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USP25 (NM_013396) Human Tagged ORF Clone
CAT＃: RG209755
- TrueORF®
USP25 (tGFP-tagged) - Human ubiquitin specific peptidase 25 (USP25)
ORF Plasmid: DDK
"NM_013396" in other vectors (6)
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CNY 8,944.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | USP21 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209755 representing NM_013396
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAAC TGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTT GGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTAC CAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATC TCACTGGAGATGATAAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGGGCATT CAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCAGAGAAT AAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGAC AGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATTCAGTCATT ATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCC CGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCACTTCTTGTTG GTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTCAAATCAAATGA CTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATG AAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTATGGCAGATTCC TGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTTGGTCAGTACCCACTTCAGGT CAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAAATTGAGTCTTTACAT TCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAAT TGTCAAGATTTGAATTTAATCAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCA AGTTTTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAG AGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTAAGCTATGGTTCCGGTCCCA AACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCTGTTTGCACTTC TCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCATACCATCACAGACATTACCAAGCACA ACAGAACAACAGGGAGCCCTATCTTCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCAT CGAGATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACC AAGGCACATAACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAA AATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACTCTGACA AATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAAGGCCAAGCTAATGCTGGGCA CTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAA TCATCATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTATAGAAATGCCAGTGCATACTGTTTAATGT ACATAAATGATAAGGCACAGTTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGG TATAGAAACATTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTA GAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAGAAATTGAGAG AGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAG TGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAGGCATCACATGAGCATGAAGAT AAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAG AAGACACCCCACCAGAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACC AAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT CACAACATAATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAAT ATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCATAATTGGGCTAGAAAA TTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGCTTATCAGAATAACAAAGAACTC TTGTCTAAAGGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAA AATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTAT CATGAATGAGTTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTA GCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACACCTCCAAGAAA AGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAA GTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGTCGAACT CCTGCTGATGGAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209755 representing NM_013396
Red=Cloning site Green=Tags(s) MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYY QTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAEN KACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLP RNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQM KAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLH SENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIK RLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPST TEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIE NDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTK SSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKEL EEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHED KSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERC HNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKEL LSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDIL AVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRT PADGR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013396 |
| ORF Size | 3165 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013396.6 |
| RefSeq Size | 4968 bp |
| RefSeq ORF | 3168 bp |
| Locus ID | 29761 |
| UniProt ID | Q9UHP3 |
| Domains | UCH, UIM |
| Protein Families | Protease |
| Gene Summary | Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209755 | USP25 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 25 (USP25) |
CNY 7,344.00 |
|
| RC209755L1 | Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), Myc-DDK-tagged |
CNY 9,744.00 |
|
| RC209755L2 | Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), mGFP tagged |
CNY 12,450.00 |
|
| RC209755L3 | Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), Myc-DDK-tagged |
CNY 9,744.00 |
|
| RC209755L4 | Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), mGFP tagged |
CNY 9,744.00 |
|
| SC115248 | USP25 (untagged)-Human ubiquitin specific peptidase 25 (USP25) |
CNY 7,352.00 |
