CD28 (NM_006139) Human Tagged ORF Clone
CAT#: RG211318
- TrueORF®
CD28 (tGFP-tagged) - Human CD28 molecule (CD28)
ORF Plasmid: DDK
"NM_006139" in other vectors (6)
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CNY 5,200.00
Cited in 2 publications. |
CNY 1,999.00
CNY 3,600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Tp44 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG211318 representing NM_006139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGCTGCTCTTGGCTCTCAACTTATTCCCTTCAATTCAAGTAACAGGAAACAAGATTTTGGTGA AGCAGTCGCCCATGCTTGTAGCGTACGACAATGCGGTCAACCTTAGCTGCAAGTATTCCTACAATCTCTT CTCAAGGGAGTTCCGGGCATCCCTTCACAAAGGACTGGATAGTGCTGTGGAAGTCTGTGTTGTATATGGG AATTACTCCCAGCAGCTTCAGGTTTACTCAAAAACGGGGTTCAACTGTGATGGGAAATTGGGCAATGAAT CAGTGACATTCTACCTCCAGAATTTGTATGTTAACCAAACAGATATTTACTTCTGCAAAATTGAAGTTAT GTATCCTCCTCCTTACCTAGACAATGAGAAGAGCAATGGAACCATTATCCATGTGAAAGGGAAACACCTT TGTCCAAGTCCCCTATTTCCCGGACCTTCTAAGCCCTTTTGGGTGCTGGTGGTGGTTGGTGGAGTCCTGG CTTGCTATAGCTTGCTAGTAACAGTGGCCTTTATTATTTTCTGGGTGAGGAGTAAGAGGAGCAGGCTCCT GCACAGTGACTACATGAACATGACTCCCCGCCGCCCCGGGCCCACCCGCAAGCATTACCAGCCCTATGCC CCACCACGCGACTTCGCAGCCTATCGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211318 representing NM_006139
Red=Cloning site Green=Tags(s) MLRLLLALNLFPSIQVTGNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYG NYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHL CPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYA PPRDFAAYRS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006139 |
ORF Size | 660 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006139.4 |
RefSeq Size | 3804 bp |
RefSeq ORF | 663 bp |
Locus ID | 940 |
UniProt ID | P10747 |
Domains | IGv |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, T cell receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis |
Gene Summary | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
A Quantitative Assessment of Costimulation and Phosphatase Activity on Microclusters in Early T Cell Signaling
,Witsenburg, JJ;Glauner, H;M?ller, JP;,
PLOS One October 30, 2013
[CD28]
|
A Quantitative Assessment of Costimulation and Phosphatase Activity on Microclusters in Early T Cell Signaling
,null,
PLoS ONE
,PubMed ID 24205378
[CD28]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211318 | CD28 (Myc-DDK-tagged)-Human CD28 molecule (CD28) |
CNY 3,600.00 |
|
RC211318L1 | Lenti ORF clone of Human CD28 molecule (CD28), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC211318L2 | Lenti ORF clone of Human CD28 molecule (CD28), mGFP tagged |
CNY 5,890.00 |
|
RC211318L3 | Lenti ORF clone of Human CD28 molecule (CD28), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC211318L4 | Lenti ORF clone of Human CD28 molecule (CD28), mGFP tagged |
CNY 6,000.00 |
|
SC116267 | CD28 (untagged)-Human CD28 molecule (CD28) |
CNY 3,600.00 |