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SLC5A4 (NM_014227) Human Tagged ORF Clone
CAT＃: RG211428
- TrueORF®
SLC5A4 (tGFP-tagged) - Human solute carrier family 5 (low affinity glucose cotransporter), member 4 (SLC5A4)
ORF Plasmid: DDK
"NM_014227" in other vectors (4)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DJ90G24.4; SAAT1; SGLT3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211428 representing NM_014227
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTACGGTTAGCCCCAGCACCATAGCTGAGACCCCAGAGCCACCTCCATTGTCTGACCACATCC GAAATGCTGCTGACATCTCAGTCATTGTCATCTATTTTCTGGTGGTGATGGCTGTTGGGCTGTGGGCGAT GCTGAAGACCAACCGAGGTACTATAGGAGGCTTCTTCCTCGCTGGTCGTGATATGGCCTGGTGGCCGATG GGCGCCTCTCTCTTTGCCAGTAACATCGGCAGCAACCACTATGTGGGGCTGGCTGGGACAGGAGCAGCTT CAGGAGTCGCCACCGTAACATTTGAATGGACTTCCTCAGTAATGTTGCTGATTCTTGGGTGGATCTTTGT CCCTATCTACATCAAGTCGGGGGTGATGACCATGCCGGAATATCTCAAGAAGCGGTTTGGTGGGGAGCGA CTCCAGGTCTACCTCTCCATCCTCTCCCTCTTCATCTGTGTGGTTTTGTTAATTTCTGCAGACATATTTG CTGGAGCCATATTCATCAAGCTGGCCTTGGGATTGGACCTTTACCTGGCAATCTTCATCCTCTTGGCTAT GACTGCTGTTTACACCACCACTGGGGGCTTGGCCTCGGTGATTTACACAGACACCCTCCAGACCATCATC ATGCTGATTGGCTCTTTTATTCTCATGGGGTTTGCATTTAACGAAGTTGGAGGTTATGAGAGCTTTACCG AGAAGTACGTGAATGCCACCCCATCCGTAGTCGAGGGGGACAACTTGACAATCAGTGCCAGTTGCTACAC ACCTCGGGCGGACTCCTTCCATATCTTCCGAGATGCTGTGACTGGGGACATTCCATGGCCAGGAATTATA TTTGGAATGCCCATTACAGCTTTGTGGTACTGGTGCACAAATCAGGTCATTGTGCAGCGCTGCCTGTGTG GCAAGGACATGTCTCACGTGAAGGCCGCTTGCATTATGTGTGCTTACCTGAAGCTGCTGCCCATGTTCCT CATGGTGATGCCGGGGATGATCAGCCGCATCCTGTACACAGATATGGTAGCATGTGTGGTACCTTCTGAA TGCGTGAAACACTGTGGCGTTGATGTTGGCTGCACCAACTACGCATACCCCACGATGGTGCTGGAACTGA TGCCCCAAGGACTGCGAGGCCTGATGCTTTCGGTCATGCTGGCCTCTCTCATGAGCTCCCTGACCTCCAT CTTCAACAGCGCCAGCACCCTCTTCACCATTGACCTCTACACCAAGATGCGGAAGCAAGCGTCGGAGAAA GAGCTCCTGATAGCTGGACGGATATTTGTTCTTCTATTAACTGTTGTGAGCATTGTGTGGGTCCCACTGG TACAAGTTTCTCAAAATGGACAACTAATCCATTACACAGAATCAATTTCTAGCTACCTTGGGCCTCCAAT TGCAGCTGTCTTTGTGCTTGCCATCTTCTGTAAAAGAGTCAATGAACAGGGAGCATTCTGGGGTCTAATG GTTGGACTTGCAATGGGCCTCATTCGTATGATAACAGAGTTTGCTTATGGAACAGGGAGTTGCTTGGCTC CCAGTAACTGTCCCAAGATTATCTGTGGAGTGCACTATCTGTACTTTTCCATCGTTCTCTTTTTTGGGTC CATGCTGGTCACCCTGGGAATTTCCCTCTTAACAAAACCCATTCCTGATGTACATCTGTACCGCCTGTGC TGGGTTCTTCGGAACAGTACAGAGGAGCGAATCGATATAGATGCAGAAGAGAAAAGTCAGGAAGAAACAG ATGATGGTGTTGAAGAAGATTATCCTGAGAAATCACGTGGATGCCTCAAGAAAGCTTATGACTTGTTCTG CGGTTTGCAGAAGGGACCCAAGCTAACCAAGGAGGAGGAGGAAGCCTTGAGCAAGAAGCTCACAGACACG TCTGAGAGGCCCTCGTGGAGGACAATAGTGAACATCAACGCCATCCTCCTCCTGGCTGTGGTGGTCTTTA TTCACGGCTACTATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211428 representing NM_014227
