hnRNP L (HNRNPL) (NM_001005335) Human Tagged ORF Clone

CAT#: RG209067

  • TrueORF®

HNRNPL (tGFP-tagged) - Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001005335" in other vectors (4)

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CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


HNRNPL mouse monoclonal antibody, clone OTI9G7 (formerly 9G7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms hnRNP-L; HNRPL; P/OKcl.14
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG209067 representing NM_001005335
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGATGGCGGCGGCGGGCGGCGGAGGCGGCGGTGGCCGCTACTACGGCGGCGGCAGTGAGGGCG
GCCGGGCCCCTAAGCGGCTCAAGACTGACAACGCCGGCGACCAGCACGGAGGCGGCGGCGGTGGCGGTGG
AGGAGCCGGGGCGGCGGGCGGCGGCGGCGGTGGGGAGAACTACGATGACCCGCACAAAACCCCTGCCTCC
CCAGTTGTCCACATCAGGGGCCTGATTGACGGTGTGGTGGAAGCAGACCTTGTGGAGGCCTTGCAGGAGT
TTGGACCCATCAGCTATGTGGTGGTAATGCCTAAAAAGAGACAAGCACTGGTGGAGTTTGAAGATGTGTT
GGGGGCTTGCAACGCAGTGAACTACGCAGCCGACAACCAAATATACATTGCTGGTCACCCAGCTTTTGTC
AACTACTCTACCAGCCAGAAGATCTCCCGCCCTGGGGACTCGGATGACTCCCGGAGCGTGAACAGTGTGC
TTCTCTTTACCATCCTGAACCCCATTTATTCGATCACCACGGATGTTCTTTACACTATCTGTAATCCTTG
TGGCCCTGTCCAGAGAATTGTCATTTTCAGGAAGAATGGAGTTCAGGCGATGGTGGAATTTGACTCAGTT
CAAAGTGCCCAGCGGGCCAAGGCCTCTCTCAATGGGGCTGATATCTATTCTGGCTGTTGCACTCTGAAGA
TCGAATACGCAAAGCCTACACGCTTGAATGTGTTCAAGAATGATCAGGATACTTGGGACTACACAAACCC
CAATCTCAGTGGACAAGGTGACCCTGGCAGCAACCCCAACAAACGCCAGAGGCAGCCCCCTCTCCTGGGA
GATCACCCCGCAGAATATGGAGGGCCCCACGGTGGGTACCACAGCCATTACCATGATGAGGGCTACGGGC
CCCCCCCACCTCACTACGAAGGGAGAAGGATGGGTCCACCAGTGGGGGGTCACCGTCGGGGCCCAAGTCG
CTACGGCCCCCAGTATGGGCACCCCCCACCCCCTCCCCCACCACCCGAGTATGGCCCTCACGCCGACAGC
CCTGTGCTCATGGTCTATGGCTTGGATCAATCTAAGATGAACTGTGACCGAGTCTTCAATGTCTTCTGCT
TATATGGCAATGTGGAGAAGGTGAAATTCATGAAAAGCAAGCCGGGGGCCGCCATGGTGGAGATGGCTGA
TGGCTACGCTGTAGACCGGGCCATTACCCACCTCAACAACAACTTCATGTTTGGGCAGAAGCTGAATGTC
TGTGTCTCCAAGCAGCCAGCCATCATGCCTGGTCAGTCATACGGGTTGGAAGACGGGTCTTGCAGTTACA
AAGACTTCAGTGAATCCCGGAACAATCGGTTCTCCACCCCAGAGCAGGCAGCCAAGAACCGCATCCAGCA
CCCCAGCAACGTGCTGCACTTCTTCAACGCCCCGCTGGAGGTGACCGAGGAGAACTTCTTTGAGATCTGC
GATGAGCTGGGAGTGAAGCGGCCATCTTCTGTGAAAGTATTCTCAGGCAAAAGTGAGCGCAGCTCCTCTG
GACTGCTGGAGTGGGAATCCAAGAGCGATGCCCTGGAGACTCTGGGCTTCCTGAACCATTACCAGATGAA
AAACCCAAATGGTCCATACCCTTACACTCTGAAGTTGTGTTTCTCCACTGCTCAGCACGCCTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG209067 representing NM_001005335
Red=Cloning site Green=Tags(s)

MVKMAAAGGGGGGGRYYGGGSEGGRAPKRLKTDNAGDQHGGGGGGGGGAGAAGGGGGGENYDDPHKTPAS
PVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFV
NYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSV
QSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLG
DHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADS
PVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNV
CVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEIC
DELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001005335
ORF Size 1674 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001005335.1, NP_001005335.1
RefSeq Size 1895 bp
RefSeq ORF 1371 bp
Locus ID 3191
UniProt ID P14866
Gene Summary Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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