAAAAGTCTCGAACACGCCAGATGAATACAAGGTCA TCAGCACAGGATATGCCTTGTCAGATCTGCTACTTGAACTACCCTAACTCGTATTTCACT GGCCTTGAATGTGGACATAAGTTTTGTATGCAGTGCTGGAGTGAATATTTAACTACCAAA ATAATGGAAGAAGGCATGGGTCAGACTATTTCGTGTCCTGCTCATGGTTGTGATATCTTA GTGGATGACAACACAGTTATGCGCCTGATCACAGATTCAAAAGTTAAATTAAAGTATCAG CATTTAATAACAAATAGCTTTGTAGAGTGCAATCGACTGTTAAAGTGGTGTCCTGCCCCA GATTGCCACCATGTTGTTAAAGTCCAATATCCTGATGCTAAACCTGTTCGCTGCAAATGT GGGCGCCAATTTTGCTTTAACTGTGGAGAAAATTGGCATGATCCTGTTAAATGTAAGTGG TTAAAGAAATGGATTAAAAAGTGTGATGATGACAGTGAAACCTCCAATTGGATTGCAGCC AACACAAAGGAATGTCCCAAATGCCATGTCACAATTGAGAAGGATGGTGGTTGTAATCAC ATGGTCTGTCGTAACCAGAATTGTAAAGCAGAGTTTTGCTGGGTGTGTCTTGGCCCATGG GAACCACATGGATCTGCCTGGTACAACTGTAACCGCTATAATGAGGATGATGCAAAGGCA GCAAGAGATGCACAGGAGCGATCTAGGGCAGCCCTGCAGAGGTACCTGTTCTACTGTAAT CGCTATATGAACCACATGCAGAGCCTGCGCTTTGAGCACAAACTATATGCTCAGGTGAAA CAGAAAATGGAGGAGATGCAGCAGCACAACATGTCCTGGATTGAGGTGCAGTTCCTGAAG AAGGCAGTTGATGTCCTCTGCCAGTGTCGTGCCACACTCATGTACACTTATGTCTTCGCT TTCTACCTCAAAAAGAATAACCAGTCCATTATCTTTGAGAATAACCAAGCAGATCTAGAG AATGCCACAGAGGTGCTCTCGGGCTACCTTGAACGAGATATTTCCCAAGATTCTCTGCAG GATATAAAGCAGAAAGTACAAGACAAGTACAGATACTGTGAGAGTCGACGAAGGGTTTTG TTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTGAGGACTGAGAA TGGCCCTGCATAAAATGAACTCTGAAAACTTTACCATCTAGAGTGCTCATGCAATTAAAA CAAAACAAACACAAACAAGGAGGCACTAAGCCTATTCTGACACCACTGGTCTGTAGTACC AGAATTGTTTTGTTAATGGAAAGTTTAAGTAAATTATATTGTAATAAAAAGGTAGATAAA CCATTGTACAACAGTATTCTAGGCCGCCAACAAAAGTGTGACAGACACACTAAAAGCCCT CCAACTTTAACTTGTAACGTAGCTTCATTCTCAAAGCTGACTCCTTTTTTTTCTTTTTCC TTTTCCTGAGTGTAGTACAGTTAAAATTTCAAACAGCTCCTTGACACTGCTTTTCATGTT CAAACCAGCCATTTTGTTGTACTTTGGTAAAGGACCTCTTCCCCTTCCTCCCCTACACAT ACAGATACACCCACACACAGACTGACTCTCTTTCTCTCATACCCCAAGGTCATGAGTGAA TGATGCTTAGTTCCTTGTAAAGAAAATCTTGGGATGGGGAAAGGGGTAGGCAGCAAGAGG ATTCAACAAACGAAAAACATAAAAACTTTGTATATGACTTTTAAAACAAGAGGACAACAC AGTATTTTTCAAAATTGTATATAGCGCATATGCATGGACAAAGCAAGCGTGGCACGTGTT TGCATAATGTTTAATTACAAAAAAATATTTATTCTTTAAAAATCTTCAAAAAAAAAAAAA AAAAACTCGAC >OriGene 5' read for NM_005744 unedited
GGTTCCGACGACTTAGGATAGGGNTGACNGCGTAATTCGTCACGAGGCAGCCGNCTAACG CCAACCGCCGCTCCTGGGGACGAGCCGCGGCTCGCGGGGCCGGAGCCAGGCCTGCGTCCG GACATCAGCCGGAGCCGGAGCGAGAGCCGGGGCCTCGGCGTCCCCGCCCTCTCCCCGCCT CGGCCAGCGTCCGCCGGGCCCCCGCGCGTCGCGCCATGGACTCGGACGAGGGCTACAACT ACGAGTTCGACGAGGACGAGGAGTGCAGTGAGGAGGACAGCGGCGCCGAGGAGGAGGAGG ACGAAGACGACGACGAGCCGGACGATGATACCCTGGATCTGGGCGAGGTGGAGCTGGTGG AGCCCGGGCTGGGCGTCGGCGGGGAGCGGGACGGACTGCTGTGCGGGGAGACGGGCGGTG GCGGCGGCAGCGCTCTGGGGCCCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGTGGTGGTG