PGAP1 (NM_024989) Human Tagged ORF Clone
CAT#: RG213837
- TrueORF®
PGAP1 (tGFP-tagged) - Human post-GPI attachment to proteins 1 (PGAP1)
ORF Plasmid: DDK
"NM_024989" in other vectors (6)
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CNY 10,640.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Bst1; ISPD3024; MRT42; NEDDSBA; SPG67 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213837 representing NM_024989
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTTCACTCAGTTAATCTCTGGAACCTGGCGTTTTATGTCTTCATGGTCTTTCTGGCAACCCTGG GGCTGTGGGATGTCTTCTTCGGCTTCGAGGAGAATAAGTGCAGTATGAGCTACATGTTTGAGTACCCGGA GTATCAGAAAATAGAACTTCCAAAGAAACTGGCAAAACGCTATCCCGCATATGAGTTGTATCTTTATGGA GAGGGATCCTATGCTGAAGAACACAAAATTCTCCCTTTGACGGGTATTCCAGTTCTCTTTCTTCCTGGTA ATGCTGGAAGTTATAAGCAAGTTCGTTCTATTGGCTCCATTGCACTTAGAAAAGCAGAGGACATTGACTT CAAGTACCACTTTGACTTCTTTAGTGTGAACTTCAATGAAGAACTGGTGGCTTTGTATGGTGGAAGTCTT CAGAAGCAGACCAAGTTTGTACATGAATGTATTAAAACAATTCTCAAACTCTATAAGGGTCAAGAATTTG CTCCAAAAAGTGTGGCAATAATTGGTCATTCTATGGGTGGCCTTGTTGCAAGAGCATTGCTTACACTGAA AAATTTTAAGCATGATCTGATAAATCTTCTTATTACACAAGCCACACCTCATGTTGCTCCTGTGATGCCA TTAGATCGTTTCATTACAGATTTTTATACGACTGTAAACAACTATTGGATTCTAAATGCTCGACACATAA ATTTAACCACACTTTCTGTAGCTGGAGGATTCCGGGATTACCAAGTTCGTTCAGGATTGACTTTTCTACC AAAATTAAGCCATCATACCAGTGCCTTATCTGTTGTGAGTTCAGCAGTGCCTAAGACCTGGGTCTCAACA GACCACCTCTCCATTGTGTGGTGTAAACAATTGCAGTTGACTACAGTTCGAGCATTCTTTGATCTTATTG ATGCTGATACTAAACAAATAACTCAAAATTCCAAGAAGAAACTGTCAGTTTTGTATCACCACTTTATAAG ACACCCATCAAAACATTTTGAGGAAAATCCAGCTATAATTTCTGACTTAACAGGGACATCTATGTGGGTT CTAGTAAAAGTGTCCAAATGGACCTATGTAGCTTACAACGAATCTGAGAAGATATATTTTACATTTCCTC TTGAAAATCATAGAAAAATCTACACTCATGTCTATTGTCAGAGCACTATGCTGGATACAAATAGTTGGAT TTTTGCTTGCATAAACAGCACTTCTATGTGCCTGCAAGGGGTTGATTTATCATGGAAAGCTGAACTGCTG CCAACAATTAAGTATCTGACATTAAGACTTCAAGACTATCCATCTTTGTCTCATCTTGTTGTTTATGTAC CATCTGTTCGTGGAAGTAAGTTTGTTGTAGATTGTGAATTCTTTAAAAAAGAGAAAAGATACATACAGCT TCCTGTAACTCATCTTTTTTCCTTTGGATTGTCTTCAAGGAAAGTGGTGTTAAATACAAATGGCCTATAC TACAATCTAGAGCTTCTGAACTTTGGACAGATATACCAAGCTTTTAAAATCAACGTGGTAAGCAAGTGCT CAGCAGTCAAAGAAGAAATAACCAGTATCTATAGACTTCATATTCCTTGGTCTTATGAAGATTCACTAAC CATTGCACAGGCTCCATCTTCCACAGAAATTTCTCTGAAACTCCATATTGCTCAACCAGAAAACAATACC CATGTGGCATTATTTAAAATGTACACGTCATCAGACTGTCGGTACGAGGTGACAGTTAAGACTTCCTTTT CACAAATACTTGGACAGGTAGTTAGATTTCATGGTGGAGCTCTTCCTGCTTATGTCGTATCTAATATCCT TCTTGCTTATAGAGGACAGTTATATTCTCTTTTCTCAACAGGTTGTTGCTTAGAATATGCTACCATGTTG GATAAAGAAGCCAAACCATACAAAGTTGATCCTTTTGTAATTATCATTAAGTTTCTGTTGGGGTATAAAT GGTTTAAAGAATTGTGGGATGTATTATTGTTACCAGAATTAGATGCCGTCATTTTAACTTGTCAGAGTAT GTGTTTCCCCCTGATATCCTTGATTCTCTTTCTGTTTGGAACGTGTACTGCCTACTGGAGTGGCCTACTG TCTTCTGCATCTGTGAGACTTCTTTCTTCATTGTGGCTAGCTTTGAAAAGACCCTCTGAACTTCCTAAAG ATATCAAGATGATATCACCAGACTTGCCCTTTTTGACAATTGTCTTGATCATAGTTAGTTGGACAACTTG TGGAGCACTAGCCATACTTCTTTCTTATCTTTACTATGTGTTTAAGGTTGTTCATCTGCAAGCCAGCTTA ACAACTTTTAAGAATAGCCAGCCTGTGAATCCCAAACACTCTAGAAGAAGTGAAAAGAAATCCAATCATC