Red=Cloning site Green=Tags(s) MASTVSPSTIAETPEPPPLSDHIRNAADISVIVIYFLVVMAVGLWAMLKTNRGTIGGFFLAGRDMAWWPM GASLFASNIGSNHYVGLAGTGAASGVATVTFEWTSSVMLLILGWIFVPIYIKSGVMTMPEYLKKRFGGER LQVYLSILSLFICVVLLISADIFAGAIFIKLALGLDLYLAIFILLAMTAVYTTTGGLASVIYTDTLQTII MLIGSFILMGFAFNEVGGYESFTEKYVNATPSVVEGDNLTISASCYTPRADSFHIFRDAVTGDIPWPGII FGMPITALWYWCTNQVIVQRCLCGKDMSHVKAACIMCAYLKLLPMFLMVMPGMISRILYTDMVACVVPSE CVKHCGVDVGCTNYAYPTMVLELMPQGLRGLMLSVMLASLMSSLTSIFNSASTLFTIDLYTKMRKQASEK ELLIAGRIFVLLLTVVSIVWVPLVQVSQNGQLIHYTESISSYLGPPIAAVFVLAIFCKRVNEQGAFWGLM VGLAMGLIRMITEFAYGTGSCLAPSNCPKIICGVHYLYFSIVLFFGSMLVTLGISLLTKPIPDVHLYRLC WVLRNSTEERIDIDAEEKSQEETDDGVEEDYPEKSRGCLKKAYDLFCGLQKGPKLTKEEEEALSKKLTDT SERPSWRTIVNINAILLLAVVVFIHGYYA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014227 |
| ORF Size | 1977 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014227.1, NP_055042.1 |
| RefSeq Size | 2043 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 6527 |
| UniProt ID | Q9NY91 |
| Protein Families | Transmembrane |
| Gene Summary | Has electrogenic activity in response to glucose, and may function as a glucose sensor (PubMed:13130073, PubMed:17110502, PubMed:20421923, PubMed:22766068). Mediates influx of sodium ions into the cell but does not transport sugars (PubMed:13130073, PubMed:22766068). Also potently activated by imino sugars such as deoxynojirimycin (DNJ) (PubMed:17110502, PubMed:20421923, PubMed:22766068).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211428 | SLC5A4 (Myc-DDK-tagged)-Human solute carrier family 5 (low affinity glucose cotransporter), member 4 (SLC5A4) |
CNY 4,904.00 |
|
| RC211428L3 | Lenti ORF clone of Human solute carrier family 5 (low affinity glucose cotransporter), member 4 (SLC5A4), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC211428L4 | Lenti ORF clone of Human solute carrier family 5 (low affinity glucose cotransporter), member 4 (SLC5A4), mGFP tagged |
CNY 7,600.00 |
|
| SC304066 | SLC5A4 (untagged)-Human solute carrier family 5 (low affinity glucose cotransporter), member 4 (SLC5A4) |
CNY 10,550.00 |
