OSBPL6 (NM_032523) Human Tagged ORF Clone

CAT#: RG213081

  • TrueORF®

OSBPL6 (tGFP-tagged) - Human oxysterol binding protein-like 6 (OSBPL6), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_032523" in other vectors (6)

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CNY 8,440.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal antibody to OSBPL6 (oxysterol binding protein-like 6)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms ORP6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG213081 representing NM_032523
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTCAGATGAGAAGGGCATTTCCCCTGCTCATAAAACATCCACTCCAACCCATAGAAGTGCCTCCT
CTTCAACATCCTCCCAAAGGGACAGTAGGCAGAGTATTCACATACTGGAGAGGACTGCTTCCTCTAGCAC
CGAGCCCTCTGTAAGTCGGCAATTGCTAGAACCGGAGCCAGTCCCCCTCTCCAAGGAAGCTGACAGCTGG
GAAATTATAGAAGGGCTGAAAATAGGCCAAACCAATGTCCAGAAACCAGACAAACATGAGGGCTTTATGC
TGAAGAAAAGAAAATGGCCTTTAAAAGGCTGGCACAAGCGTTTTTTTGTCCTGGATAATGGAATGTTAAA
GTATTCAAAGGCACCACTCGATATTCAGAAAGGAAAGGTCCATGGGAGCATAGATGTGGGACTCTCAGTC
ATGTCAATTAAAAAGAAAGCTCGAAGAATAGACCTTGACACCGAAGAGCACATCTATCATTTGAAGGTGA
AATCCCAGGACTGGTTTGATGCATGGGTCTCCAAACTGCGACATCATCGGTTGTATCGTCAGAATGAAAT
TGTGAGATCACCAAGAGATGCTAGTTTTCACATATTTCCTTCAACGTCCACAGCTGAATCCTCACCAGCT
GCTAATGTTTCTGTAATGGATGGAAAGATGCAACCAAACAGCTTTCCGTGGCAGTCCCCTTTACCATGCA
GCAATAGCCTCCCTGCAACGTGCACAACTGGCCAGAGTAAAGTGGCAGCCTGGTTACAGGACTCGGAAGA
GATGGACAGGTGTGCAGAAGATCTTGCACATTGCCAGTCAAACCTTGTGGAACTTAGCAAACTCCTGCAA
AATTTGGAAATACTTCAGAGAACTCAGTCAGCACCTAACTTTACTGACATGCAGGCTAACTGTGTAGATA
TTTCAAAGAAAGACAAGCGGGTCACAAGACGATGGAGAACAAAAAGTGTCAGCAAAGATACAAAAATACA
ACTGCAGGTTCCTTTCAGTGCTACCATGTCACCAGTTCGCTTGCATTCCTCCAACCCCAACCTTTGTGCA
GATATTGAATTTCAGACTCCCCCTAGCCACCTCACTGACCCTCTGGAAAGTTCAACAGATTATACAAAGC
TGCAAGAAGAATTTTGTCTAATCGCACAGAAAGTGCATTCTCTTTTGAAGTCTGCATTTAATAGCATAGC
TATAGAGAAGGAGAAGCTGAAGCAGATGGTTTCCGAGCAGGATCACAGTAAAGGCCACAGCACGCAGATG
GCACGGCTCCGACAGTCACTGTCTCAGGCACTCAACCAGAATGCTGAACTAAGGAGTCGGTTGAACAGAA
TACATTCAGAGTCTATTATTTGTGATCAGGTTGTCAGTGTAAATATTATTCCTAGCCCTGATGAGGCTGG
TGAGCAAATCCATGTCAGTCTCCCCTTATCACAGCAAGTAGCCAATGAGAGCCGCCTCTCCATGTCAGAG
TCTGTTTCTGAGTTCTTTGATGCCCAAGAGGTGCTCCTCTCTGCAAGTTCGTCAGAGAATGAGGCTTCAG
ATGATGAGTCTTACATCAGTGATGTGAGTGATAATATATCTGAAGACAACACCAGTGTTGCAGACAATAT
TTCTCGGCAAATCCTGAATGGGGAGCTTACAGGAGGGGCCTTCCGAAATGGGCGTCGAGCATGCCTGCCA
GCTCCTTGTCCTGACACCAGTAACATTAACCTGTGGAATATCTTGAGGAACAACATTGGTAAAGACCTGT
CTAAAGTCTCTATGCCTGTGGAGCTAAACGAGCCGCTCAACACCCTGCAGCACCTCTGTGAGGAAATGGA
ATACAGCGAGCTCCTGGACAAGGCTTCGGAAACTGATGATCCATATGAGCGCATGGTTCTCGTTGCCGCA
TTTGCAGTTTCAGGATACTGCTCCACCTATTTCAGAGCAGGAAGTAAGCCATTCAACCCAGTCCTTGGGG
AGACTTATGAATGCATTAGAGAAGACAAGGGATTCCGCTTTTTCTCAGAACAGGTTAGCCATCATCCACC
CATTTCTGCCTGTCACTGTGAATCAAAGAATTTTGTGTTTTGGCAAGATATCAGATGGAAAAACAAGTTC
TGGGGGAAGTCGATGGAAATCCTGCCTGTTGGAACACTGAATGTCATGCTTCCAAAGTATGGAGATTACT
ATGTGTGGAATAAAGTCACCACTTGCATACACAACATCCTCAGTGGGAGAAGATGGATAGAACATTATGG
AGAAGTAACCATCAGAAATACCAAAAGCAGTGTTTGCATTTGCAAACTCACATTTGTCAAGGTGAATTAT
TGGAATTCTAACATGAATGAAGTCCAGGGGGTGGTGATAGATCAGGAGGGGAAGGCGGTGTACCGGCTGT
TTGGAAAGTGGCATGAAGGACTCTACTGTGGTGTGGCCCCCTCTGCAAAGTGCATTTGGAGACCAGGTTC
CATGCCAACAAACTATGAGCTGTACTATGGCTTCACAAGGTTTGCTATTGAGCTCAATGAGTTAGATCCA
GTACTAAAAGATCTCCTTCCACCAACAGACGCCCGGTTCCGGCCAGATCAAAGATTTTTGGAAGAAGGAA
ATTTAGAAGCTGCAGCATCAGAGAAGCAAAGAGTAGAGGAACTCCAGAGATCTCGGAGACGATATATGGA
AGAAAACAATCTTGAACATATACCAAAATTTTTTAAAAAAGTTATTGATGCCAATCAAAGAGAAGCCTGG
GTTTCTAACGACACCTACTGGGAGCTTCGAAAGGACCCTGGGTTTAGCAAAGTAGACAGCCCTGTTCTTT
GG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG213081 representing NM_032523
Red=Cloning site Green=Tags(s)

