United States
Germany
Japan
United Kingdom
China
ACOT12 (NM_130767) Human Tagged ORF Clone
CAT＃: RG210445
- TrueORF®
ACOT12 (tGFP-tagged) - Human acyl-CoA thioesterase 12 (ACOT12)
ORF Plasmid: DDK
"NM_130767" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cach; CACH-1; STARD15; THEAL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210445 representing NM_130767
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCCGGCGCCCGGCGAGGTGGTCATGAGCCAAGCCATCCAGCCGGCGCACGCCACTGCGCGCG GCGAGCTGAGCGCGGGGCAGCTGCTCAAGTGGATCGACACCACCGCCTGCCTGGCGGCTGAGAAACATGC TGGAGTTTCCTGCGTTACAGCCTCAGTGGATGACATACAGTTTGAGGAGACAGCTAGAGTTGGACAAGTT ATAACCATCAAAGCAAAAGTTACTAGAGCATTCAGCACAAGCATGGAGATCAGTATCAAGGTCATGGTAC AGGATATGCTCACTGGCATTGAGAAGCTTGTTAGTGTGGCTTTCTCCACATTTGTAGCCAAACCAGTTGG AAAAGAAAAGATTCATTTAAAACCAGTCACACTTCTAACTGAACAAGATCATGTGGAACATAATCTGGCT GCTGAGAGAAGGAAAGTTCGATTACAACATGAAGATACCTTTAACAATTTAATGAAGGAAAGTAGCAAAT TTGATGATCTCATTTTTGATGAAGAGGAAGGAGCGGTTTCCACAAGGGGCACCTCCGTTCAGAGCATTGA ACTGGTCCTCCCACCCCATGCAAACCATCACGGAAATACATTTGGTGGCCAGATTATGGCGTGGATGGAG ACAGTGGCTACTATTTCTGCAAGCCGCCTGTGTTGGGCTCATCCCTTTCTGAAGTCCGTAGATATGTTTA AGTTCCGGGGACCATCTACAGTTGGAGATCGTCTTGTCTTCACTGCCATTGTCAACAATACATTTCAGAC CTGTGTTGAAGTTGGAGTTCGCGTGGAGGCCTTTGACTGTCAGGAATGGGCCGAGGGCCGAGGGCGTCAC ATCAACAGTGCTTTTCTCATTTACAATGCTGCTGATGATAAGGAAAATCTCATCACGTTTCCCAGAATCC AACCCATTTCAAAGGATGATTTCAGACGCTATCGGGGAGCTATTGCACGCAAGCGAATTCGCCTAGGCAG AAAATATGTTATTTCCCACAAAGAAGAGGTTCCACTTTGCATACACTGGGATATCAGCAAGCAGGCATCC CTGAGTGACAGCAATGTGGAGGCCCTCAAAAAACTGGCAGCCAAAAGGGGTTGGGAGGTTACCAGCACTG TGGAAAAGATAAAAATATATACTCTGGAAGAGCATGATGTTTTATCTGTTTGGGTTGAAAAGCACGTGGG AAGTCCAGCACATTTGGCTTATCGTCTCTTGTCTGACTTTACAAAGCGACCTTTGTGGGACCCCCATTTT GTGTCCTGTGAAGTCATAGACTGGGTGAGTGAAGATGATCAGCTGTATCACATCACCTGTCCTATACTGA ATGATGACAAACCCAAAGACTTGGTAGTACTCGTATCACGAAGAAAACCCCTCAAAGATGGTAACACTTA CACAGTGGCAGTGAAGTCGGTCATTTTGCCATCGGTCCCCCCGTCTCCACAGTACATCAGAAGTGAAATC ATATGTGCCGGATTTCTCATCCATGCTATTGACAGCAATTCATGCATCGTATCTTACTTTAACCATATGT CTGCTAGCATCCTTCCTTACTTTGCTGGAAATCTTGGTGGCTGGTCAAAATCCATTGAAGAAACAGCAGC CTCTTGTATACAGTTCTTAGAGAATCCTCCTGATGATGGGTTTGTAAGCACATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210445 representing NM_130767
Red=Cloning site Green=Tags(s) MERPAPGEVVMSQAIQPAHATARGELSAGQLLKWIDTTACLAAEKHAGVSCVTASVDDIQFEETARVGQV ITIKAKVTRAFSTSMEISIKVMVQDMLTGIEKLVSVAFSTFVAKPVGKEKIHLKPVTLLTEQDHVEHNLA AERRKVRLQHEDTFNNLMKESSKFDDLIFDEEEGAVSTRGTSVQSIELVLPPHANHHGNTFGGQIMAWME TVATISASRLCWAHPFLKSVDMFKFRGPSTVGDRLVFTAIVNNTFQTCVEVGVRVEAFDCQEWAEGRGRH INSAFLIYNAADDKENLITFPRIQPISKDDFRRYRGAIARKRIRLGRKYVISHKEEVPLCIHWDISKQAS LSDSNVEALKKLAAKRGWEVTSTVEKIKIYTLEEHDVLSVWVEKHVGSPAHLAYRLLSDFTKRPLWDPHF VSCEVIDWVSEDDQLYHITCPILNDDKPKDLVVLVSRRKPLKDGNTYTVAVKSVILPSVPPSPQYIRSEI ICAGFLIHAIDSNSCIVSYFNHMSASILPYFAGNLGGWSKSIEETAASCIQFLENPPDDGFVSTF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130767 |
| ORF Size | 1665 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130767.3 |
| RefSeq Size | 1820 bp |
| RefSeq ORF | 1668 bp |
| Locus ID | 134526 |
| UniProt ID | Q8WYK0 |
| Protein Pathways | Pyruvate metabolism |
| Gene Summary | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:16951743). Acyl-coenzyme A thioesterase 12/ACOT12 preferentially hydrolyzes acetyl-CoA (PubMed:16951743).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210445 | ACOT12 (Myc-DDK-tagged)-Human acyl-CoA thioesterase 12 (ACOT12) |
CNY 3,990.00 |
|
| RC210445L3 | Lenti ORF clone of Human acyl-CoA thioesterase 12 (ACOT12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210445L4 | Lenti ORF clone of Human acyl-CoA thioesterase 12 (ACOT12), mGFP tagged |
CNY 5,890.00 |
|
| SC305897 | ACOT12 (untagged)-Human acyl-CoA thioesterase 12 (ACOT12) |
CNY 4,144.00 |
