USP25 (NM_013396) Human Untagged Clone

Name: USP25 (NM_013396) Human Untagged Clone
SKU: SC115248
Price: 7352.00

CAT＃: SC115248

USP25 (untagged)-Human ubiquitin specific peptidase 25 (USP25)

Datasheet

SDS

"NM_013396" in other vectors (6)

CNY 7,352.00

货期*

现货

规格

Cited in 2 publications.

Product images

经常一起买 (4)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA810971

USP25 mouse monoclonal antibody,clone OTI6H4

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	USP21
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC115248 sequence for NM_013396 edited (data generated by NextGen Sequencing) ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTT TTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTG AAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAG ACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATAC ATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGAT GATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGGGCATTCAGGGAGACT GGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCAGAGAAT AAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGAT AGAAAAAGACAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTT AGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTAC AAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCT TTTATGCGTGAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTT GATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTCAAATCAAATGACTCACAGCAG CAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATG AAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTAT GGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTT GGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCA ATGATTGAAGGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAG CATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAAT CAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATAT TTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAG AGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTAAGCTATGGT TCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCA AGTAAACCTGTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGT TCCATACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCTTCAGAA CTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAA CCATTTACTCAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATA ACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAA AATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATG TACTCTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAA GGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGG ATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCT TTTGGTGGTTATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAG TTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACA TTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTA GAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAG AAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGAGACCCAGAATAT CTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATA ATTACCAAGGCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCA ATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCAGAAACC GATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATT ATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT CACAACATAATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAAC TTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTAT CTCATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTC ATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGACATGAT GAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCA GAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAG TTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTA GCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACAC CTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAA AGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTT GCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGATAA Clone variation with respect to NM_013396.3 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_013396 unedited AGGTCAGAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGCCCG CGGACCGGCAGCAAAGGACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCC GTGCCCCGGCGTCCTCCGCCCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGG CGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCGTTTCGCCGCCT GCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCT GTCCTCCCAGGCCGTCCGCGCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCG GCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCCGCGGGGGCCAT GACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTT GAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAA GGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGAC CCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACAT CAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGATGA TCCTCAGAGAGCAATTGCCTTGAGTTTGGCCCGATCAAACAGGGCATTCAGGCAGACTGG AATAACTGATGACGAACAAGCCCTTACCAGAGTTCTTTGAACCAGCATACCAGAGAATAA GCCTGTTTTGAAGAAGACCCTACAGAAGTTGTGGAGGGATTCTCCAAACCCTTATGATAG A 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_013396 unedited ATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGCCATAAATAA AACTTTATTCTTTATTTCATTTACAAGCTACCAAATATTATGTATCGTACACAGTGCTGA ACACTTAAATGGCTGTAGTCATGGAAGGATCCAGACTGAATGGAAAGCTGTTGAGAAAGA AAAGATAAAAGCAAAGTAATACTGCAACAGGAAGGTGGCAAAAGCATAGTTTTGCCATAA TAAAATCAATTAGATTTGTGATTATACATCAGTTCCGGTTAAAATGTCTGAGCGCCATGC GATTTTCAGCTTTATTGTCTGCAGTCTGACTAAAGTCTGTATAGTCATTTTGTCTTTTGC AGTTATTAAAATAAAAAAAAGTTAAAAACTATAGCAGCAACAAGCAAACCCTGTGACAGG AAGGCAAGGGTTAAGAACTAAAAAGAGTTTATACAGTGTGTTCAGGGAAAGTGTGCAGTT TATCTTCCATCAGCAGGAGTTCGACTGAGGGACAACATGATTCGGGCAAATCGCTCACAG AGTTCATGCGTGGAATATGAAGGTAACTTCGGTGGCTCATGGAAACTTTTATCTCCTAGA ACAATCAAGCAGTTTTGGCAAAAAATCTGTCCACTTTTTTTGGAAGTGTGGTTCCCTTTC TTGACCAAGGTAGGAACCCCATCGGATTCTCATATTTTTTACAGCTAAGATATCCTCTTT TTCCTTTTCATCCACCAGTATTAATGGCAAAAATGGGACAATTAACTTTATTCTTGATAA TTAACCCTTTGTTTCCTTCTTGAACCCCCCCAAATCCAAAATTTTGGCGTTTGCCCTTTA ATTTACAAAAATTTCTTTTTTATGCGACAAAATTCTTATCATTCCCCTGTTTACACCTT
Restriction Sites	NotI-NotI
ACCN	NM_013396
Insert Size	4500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013396.3, NP_037528.3
RefSeq Size	4968 bp
RefSeq ORF	3168 bp
Locus ID	29761
UniProt ID	Q9UHP3
Domains	UCH, UIM
Protein Families	Protease
Gene Summary	Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (USP25a) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this cDNA Clones has been cited in the following citations:
Cigarette smoke extract modulates Pseudomonas aeruginosa bacterial load via USP25/HDAC11 axis in lung epithelial cells ,Long, C;Lai, Y;Li, T;Nyunoya, T;Zou, C;, Am. J. Physiol. Lung Cell Mol. Physiol. ,PubMed ID 31746627 [USP25]
LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells ,null, Biochimica et biophysica acta. Gene regulatory mechanisms ,PubMed ID 30745998 [USP25]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC209755	USP25 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 25 (USP25)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,344.00
RC209755L1	Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,744.00
RC209755L2	Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,450.00
RC209755L3	Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,744.00
RC209755L4	Lenti ORF clone of Human ubiquitin specific peptidase 25 (USP25), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,744.00
RG209755	USP25 (tGFP-tagged) - Human ubiquitin specific peptidase 25 (USP25)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,944.00

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