TMEM201 (NM_001010866) Human Tagged ORF Clone

CAT#: RG210685

  • TrueORF®

TMEM201 (tGFP-tagged) - Human transmembrane protein 201 (TMEM201), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001010866" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms Ima1; NET5; SAMP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG210685 representing NM_001010866
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGAGTGAGCGCGCTGCTGGCCCGCTGCCCCACGGCCGGCCTGGCCGGCGGCCTGGGGGTCACGG
CGTGCGCCGCGGCCGGCGTGTTGCTCTACCGGATCGCGCGGAGGATGAAGCCAACGCACACGATGGTCAA
CTGCTGGTTCTGCAACCAGGATACGCTGGTGCCCTATGGGAACCGCAACTGCTGGGACTGTCCCCACTGC
GAGCAGTACAACGGCTTCCAGGAGAACGGCGACTACAACAAGCCGATCCCCGCCCAGTACTTGGAGCACC
TGAACCACGTGGTGAGCAGCGCGCCCAGCCTGCGCGACCCTTCGCAGCCGCAGCAGTGGGTGAGCAGCCA
AGTCCTGCTGTGCAAGAGGTGCAACCACCACCAGACCACCAAGATCAAGCAGCTGGCCGCCTTCGCTCCC
CGCGAGGAGGGCAGGTATGACGAGGAGGTCGAGGTGTACCGGCATCACCTGGAGCAGATGTACAAGCTGT
GCCGGCCGTGCCAAGCGGCTGTGGAGTACTACATCAAGCACCAGAACCGCCAGCTGCGCGCCCTGTTGCT
CAGCCACCAGTTCAAGCGCCGGGAGGCCGACCAGACCCACGCACAGAACTTCTCCTCCGCCGTGAAGTCC
CCGGTCCAGGTCATCCTGCTCCGTGCCCTCGCCTTCCTGGCCTGCGCCTTCCTACTGACCACCGCGCTGT
ATGGGGCCAGCGGACACTTCGCCCCAGGCACCACTGTGCCCCTGGCCCTGCCACCTGGTGGCAATGGCTC
AGCCACACCTGACAATGGCACCACCCCTGGGGCCGAGGGCTGGCGGCAGTTGCTGGGCCTACTCCCCGAG
CACATGGCGGAGAAGCTGTGTGAGGCCTGGGCCTTTGGGCAGAGCCACCAGACGGGCGTCGTGGCACTGG
GCCTACTCACCTGCCTGCTGGCAATGCTGCTGGCTGGCCGCATCAGGCTCCGGAGGATCGATGCCTTCTG
CACCTGCCTGTGGGCCCTGCTGCTGGGGCTGCACCTGGCTGAGCAGCACCTGCAGGCCGCCTCGCCTAGC
TGGCTAGACACGCTCAAGTTCAGCACCACATCTTTGTGCTGCCTGGTTGGCTTCACGGCGGCTGTGGCCA
CAAGGAAGGCAACGGGCCCACGGAGGTTCCGGCCCCGAAGGTCAGAGAAGCAGCCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG210685 representing NM_001010866
Red=Cloning site Green=Tags(s)

MEGVSALLARCPTAGLAGGLGVTACAAAGVLLYRIARRMKPTHTMVNCWFCNQDTLVPYGNRNCWDCPHC
EQYNGFQENGDYNKPIPAQYLEHLNHVVSSAPSLRDPSQPQQWVSSQVLLCKRCNHHQTTKIKQLAAFAP
REEGRYDEEVEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFKRREADQTHAQNFSSAVKS
PVQVILLRALAFLACAFLLTTALYGASGHFAPGTTVPLALPPGGNGSATPDNGTTPGAEGWRQLLGLLPE
HMAEKLCEAWAFGQSHQTGVVALGLLTCLLAMLLAGRIRLRRIDAFCTCLWALLLGLHLAEQHLQAASPS
WLDTLKFSTTSLCCLVGFTAAVATRKATGPRRFRPRRSEKQP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001010866
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001010866.4
RefSeq Size 3846 bp
RefSeq ORF 1179 bp
Locus ID 199953
UniProt ID Q5SNT2
Protein Families Transmembrane
Gene Summary Involved in nuclear movement during fibroblast polarization and migration. Proposed to be involved in actin-dependent nuclear movement via association with transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow (By similarity). Overexpression can recruit Ran GTPase to the nuclear periphery (PubMed:27541860).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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