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COBRA1 (NELFB) (NM_015456) Human Untagged Clone
CAT＃: SC317995
NELFB (untagged)-Human cofactor of BRCA1 (COBRA1)
CNY 9,310.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COBRA1; NELF-B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_015456, the custom clone sequence may differ by one or more nucleotides
CTGGCCGAGCTGGAGGGCGCCGGGGAGCGGGGCTCGGGCGGTCCCCGAGGCCCGGCGGAGCGGGCTTCTG GGGTGTCTGCGGCGGCGCCGGGGGAACGGGCTGGGGATGGGGCGCCTAGCCGGGCGGTGGCCGGGGCCTC GGCCATGTTCGCGGGGCTGCAGGACCTGGGCGTGGCCAACGGCGAGGACCTGAAGGAGACCCTGACCAAC TGCACGGAGCCGCTCAAGGCCATCGAGCAGTTCCAGACAGAGAATGGTGTGCTGCTGCCATCTCTTCAGT CAGCCCTCCCCTTCTTGGACCTGCACGGGACGCCGCGGCTGGAGTTCCACCAGTCGGTATTCGATGAGCT GCGGGACAAGCTGCTGGAGCGAGTGTCAGCCATCGCTTCGGAGGGGAAGGCTGAGGAAAGGTACAAGAAG CTGGAAGACCTTCTGGAGAAGAGCTTTTCTCTGGTGAAGATGCCGTCCCTGCAGCCCGTGGTGATGTGCG TCATGAAGCACCTGCCCAAGGTTCCGGAGAAAAAACTGAAGCTGGTTATGGCTGACAAGGAGCTGTATCG AGCCTGCGCCGTGGAGGTGAAGCGGCAGATCTGGCAAGACAACCAGGCCCTCTTCGGGGACGAGGTTTCC CCACTCCTGAAGCAGTACATCCTGGAGAAGGAGAGCGCTCTCTTCAGTACAGAGCTCTCTGTCCTGCACA ACTTTTTCAGTCCTTCCCCCAAGACCAGGCGCCAGGGCGAGGTGGTGCAGCGGCTGACGCGGATGGTGGG GAAGAACGTGAAGCTGTACGACATGGTGCTGCAGTTTCTGCGCACGCTCTTCCTGCGCACGCGGAATGTG CACTACTGCACGCTGCGGGCTGAGCTGCTCATGTCCCTGCACGACCTGGACGTGGGTGAAATCTGCACCG TGGACCCGTGCCACAAGTTCACCTGGTGCCTGGACGCCTGCATCCGAGAGCGGTTCGTGGACAGCAAGAG GGCGCGGGAGCTGCAGGGGTTTCTCGATGGCGTCAAGAAGGGCCAGGAGCAGGTGCTGGGGGACCTGTCC ATGATCCTGTGTGACCCCTTCGCCATCAACACGCTGGCACTGAGCACAGTCAGGCACCTGCAGGAGCTGG TCGGCCAGGAGACACTGCCCAGGGACAGCCCCGACCTCCTGCTGCTGCTCCGGCTGCTGGCGCTGGGCCA GGGAGCCTGGGACATGATCGACAGCCAGGTCTTCAAGGAGCCCAAGATGGAGGTAGAGCTCATCACCAGG TTCCTCCCGATGCTCATGTCCTTCCTGGTGGATGACTACACTTTCAATGTGGATCAGAAACTTCCGGCTG AGGAGAAAGCCCCAGTCTCATATCCAAACACACTTCCCGAAAGCTTCACTAAGTTTCTGCAGGAGCAGCG CATGGCCTGCGAGGTGGGGCTGTACTACGTCCTGCACATCACCAAGCAGAGGAACAAGAACGCGCTCCTC CGCCTGCTGCCCGGGCTGGTGGAGACCTTTGGCGACTTGGCCTTTGGCGACATCTTCCTCCACCTGCTCA CGGGCAACCTTGCGCTGCTGGCCGACGAATTTGCCCTTGAGGACTTCTGCAGCAGCCTCTTCGATGGCTT CTTCCTCACCGCCTCTCCAAGGAAGGAGAACGTGCACCGGCACGCGCTGCGGCTCCTCATTCACCTGCAC CCCAGGGTGGCCCCGTCTAAGCTGGAGGCGTTGCAGAAGGCCCTGGAGCCTACAGGCCAGAGCGGAGAGG CAGTGAAGGAGCTTTACTCCCAGCTCGGCGAGAAGCTGGAACAGCTGGATCACCGGAAGCCCAGCCCGGC ACAGGCTGCGGAGACGCCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCCGCTCTGA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_015456 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015456.4, NP_056271.3 |
| RefSeq Size | 2629 bp |
| RefSeq ORF | 1887 bp |
| Locus ID | 25920 |
| UniProt ID | Q8WX92 |
| Protein Families | Transcription Factors |
| Gene Summary | NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213518 | NELFB (Myc-DDK-tagged)-Human cofactor of BRCA1 (COBRA1) |
CNY 4,320.00 |
|
| RC213518L1 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), Myc-DDK-tagged |
CNY 6,720.00 |
|
| RC213518L2 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), mGFP tagged |
CNY 7,030.00 |
|
| RC213518L3 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), Myc-DDK-tagged |
CNY 7,030.00 |
|
| RC213518L4 | Lenti ORF clone of Human cofactor of BRCA1 (COBRA1), mGFP tagged |
CNY 7,030.00 |
|
| RG213518 | NELFB (tGFP-tagged) - Human cofactor of BRCA1 (COBRA1) |
CNY 5,610.00 |
|
| SC114647 | NELFB (untagged)-Human cofactor of BRCA1 (COBRA1) |
CNY 6,400.00 |
