sphingosine 1 phosphate phosphatase 1 (SGPP1) (NM_030791) Human Tagged ORF Clone
CAT#: RG209497
- TrueORF®
SGPP1 (tGFP-tagged) - Human sphingosine-1-phosphate phosphatase 1 (SGPP1)
ORF Plasmid: DDK
"NM_030791" in other vectors (7)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SPP-1; SPPase1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209497 representing NM_030791
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGAGGCAGCGCCTGGCCCAGCTGGTTGGCCGTCTGCAGGACCCGCAGAAAGTGGCCCGTTTCC AGCGGCTGTGCGGGGTGGAAGCGCCGCCGCGCCGCTCAGCAGACCGGAGGGAGGATGAGAAAGCGGAGGC GCCTCTCGCCGGAGACCCTCGACTGCGAGGGCGGCAGCCAGGGGCGCCTGGAGGCCCCCAGCCTCCCGGG AGCGACCGCAATCAGTGCCCGGCCAAGCCGGACGGCGGCGGCGCCCCCAACGGCGTGCGGAACGGGCTGG CGGCCGAGCTGGGCCCGGCCTCGCCGCGGCGCGCGGGCGCTCTGCGCCGCAACTCGCTGACGGGCGAGGA GGGCCAGCTGGCCCGCGTGAGCAACTGGCCGCTCTACTGCCTGTTCTGCTTCGGCACGGAGCTGGGCAAC GAACTCTTCTACATCCTGTTCTTCCCCTTCTGGATCTGGAACCTGGACCCTCTGGTGGGCCGGAGGCTCG TGGTCATCTGGGTGCTGGTCATGTACCTGGGCCAGTGCACCAAGGACATCATCCGCTGGCCGAGGCCCGC CTCGCCGCCCGTGGTCAAGTTGGAGGTCTTCTACAACTCTGAGTACAGCATGCCCTCCACCCATGCCATG TCCGGCACCGCCATCCCCATTTCTATGGTCCTCCTCACCTATGGCCGCTGGCAGTACCCTCTTATATATG GACTGATTCTTATTCCCTGCTGGTGTTCTCTAGTTTGCCTAAGTAGAATTTACATGGGAATGCACTCTAT TCTGGATATTATTGCTGGATTCCTATATACCATTTTAATCTTAGCTGTCTTCTATCCATTTGTGGACCTG ATTGACAACTTCAACCAAACTCACAAATATGCTCCATTCATCATCATCGGGCTTCATTTAGCTTTGGGGA TCTTTTCTTTCACTCTTGACACCTGGAGCACATCCCGAGGAGACACAGCCGAGATACTAGGAAGTGGTGC TGGAATTGCATGTGGATCTCATGTTACTTATAACATGGGTCTAGTATTAGATCCTTCTCTAGATACATTA CCTTTAGCTGGGCCCCCCATTACTGTGACTCTGTTTGGAAAAGCCATATTGCGGATCCTCATAGGGATGG TATTTGTACTAATAATCAGAGATGTAATGAAAAAGATCACCATTCCTTTAGCCTGCAAAATCTTCAATAT ACCGTGTGATGATATTCGAAAAGCAAGACAGCACATGGAAGTTGAACTTCCTTATCGGTATATTACCTAT GGAATGGTTGGTTTCTCCATCACATTTTTTGTTCCTTACATATTTTTCTTTATTGGTATCTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209497 representing NM_030791
Red=Cloning site Green=Tags(s) MSLRQRLAQLVGRLQDPQKVARFQRLCGVEAPPRRSADRREDEKAEAPLAGDPRLRGRQPGAPGGPQPPG SDRNQCPAKPDGGGAPNGVRNGLAAELGPASPRRAGALRRNSLTGEEGQLARVSNWPLYCLFCFGTELGN ELFYILFFPFWIWNLDPLVGRRLVVIWVLVMYLGQCTKDIIRWPRPASPPVVKLEVFYNSEYSMPSTHAM SGTAIPISMVLLTYGRWQYPLIYGLILIPCWCSLVCLSRIYMGMHSILDIIAGFLYTILILAVFYPFVDL IDNFNQTHKYAPFIIIGLHLALGIFSFTLDTWSTSRGDTAEILGSGAGIACGSHVTYNMGLVLDPSLDTL PLAGPPITVTLFGKAILRILIGMVFVLIIRDVMKKITIPLACKIFNIPCDDIRKARQHMEVELPYRYITY GMVGFSITFFVPYIFFFIGIS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030791 |
ORF Size | 1323 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030791.4 |
RefSeq Size | 3323 bp |
RefSeq ORF | 1326 bp |
Locus ID | 81537 |
UniProt ID | Q9BX95 |
Domains | acidPPc |
Protein Families | Druggable Genome, Phosphatase, Transmembrane |
Protein Pathways | Sphingolipid metabolism |
Gene Summary | Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209497 | SGPP1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 5,488.00 |
|
RC209497L1 | Lenti-ORF clone of SGPP1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 7,888.00 |
|
RC209497L2 | Lenti-ORF clone of SGPP1 (mGFP-tagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 5,890.00 |
|
RC209497L3 | Lenti-ORF clone of SGPP1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 5,890.00 |
|
RC209497L4 | Lenti-ORF clone of SGPP1 (mGFP-tagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 5,890.00 |
|
SC107997 | SGPP1 (untagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 5,488.00 |
|
SC322734 | SGPP1 (untagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1) |
CNY 5,488.00 |
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