ATAAAGACTCCTCAATACACCATCTTCGTTTATCTGCCAACGATGCTGAAGATAGCCTTCGCATGCACAG TACTGTGATTAACTTACTAACATGGATTGTATTACTCAGCATGCCTTCTCTAATTTATTGGCTAAAGAAT CTTAGGTATTATTTTAAACTTAATCCTGATCCATGTAAACCTTTGGCATTTATCCTTATTCCGACTATGG CAATTCTTGGAAATACTTACACTGTTTCAATAAAATCAAGTAAATTGTTGAAGACTACTTCACAATTTCC ACTTCCTCTGGCTGTTGGTGTGATTGCTTTTGGGTCAGCACATTTATATAGGCTTCCATGCTTTGTCTTC ATTCCTCTTTTACTCCATGCATTATGCAACTTTATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213837 representing NM_024989
Red=Cloning site Green=Tags(s) MFLHSVNLWNLAFYVFMVFLATLGLWDVFFGFEENKCSMSYMFEYPEYQKIELPKKLAKRYPAYELYLYG EGSYAEEHKILPLTGIPVLFLPGNAGSYKQVRSIGSIALRKAEDIDFKYHFDFFSVNFNEELVALYGGSL QKQTKFVHECIKTILKLYKGQEFAPKSVAIIGHSMGGLVARALLTLKNFKHDLINLLITQATPHVAPVMP LDRFITDFYTTVNNYWILNARHINLTTLSVAGGFRDYQVRSGLTFLPKLSHHTSALSVVSSAVPKTWVST DHLSIVWCKQLQLTTVRAFFDLIDADTKQITQNSKKKLSVLYHHFIRHPSKHFEENPAIISDLTGTSMWV LVKVSKWTYVAYNESEKIYFTFPLENHRKIYTHVYCQSTMLDTNSWIFACINSTSMCLQGVDLSWKAELL PTIKYLTLRLQDYPSLSHLVVYVPSVRGSKFVVDCEFFKKEKRYIQLPVTHLFSFGLSSRKVVLNTNGLY YNLELLNFGQIYQAFKINVVSKCSAVKEEITSIYRLHIPWSYEDSLTIAQAPSSTEISLKLHIAQPENNT HVALFKMYTSSDCRYEVTVKTSFSQILGQVVRFHGGALPAYVVSNILLAYRGQLYSLFSTGCCLEYATML DKEAKPYKVDPFVIIIKFLLGYKWFKELWDVLLLPELDAVILTCQSMCFPLISLILFLFGTCTAYWSGLL SSASVRLLSSLWLALKRPSELPKDIKMISPDLPFLTIVLIIVSWTTCGALAILLSYLYYVFKVVHLQASL TTFKNSQPVNPKHSRRSEKKSNHHKDSSIHHLRLSANDAEDSLRMHSTVINLLTWIVLLSMPSLIYWLKN LRYYFKLNPDPCKPLAFILIPTMAILGNTYTVSIKSSKLLKTTSQFPLPLAVGVIAFGSAHLYRLPCFVF IPLLLHALCNFM TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024989 |
ORF Size | 2766 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024989.4 |
RefSeq Size | 11113 bp |
RefSeq ORF | 2769 bp |
Locus ID | 80055 |
UniProt ID | Q75T13 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
Gene Summary | The protein encoded by this gene functions early in the glycosylphosphatidylinositol (GPI) biosynthetic pathway, catalyzing the inositol deacylation of GPI. The encoded protein is required for the production of GPI that can attach to proteins, and this may be an important factor in the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. Defects in this gene are a cause an autosomal recessive form of cognitive impairment. [provided by RefSeq, Jul 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213837 | PGAP1 (Myc-DDK-tagged)-Human post-GPI attachment to proteins 1 (PGAP1) |
CNY 6,752.00 |
|
RC213837L1 | Lenti-ORF clone of PGAP1 (Myc-DDK-tagged)-Human post-GPI attachment to proteins 1 (PGAP1) |
CNY 9,152.00 |
|
RC213837L2 | Lenti-ORF clone of PGAP1 (mGFP-tagged)-Human post-GPI attachment to proteins 1 (PGAP1) |
CNY 11,590.00 |
|
RC213837L3 | Lenti-ORF clone of PGAP1 (Myc-DDK-tagged)-Human post-GPI attachment to proteins 1 (PGAP1) |
CNY 11,590.00 |
|
RC213837L4 | Lenti-ORF clone of PGAP1 (mGFP-tagged)-Human post-GPI attachment to proteins 1 (PGAP1) |
CNY 9,152.00 |
|
SC318395 | PGAP1 (untagged)-Human post-GPI attachment to proteins 1 (PGAP1) |
CNY 7,432.00 |