MSSDEKGISPAHKTSTPTHRSASSSTSSQRDSRQSIHILERTASSSTEPSVSRQLLEPEPVPLSKEADSW
EIIEGLKIGQTNVQKPDKHEGFMLKKRKWPLKGWHKRFFVLDNGMLKYSKAPLDIQKGKVHGSIDVGLSV
MSIKKKARRIDLDTEEHIYHLKVKSQDWFDAWVSKLRHHRLYRQNEIVRSPRDASFHIFPSTSTAESSPA
ANVSVMDGKMQPNSFPWQSPLPCSNSLPATCTTGQSKVAAWLQDSEEMDRCAEDLAHCQSNLVELSKLLQ
NLEILQRTQSAPNFTDMQANCVDISKKDKRVTRRWRTKSVSKDTKIQLQVPFSATMSPVRLHSSNPNLCA
DIEFQTPPSHLTDPLESSTDYTKLQEEFCLIAQKVHSLLKSAFNSIAIEKEKLKQMVSEQDHSKGHSTQM
ARLRQSLSQALNQNAELRSRLNRIHSESIICDQVVSVNIIPSPDEAGEQIHVSLPLSQQVANESRLSMSE
SVSEFFDAQEVLLSASSSENEASDDESYISDVSDNISEDNTSVADNISRQILNGELTGGAFRNGRRACLP
APCPDTSNINLWNILRNNIGKDLSKVSMPVELNEPLNTLQHLCEEMEYSELLDKASETDDPYERMVLVAA
FAVSGYCSTYFRAGSKPFNPVLGETYECIREDKGFRFFSEQVSHHPPISACHCESKNFVFWQDIRWKNKF
WGKSMEILPVGTLNVMLPKYGDYYVWNKVTTCIHNILSGRRWIEHYGEVTIRNTKSSVCICKLTFVKVNY
WNSNMNEVQGVVIDQEGKAVYRLFGKWHEGLYCGVAPSAKCIWRPGSMPTNYELYYGFTRFAIELNELDP
VLKDLLPPTDARFRPDQRFLEEGNLEAAASEKQRVEELQRSRRRYMEENNLEHIPKFFKKVIDANQREAW
VSNDTYWELRKDPGFSKVDSPVLW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032523
ORF Size 2802 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032523.2, NP_115912.1
RefSeq Size 3794 bp
RefSeq ORF 2805 bp
Locus ID 114880
UniProt ID Q9BZF3
Domains Oxysterol_BP, PH
Gene Summary This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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