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COBRA1 (NELFB) (NM_015456) Human Tagged ORF Clone
CAT＃: RG213518
- TrueORF®
NELFB (tGFP-tagged) - Human cofactor of BRCA1 (COBRA1)
ORF Plasmid: DDK
"NM_015456" in other vectors (7)
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CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | COBRA1; NELF-B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213518 representing NM_015456
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGCGGGGCTGCAGGACCTGGGCGTGGCCAACGGCGAGGACCTGAAGGAGACCCTGACCAACTGCA CGGAGCCGCTCAAGGCCATCGAGCAGTTCCAGACAGAGAATGGTGTGCTGCTGCCATCTCTTCAGTCAGC CCTCCCCTTCTTGGACCTGCACGGGACGCCGCGGCTGGAGTTCCACCAGTCGGTATTCGATGAGCTGCGG GACAAGCTGCTGGAGCGAGTGTCAGCCATCGCTTCGGAGGGGAAGGCTGAGGAAAGGTACAAGAAGCTGG AAGACCTTCTGGAGAAGAGCTTTTCTCTGGTGAAGATGCCGTCCCTGCAGCCCGTGGTGATGTGCGTCAT GAAGCACCTGCCCAAGGTTCCGGAGAAAAAACTGAAGCTGGTTATGGCTGACAAGGAGCTGTATCGAGCC TGCGCCGTGGAGGTGAAGCGGCAGATCTGGCAAGACAACCAGGCCCTCTTCGGGGACGAGGTTTCCCCAC TCCTGAAGCAGTACATCCTGGAGAAGGAGAGCGCTCTCTTCAGTACAGAGCTCTCTGTCCTGCACAACTT TTTCAGTCCTTCCCCCAAGACCAGGCGCCAGGGCGAGGTGGTGCAGCGGCTGACGCGGATGGTGGGGAAG AACGTGAAGCTGTACGACATGGTGCTGCAGTTTCTGCGCACGCTCTTCCTGCGCACGCGGAATGTGCACT ACTGCACGCTGCGGGCTGAGCTGCTCATGTCCCTGCACGACCTGGACGTGGGTGAAATCTGCACCGTGGA CCCGTGCCACAAGTTCACCTGGTGCCTGGACGCCTGCATCCGAGAGCGGTTCGTGGACAGCAAGAGGGCG CGGGAGCTGCAGGGGTTTCTCGATGGCGTCAAGAAGGGCCAGGAGCAGGTGCTGGGGGACCTGTCCATGA TCCTGTGTGACCCCTTCGCCATCAACACGCTGGCACTGAGCACAGTCAGGCACCTGCAGGAGCTGGTCGG CCAGGAGACACTGCCCAGGGACAGCCCCGACCTCCTGCTGCTGCTCCGGCTGCTGGCGCTGGGCCAGGGA GCCTGGGACATGATCGACAGCCAGGTCTTCAAGGAGCCCAAGATGGAGGTAGAGCTCATCACCAGGTTCC TCCCGATGCTCATGTCCTTCCTGGTGGATGACTACACTTTCAATGTGGATCAGAAACTTCCGGCTGAGGA GAAAGCCCCAGTCTCATATCCAAACACACTTCCCGAAAGCTTCACTAAGTTTCTGCAGGAGCAGCGCATG GCCTGCGAGGTGGGGCTGTACTACGTCCTGCACATCACCAAGCAGAGGAACAAGAACGCGCTCCTCCGCC TGCTGCCCGGGCTGGTGGAGACCTTTGGCGACTTGGCCTTTGGCGACATCTTCCTCCACCTGCTCACGGG CAACCTTGCGCTGCTGGCCGACGAATTTGCCCTTGAGGACTTCTGCAGCAGCCTCTTCGATGGCTTCTTC CTCACCGCCTCTCCAAGGAAGGAGAACGTGCACCGGCACGCGCTGCGGCTCCTCATTCACCTGCACCCCA GGGTGGCCCCGTCTAAGCTGGAGGCGTTGCAGAAGGCCCTGGAGCCTACAGGCCAGAGCGGAGAGGCAGT GAAGGAGCTTTACTCCCAGCTCGGCGAGAAGCTGGAACAGCTGGATCACCGGAAGCCCAGCCCGGCACAG GCTGCGGAGACGCCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCCGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213518 representing NM_015456
Red=Cloning site Green=Tags(s) MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELR DKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRA CAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGK NVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRA RELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQG AWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRM ACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFF LTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQ AAETPALELPLPSVPAPAPL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015456 |
| ORF Size | 1740 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015456.3, NP_056271.2 |
| RefSeq Size | 2622 bp |
| RefSeq ORF | 1887 bp |
| Locus ID | 25920 |
| UniProt ID | Q8WX92 |
| Protein Families | Transcription Factors |
| Gene Summary | NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213518 | NELFB (Myc-DDK-tagged)-Human cofactor of BRCA1 (COBRA1) |
CNY 4,320.00 |
|
| RC213518L1 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), Myc-DDK-tagged |
CNY 6,720.00 |
|
| RC213518L2 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), mGFP tagged |
CNY 7,030.00 |
|
| RC213518L3 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), Myc-DDK-tagged |
CNY 7,030.00 |
|
| RC213518L4 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), mGFP tagged |
CNY 7,030.00 |
|
| SC114647 | NELFB (untagged)-Human cofactor of BRCA1 (COBRA1) |
CNY 6,400.00 |
|
| SC317995 | NELFB (untagged)-Human cofactor of BRCA1 (COBRA1) |
CNY 9,310.00 |