GCGGGCCGGGGCATGAGCAGGAGGAGGATTACCGCTACGAGGTGCTCACGGCCGAGCAGA TTCTACAACACATGGTGGAATGTATCCGGGAGGTCAACGAGGTCATCCAGAATCCAGCAA CTATCACAAGAATACTCCTTAGCCACTTCAATTGGGATAAAGAGAAGCTAATGGAAAGGT ACTTTGATGGAAACCTGGAGAAGCTCTTTGCTGAGTGTCATGTAATTAATCCAAGTGAAA AGTCTCGAACACGCCAGATGAATACAAGGTCATCAGCACAGGATATGCCTTGTCAGATCT GCTACTTGAACTACCCTCACTCGTATTTCACTGGCCTCTGATGTGGACATAAGTTTTGTA TGCAGTGCTGGAGTGAATATCTAACTACCAAATTATGGAAGAGGCATGGGTCAGACTATT TGNGGCCTGCTCATGGTTGGAATCTTAATGAAGACACCA >OriGene 3' genomic read for NM_005744 unedited
GTACATTATGGACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTGAAGATTT TAAAGAATAAATATTTTTTTGTAATTAAACATTATGCAAACACGTGCCACGCTTGCTTTG TCCATGCATATGCGCTATATACAATTTTGAAAAATACTGTGTTGTCCTCTTGTTTTAAAA GTCATATACAAAGTTTTTATGTTTTTCGTTTGTTGAATCCTCTTGCTGCCTACCCCTTTC CCCATCCCAAGATTTTCTTTACAAGGAACTAAGCATCATTCACTCATGACCTTGGGGTAT GAGAGAAAGAGAGTCAGTCTGTGTGTGGGTGTATCTGTATGTGTAGGGGAGGAAGGGGAA GAGGNNCNNNNCCNNNGNNCAACANAATGGCTGGNNTGAACATGAAAAGCAGGGTCAAGG AGCTGNTTGAAATTTTAACTGTACTACACTCAGGAAAAGGAAAAAGAAAAAAAAGGAGTC AGCTTTGAGAATGAAGCTACGTTACAAGTTAAAGTTGGAGGGCTTTTAGTGTGTCTGTCA CACTTTTGTTGGCGGCCTAACATACTGTTGTACAATGGGTTATCTACCTTTTTATTACAA TATGAATTACTTAAACTTTCCATTAACAAAACAATTCTGGGACTACATACCAGTGGTGTC AGAATAGGCTTAGTGCCTCCTTGTTTGGGTTTGGTTTGGTTTAAATGCGGGAGCACTTTT AGATGGGAAAGTTATTCGAATTTATTTTTATAGCTGGCCCTTTCTTCGTTCCTTCATGGA CTTCCGCCAAATCTTTTCTAAAGGCCTGGATGCCAAGGCTGGACCTAAAACCTTTCATCG ACTATTCAAGAGGCAAGAACTTGGTTTGTCTCTTTTCTGCCTTTAATTCCGGACGGGCAA GTTCGGGAAAAAATATTGGTAAAGAGGGAGCCTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005744 |
| Insert Size | 2750 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005744.2, NP_005735.1 |
| RefSeq Size | 2178 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 25820 |
| UniProt ID | Q9Y4X5 |
| Domains | IBR |
| Protein Families | Druggable Genome |
| Gene Summary | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208707 | ARIH1 (Myc-DDK-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 3,990.00 |
|
| RC208707L1 | Lenti-ORF clone of ARIH1 (Myc-DDK-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 8,624.00 |
|
| RC208707L2 | Lenti-ORF clone of ARIH1 (mGFP-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 5,890.00 |
|
| RC208707L3 | Lenti-ORF clone of ARIH1 (Myc-DDK-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 5,890.00 |
|
| RC208707L4 | Lenti-ORF clone of ARIH1 (mGFP-tagged)-Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 5,890.00 |
|
| RG208707 | ARIH1 (tGFP-tagged) - Human ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) |
CNY 4,370.00 |
