ATP2B1 (NM_001001323) Human Tagged ORF Clone
CAT#: RG213177
- TrueORF®
ATP2B1 (tGFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1.
ORF Plasmid: DDK
"NM_001001323" in other vectors (4)
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CNY 12,830.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PMCA1; PMCA1kb |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213177 representing NM_001001323.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTGAAGGAAGCTAATCAT GATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTA CGAAAAATACAGGAAAGCTATGGAGATGTCTATGGAATTTGCACCAAATTGAAAACATCTCCCAATGAA GGTTTAAGTGGAAACCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCT AAAAAGCCAAAAACCTTTCTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAA ATTGCAGCCATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGA GAAGTTTCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCAATCCTCTTG TCTGTAGTGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAAAAACAGTTTAGAGGTTTG CAGAGCCGAATTGAACAAGAACAGAAGTTCACTGTCATCAGGGGTGGTCAGGTCATTCAGATACCTGTA GCTGACATTACTGTTGGAGATATTGCTCAAGTGAAATATGGTGATCTTCTTCCAGCTGACGGCATACTT ATTCAAGGCAACGATCTTAAAATTGATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCT TTAGATAAGGATCCCTTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACA GCTGTAGGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAAGAG AAGAAAGATGAGAAGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAGAATCGCAACAAA GCAAAAGCCCAGGATGGTGCAGCCATGGAAATGCAGCCATTGAAGAGTGAAGAAGGTGGAGATGGTGAT GAAAAAGATAAAAAGAAAGCAAATTTGCCAAAAAAGGAAAAATCTGTTTTACAAGGGAAACTTACAAAA CTGGCTGTTCAGATTGGCAAAGCAGGTCTGTTGATGTCTGCCATCACAGTTATCATTCTAGTATTATAT TTTGTCATTGACACCTTCTGGGTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAA TACTTTGTGAAGTTCTTCATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTT GCAGTCACGATCTCACTGGCTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGGCATCTG GATGCTTGTGAAACCATGGGAAATGCTACAGCTATTTGTTCAGATAAAACAGGAACTTTGACAATGAAC AGAATGACAGTCGTTCAAGCTTACATAAATGAAAAACATTATAAAAAGGTTCCTGAACCAGAAGCTATT CCACCAAATATTTTGTCCTATCTTGTAACAGGAATTTCTGTGAATTGTGCTTATACATCAAAAATATTG CCACCAGAGAAAGAGGGTGGATTACCTCGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTT CTTTTGGATTTAAAACGGGATTATCAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTC TACACCTTCAATTCTGTTAGGAAGTCCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATA TTCAGCAAGGGTGCATCTGAGATAATTCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAATGGTGAGGCA AAAGTATTCAGACCAAGGGACCGTGATGATATTGTAAAAACTGTGATTGAACCGATGGCATCAGAAGGC TTGAGAACCATATGTCTTGCATTCAGAGATTTTCCAGCAGGAGAACCAGAACCAGAGTGGGATAATGAA AATGATATTGTCACCGGCCTTACATGCATTGCTGTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTG CCAGATGCAATTAAAAAGTGTCAGAGGGCTGGAATTACTGTGCGGATGGTCACTGGTGATAATATTAAT ACTGCTCGGGCCATTGCTACCAAATGTGGTATTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGT AAAGATTTTAACAGAAGAATACGAAATGAAAAAGGAGAGATTGAGCAAGAGAGGATAGACAAGATTTGG CCAAAACTTCGAGTACTTGCAAGATCATCTCCTACTGATAAGCATACACTGGTTAAAGGTATAATTGAC AGCACTGTCTCAGACCAACGCCAGGTTGTAGCTGTAACTGGTGATGGTACAAATGATGGCCCAGCACTA AAGAAAGCAGATGTTGGATTTGCAATGGGTATTGCTGGAACTGATGTAGCTAAAGAAGCATCCGATATT ATTCTCACAGATGACAACTTTACAAGCATTGTTAAAGCAGTTATGTGGGGACGAAATGTCTATGACAGC ATCTCAAAATTCCTTCAGTTCCAACTTACTGTTAATGTAGTAGCAGTGATTGTTGCTTTTACGGGCGCC TGCATTACTCAAGACTCACCGCTTAAGGCTGTGCAGATGCTGTGGGTAAACCTCATAATGGATACACTC GCTTCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCTCTCTTGCTTCGGAAACCTTATGGTAGAAAT AAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGGGTCATGCATTCTATCAACTTGTAGTAGTC TTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGATAGTGGAAGAAATGCTCCTTTGCATGCT CCTCCTTCAGAACATTATACTATTGTTTTTAATACCTTTGTGCTGATGCAACTTTTCAACGAAATAAAT GCCCGGAAAATTCATGGTGAAAGAAATGTATTCGAAGGAATCTTTAACAATGCCATCTTCTGCACAATT GTTTTAGGCACTTTTGTGGTACAGATAATAATTGTGCAGTTTGGTGGAAAACCTTTCAGTTGTTCAGAA CTTTCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAACATTACTCTGGGGCCAGCTTATT TCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTCATGGAACACAAAAGGAAGAAATA CCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGAAAGGGAGTTGCGGCGTGGCCAA ATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATGGATGTAGTGAATGCTTTCCAGAGTGGA AGTTCCATTCAGGGGGCTCTAAGGCGGCAACCCTCCATCGCCAGCCAGCATCATGATGTAACAAATATT TCTACCCCTACACATGTAGTGTTTTCCTCTTCTACTGCTTCTACTACTGTGGGGTATTCGAGTGGTGAA TGCATTTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG213177
Blue=ORF Red=Cloning site Green=Tag(s) MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNE GLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCG EVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPV ADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVT AVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGD EKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQ YFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMN RMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGL LLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEA KVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEV PDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIW PKLRVLARSSPTDKHTLVKGIIDSTVSDQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDI ILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTL ASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHA PPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSE LSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQ ILWFRGLNRIQTQMDVVNAFQSGSSIQGALRRQPSIASQHHDVTNISTPTHVVFSSSTASTTVGYSSGE CIS TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001001323 |
ORF Size | 3528 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001001323.2 |
RefSeq Size | 7121 bp |
RefSeq ORF | 3531 bp |
Locus ID | 490 |
UniProt ID | P20020 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Calcium signaling pathway |
MW | 129.5 kDa |
Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213177 | ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1. |
CNY 11,690.00 |
|
RC213177L3 | Lenti-ORF clone of ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1. |
CNY 13,590.00 |
|
RC213177L4 | Lenti-ORF clone of ATP2B1 (mGFP-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1. |
CNY 13,590.00 |
|
SC300162 | ATP2B1 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1 |
CNY 22,610.00 |