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PITRM1 (NM_014889) Human Tagged ORF Clone
CAT＃: RG210596
- TrueORF®
PITRM1 (tGFP-tagged) - Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: DDK
"NM_014889" in other vectors (6)
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CNY 11,400.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MP1; PreP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210596 representing NM_014889
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGCTGCGGCGGGCGGCAGGGCCTGTGTGTGCTGAGGCGGCTGAGCGGCGGACATGCACACCACA GAGCGTGGCGATGGAACAGTAACCGGGCTTGTGAGAGGGCTCTGCAGTATAAACTAGGAGACAAGATCCA TGGATTCACCGTAAACCAGGTGACATCTGTTCCCGAGCTGTTCCTGACTGCAGTGAAGCTCACCCATGAT GACACAGGAGCCAGGTATTTACACCTGGCCAGAGAAGACACGAATAATCTGTTCAGCGTGCAGTTCCGTA CCACTCCCATGGACAGTACTGGTGTTCCTCACATTCTTGAGCATACTGTCCTTTGTGGGTCTCAGAAATA TCCGTGCAGAGACCCTTTCTTCAAAATGTTGAACCGGTCCCTCTCCACGTTCATGAACGCCTTCACAGCT AGTGATTATACTGTGTATCCATTTTCCACACAAAATCCCAAGGACTTTCAGAATCTCCTCTCGGTGTATT TGGATGCCACCTTTTTCCCATGTTTACGCGAGCTGGATTTCTGGCAGGAAGGATGGCGGCTGGAACATGA GAATCCGAGCGACCCCCAGACGCCCTTGGTCTTTAAAGGAGTCGTCTTTAATGAGATGAAGGGAGCGTTT ACAGACAATGAGAGGATATTCTCCCAGCACCTTCAGAACAGACTTCTTCCCGACCACACGTACTCAGTGG TCTCCGGGGGTGACCCACTGTGCATCCCGGAGCTTACATGGGAGCAGCTTAAGCAGTTTCATGCCACTCA CTATCACCCAAGCAATGCTAGGTTCTTCACGTACGGTAATTTTCCATTAGAACAGCATCTGAAACAAATT CACGAGGAAGCACTGAGCAAATTCCAGAAAATTGAACCAAGCACCGTGGTGCCAGCTCAGACACCCTGGG ACAAGCCTAGGGAATTCCAGATAACATGTGGCCCGGATTCATTTGCTACAGATCCCTCTAAACAAACAAC CGTCAGCGTTAGCTTCCTCTTACCGGACATCACCGACACATTTGAAGCCTTCACATTAAGTCTTCTGTCT TCACTCTTGACTTCTGGGCCCAATTCTCCCTTTTACAAAGCCTTGATTGAATCTGGCCTTGGCACAGACT TTTCTCCTGATGTTGGATATAATGGCTACACGAGGGAGGCCTACTTTAGTGTCGGCCTCCAAGGGATTGT GGAGAAAGACATTGAGACCGTCAGAAGCCTCATAGACAGAACGATTGATGAAGTAGTTGAGAAAGGATTT GAAGATGATCGAATTGAGGCTTTACTTCATAAAATTGAAATACAGATGAAACATCAGTCTACCAGCTTTG GGCTGATGCTGACATCATACATAGCTTCTTGCTGGAACCATGATGGGGACCCTGTGGAGCTCTTGAAGTT GGGAAATCAGTTAGCTAAATTCAGACAGTGCCTGCAGGAAAATCCAAAATTTTTGCAAGAAAAAGTAAAA CAGTATTTTAAGAATAACCAGCATAAGCTGACTTTATCGATGAGGCCAGATGACAAGTATCACGAGAAGC AGGCACAGGTGGAAGCCACGAAGCTCAAGCAGAAGGTCGAGGCTCTGTCCCCCGGAGACAGGCAGCAGAT CTACGAGAAAGGTCTAGAATTACGGAGTCAACAAAGCAAACCTCAAGATGCCTCTTGTCTGCCAGCGTTG AAAGTTTCCGATATTGAACCCACCATACCTGTCACAGAGTTGGACGTGGTCCTGACAGCTGGAGATATCC CTGTTCAGTACTGCGCCCAGCCCACCAATGGCATGGTGTATTTCCGGGCCTTCTCCAGCCTGAACACACT CCCCGAGGAGCTGAGGCCCTATGTGCCCCTCTTCTGCAGCGTCCTCACCAAGCTGGGCTGCGGCCTTCTT GACTACCGGGAGCAGGCTCAGCAGATAGAATTGAAGACCGGAGGGATGAGTGCTTCTCCCCACGTGCTCC CCGACGACTCACACATGGACACCTACGAGCAGGGTGTGCTTTTCTCCTCTCTCTGCCTGGATCGAAACCT GCCAGACATGATGCAGCTATGGAGTGAAATATTTAACAACCCGTGCTTTGAAGAAGAGGAGCACTTCAAG GTGCTGGTGAAGATGACCGCCCAGGAGCTCGCCAATGGAATTCCTGACTCTGGGCACCTGTACGCATCCA TCAGGGCAGGCCGGACCCTCACGCCCGCAGGGGACCTGCAGGAGACCTTCAGCGGGATGGATCAGGTGCG GCTGATGAAGAGGATTGCAGAAATGACAGATATCAAACCCATCCTGAGGAAGCTCCCACGTATCAAGAAA CACTTGTTAAATGGTGATAATATGAGGTGTTCAGTGAATGCGACTCCTCAGCAGATGCCTCAGACAGAAA AAGCGGTCGAAGACTTCCTTAGAAGCATCGGTCGGAGTAAAAAGGAACGGAGGCCTGTGCGCCCACACAC