TDP43 (TARDBP) (NM_007375) Human Tagged ORF Clone
CAT#: RG210639
- TrueORF®
TARDBP (tGFP-tagged) - Human TAR DNA binding protein (TARDBP)
ORF Plasmid: DDK
"NM_007375" in other vectors (7)
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CNY 5,256.00
Cited in 8 publications. |
CNY 1,999.00
CNY 3,600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ALS10; TDP-43 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210639 representing NM_007375
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAATATATTCGGGTAACCGAAGATGAGAACGATGAGCCCATTGAAATACCATCGGAAGACGATG GGACGGTGCTGCTCTCCACGGTTACAGCCCAGTTTCCAGGGGCGTGTGGGCTTCGCTACAGGAATCCAGT GTCTCAGTGTATGAGAGGTGTCCGGCTGGTAGAAGGAATTCTGCATGCCCCAGATGCTGGCTGGGGAAAT CTGGTGTATGTTGTCAACTATCCAAAAGATAACAAAAGAAAAATGGATGAGACAGATGCTTCATCAGCAG TGAAAGTGAAAAGAGCAGTCCAGAAAACATCCGATTTAATAGTGTTGGGTCTCCCATGGAAAACAACCGA ACAGGACCTGAAAGAGTATTTTAGTACCTTTGGAGAAGTTCTTATGGTGCAGGTCAAGAAAGATCTTAAG ACTGGTCATTCAAAGGGGTTTGGCTTTGTTCGTTTTACGGAATATGAAACACAAGTGAAAGTAATGTCAC AGCGACATATGATAGATGGACGATGGTGTGACTGCAAACTTCCTAATTCTAAGCAAAGCCAAGATGAGCC TTTGAGAAGCAGAAAAGTGTTTGTGGGGCGCTGTACAGAGGACATGACTGAGGATGAGCTGCGGGAGTTC TTCTCTCAGTACGGGGATGTGATGGATGTCTTCATCCCCAAGCCATTCAGGGCCTTTGCCTTTGTTACAT TTGCAGATGATCAGATTGCGCAGTCTCTTTGTGGAGAGGACTTGATCATTAAAGGAATCAGCGTTCATAT ATCCAATGCCGAACCTAAGCACAATAGCAATAGACAGTTAGAAAGAAGTGGAAGATTTGGTGGTAATCCA GGTGGCTTTGGGAATCAGGGTGGATTTGGTAATAGCAGAGGGGGTGGAGCTGGTTTGGGAAACAATCAAG GTAGTAATATGGGTGGTGGGATGAACTTTGGTGCGTTCAGCATTAATCCAGCCATGATGGCTGCCGCCCA GGCAGCACTACAGAGCAGTTGGGGTATGATGGGCATGTTAGCCAGCCAGCAGAACCAGTCAGGCCCATCG GGTAATAACCAAAACCAAGGCAACATGCAGAGGGAGCCAAACCAGGCCTTCGGTTCTGGAAATAACTCTT ATAGTGGCTCTAATTCTGGTGCAGCAATTGGTTGGGGATCAGCATCCAATGCAGGGTCGGGCAGTGGTTT TAATGGAGGCTTTGGCTCAAGCATGGATTCTAAGTCTTCTGGCTGGGGAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210639 representing NM_007375
Red=Cloning site Green=Tags(s) MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGN LVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLK TGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREF FSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNP GGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPS GNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007375 |
ORF Size | 1242 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007375.4 |
RefSeq Size | 4236 bp |
RefSeq ORF | 1245 bp |
Locus ID | 23435 |
UniProt ID | Q13148 |
Domains | RRM |
Protein Families | Transcription Factors |
Gene Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
Citations (8)
The use of this cDNA Clones has been cited in the following citations: |
---|
TDP-43 mutations link Amyotrophic Lateral Sclerosis with R-loop homeostasis and R loop-mediated DNA damage
,null,
PLoS Genetics
,PubMed ID 33301444
[TDP43]
|
The Role of HDAC6 in TDP-43-Induced Neurotoxicity and UPS Impairment
,null,
Frontiers in Cell and Developmental Biology
,PubMed ID 33282865
[TDP43]
|
The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response
,null,
Journal of Neuroinflammation
,PubMed ID 33054766
[TDP43]
|
An ALS-linked mutation in TDP-43 disrupts normal protein interactions in the motor neuron response to oxidative stress
,Feneberg, E;Gordon, D;Thompson, AG;Finelli, MJ;Dafinca, R;Candalija, A;Charles, PD;Mäger, I;Wood, MJ;Fischer, R;Kessler, BM;Gray, E;Turner, MR;Talbot, K;,
Neurobiol. Dis.
,PubMed ID 32800996
[TDP43]
|
PTK2/FAK regulates UPS impairment via SQSTM1/p62 phosphorylation in TARDBP/TDP-43 proteinopathies
,null,
Autophagy
,PubMed ID 31690171
[TDP43]
|
Clusterin protects neurons against intracellular proteotoxicity
,Gregory, JM;Whiten, DR;Brown, RA;Barros, TP;Kumita, JR;Yerbury, JJ;Satapathy, S;McDade, K;Smith, C;Luheshi, LM;Dobson, CM;Wilson, MR;,
Acta Neuropathol Commun
,PubMed ID 29115989
[TDP43]
|
Addition of exogenous SOD1 aggregates causes TDP-43 mislocalisation and aggregation
,Zeineddine, R;Farrawell, NE;Lambert-Smith, IA;Yerbury, JJ;,
Cell Stress Chaperones
,PubMed ID 28560609
[TDP43]
|
Distinct partitioning of ALS associated TDP-43, FUS and SOD1 mutants into cellular inclusions
,Farrawell, NE;Lambert-Smith, IA;Warraich, ST;Blair, IP;Saunders, DN;Hatters, DM;Yerbury, JJ;,
Sci Rep
,PubMed ID 26293199
[TDP43]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210639 | TARDBP (Myc-DDK-tagged)-Human TAR DNA binding protein (TARDBP) |
CNY 3,656.00 |
|
RC210639L1 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC210639L2 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), mGFP tagged |
CNY 6,056.00 |
|
RC210639L3 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC210639L4 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), mGFP tagged |
CNY 6,056.00 |
|
SC115570 | TARDBP (untagged)-Human TAR DNA binding protein (TARDBP) |
CNY 5,488.00 |
|
SC322765 | TARDBP (untagged)-Human TAR DNA binding protein (TARDBP) |
CNY 5,488.00 |