GGTCGAGAAACCTGTGCCCAGCAGCTCTGGTGGAGATGCCCACGTTCCCCATGGCTCCCAGGTCATTAGG AAGCTGGTCATGGAACCCACCTTCAAGCCCTGGCAGATGAAGACTCACTTCCTGATGCCCTTCCCGGTGA ATTACGTGGGTGAATGCATCCGAACTGTCCCCTACACGGACCCAGATCATGCCAGTTTTGAAATCCTTGC ACGTTTGATGACTGCCAAATTCTTGCATACAGAAATTCGAGAAAAAGGCGGTGCTTATGGTGGAGGCGCA AAACTCAGCCACAATGGGATTTTCACCCTTTACTCTTACAGGGACCCAAATACAATAGAGACGCTCCAGT CTTTTGGGAAGGCTGTCGACTGGGCTAAGTCTGGAAAATTCACACAGCAAGACATCGACGAAGCCAAACT TTCTGTCTTCTCAACCGTAGATGCTCCTGTCGCTCCTTCAGACAAAGGAATGGACCACTTCTTGTACGGC CTCTCGGATGAGATGAAGCAGGCCCACAGAGAGCAGCTCTTTGCTGTCAGCCACGACAAGCTCCTGGCCG TGAGCGATAGGTACCTCGGCACTGGGAAAAGCACACACGGCCTGGCCATCCTCGGACCCGAGAACCCGAA AATTGCCAAGGACCCATCCTGGATCATCCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210596 representing NM_014889
Red=Cloning site Green=Tags(s) MWRCGGRQGLCVLRRLSGGHAHHRAWRWNSNRACERALQYKLGDKIHGFTVNQVTSVPELFLTAVKLTHD DTGARYLHLAREDTNNLFSVQFRTTPMDSTGVPHILEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAFTA SDYTVYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPSDPQTPLVFKGVVFNEMKGAF TDNERIFSQHLQNRLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFPLEQHLKQI HEEALSKFQKIEPSTVVPAQTPWDKPREFQITCGPDSFATDPSKQTTVSVSFLLPDITDTFEAFTLSLLS SLLTSGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIVEKDIETVRSLIDRTIDEVVEKGF EDDRIEALLHKIEIQMKHQSTSFGLMLTSYIASCWNHDGDPVELLKLGNQLAKFRQCLQENPKFLQEKVK QYFKNNQHKLTLSMRPDDKYHEKQAQVEATKLKQKVEALSPGDRQQIYEKGLELRSQQSKPQDASCLPAL KVSDIEPTIPVTELDVVLTAGDIPVQYCAQPTNGMVYFRAFSSLNTLPEELRPYVPLFCSVLTKLGCGLL DYREQAQQIELKTGGMSASPHVLPDDSHMDTYEQGVLFSSLCLDRNLPDMMQLWSEIFNNPCFEEEEHFK VLVKMTAQELANGIPDSGHLYASIRAGRTLTPAGDLQETFSGMDQVRLMKRIAEMTDIKPILRKLPRIKK HLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRPVRPHTVEKPVPSSSGGDAHVPHGSQVIR KLVMEPTFKPWQMKTHFLMPFPVNYVGECIRTVPYTDPDHASFEILARLMTAKFLHTEIREKGGAYGGGA KLSHNGIFTLYSYRDPNTIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDAPVAPSDKGMDHFLYG LSDEMKQAHREQLFAVSHDKLLAVSDRYLGTGKSTHGLAILGPENPKIAKDPSWIIR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014889 |
| ORF Size | 3111 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014889.2, NP_055704.2 |
| RefSeq Size | 3465 bp |
| RefSeq ORF | 3114 bp |
| Locus ID | 10531 |
| UniProt ID | Q5JRX3 |
| Domains | Peptidase_M16_C |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease. [provided by RefSeq, Dec 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210596 | PITRM1 (Myc-DDK-tagged)-Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 10,832.00 |
|
| RC210596L1 | Lenti ORF clone of Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 13,232.00 |
|
| RC210596L2 | Lenti ORF clone of Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 12,260.00 |
|
| RC210596L3 | Lenti ORF clone of Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 12,260.00 |
|
| RC210596L4 | Lenti ORF clone of Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 12,260.00 |
|
| SC114737 | PITRM1 (untagged)-Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 10,840.00 |
