GDF1 (NM_001492) Human Tagged ORF Clone
CAT#: RG214424
- TrueORF®
GDF1 (tGFP-tagged) - Human growth differentiation factor 1 (GDF1)
ORF Plasmid: DDK
"NM_001492" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CERS1; CHTD6; DORV; DTGA3; LAG1; LASS1; RAI; UOG1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214424 representing NM_001492
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCGCCGCAGCAAGGTCCCTGCGGCCACCACCTCCTCCTCCTCCTGGCCCTGCTGCTGCCCTCGC TGCCCCTGACCCGCGCCCCCGTGCCCCCAGGCCCAGCCGCCGCCCTGCTCCAGGCTCTAGGACTGCGCGA TGAGCCCCAGGGTGCCCCCAGGCTCCGGCCGGTTCCCCCGGTCATGTGGCGCCTGTTTCGACGCCGGGAC CCCCAGGAGACCAGGTCTGGCTCGCGGCGGACGTCCCCAGGGGTCACCCTGCAACCGTGCCACGTGGAGG AGCTGGGGGTCGCCGGAAACATCGTGCGCCACATCCCGGACCGCGGTGCGCCCACCCGGGCCTCGGAGCC TGTCTCGGCCGCGGGGCATTGCCCTGAGTGGACAGTCGTCTTCGACCTGTCGGCTGTGGAACCCGCTGAG CGCCCGAGCCGGGCCCGCCTGGAGCTGCGTTTCGCGGCGGCGGCGGCGGCAGCCCCGGAGGGCGGCTGGG AGCTGAGCGTGGCGCAAGCGGGCCAGGGCGCGGGCGCGGACCCCGGGCCGGTGCTGCTCCGCCAGTTGGT GCCCGCCCTGGGGCCGCCAGTGCGCGCGGAGCTGCTGGGCGCCGCTTGGGCTCGCAACGCCTCATGGCCG CGCAGCCTCCGCCTGGCGCTGGCGCTACGCCCCCGGGCCCCTGCCGCCTGCGCGCGCCTGGCCGAGGCCT CGCTGCTGCTGGTGACCCTCGACCCGCGCCTGTGCCACCCCCTGGCCCGGCCGCGGCGCGACGCCGAACC CGTGTTGGGCGGCGGCCCCGGGGGCGCTTGTCGCGCGCGGCGGCTGTACGTGAGCTTCCGCGAGGTGGGC TGGCACCGCTGGGTCATCGCGCCGCGCGGCTTCCTGGCCAACTACTGCCAGGGTCAGTGCGCGCTGCCCG TCGCGCTGTCGGGGTCCGGGGGGCCGCCGGCGCTCAACCACGCTGTGCTGCGCGCGCTCATGCACGCGGC CGCCCCGGGAGCCGCCGACCTGCCCTGCTGCGTGCCCGCGCGCCTGTCGCCCATCTCCGTGCTCTTCTTT GACAACAGCGACAACGTGGTGCTGCGGCAGTATGAGGACATGGTGGTGGACGAGTGCGGCTGCCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214424 representing NM_001492
Red=Cloning site Green=Tags(s) MPPPQQGPCGHHLLLLLALLLPSLPLTRAPVPPGPAAALLQALGLRDEPQGAPRLRPVPPVMWRLFRRRD PQETRSGSRRTSPGVTLQPCHVEELGVAGNIVRHIPDRGAPTRASEPVSAAGHCPEWTVVFDLSAVEPAE RPSRARLELRFAAAAAAAPEGGWELSVAQAGQGAGADPGPVLLRQLVPALGPPVRAELLGAAWARNASWP RSLRLALALRPRAPAACARLAEASLLLVTLDPRLCHPLARPRRDAEPVLGGGPGGACRARRLYVSFREVG WHRWVIAPRGFLANYCQGQCALPVALSGSGGPPALNHAVLRALMHAAAPGAADLPCCVPARLSPISVLFF DNSDNVVLRQYEDMVVDECGCR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001492 |
ORF Size | 1116 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001492.3, NP_001483.2 |
RefSeq Size | 2565 bp |
RefSeq ORF | 1119 bp |
Locus ID | 2657 |
UniProt ID | P27539 |
Protein Families | Druggable Genome, Secreted Protein |
Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. Studies in rodents suggest that this protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. The encoded protein is translated from a bicistronic mRNA that also encodes ceramide synthase 1. Mutations in this gene are associated with several congenital cardiovascular malformations. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214424 | GDF1 (Myc-DDK-tagged)-Human growth differentiation factor 1 (GDF1) |
CNY 3,656.00 |
|
RC214424L3 | Lenti ORF clone of Human growth differentiation factor 1 (GDF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC214424L4 | Lenti ORF clone of Human growth differentiation factor 1 (GDF1), mGFP tagged |
CNY 5,890.00 |
|
SC309037 | GDF1 (untagged)-Human growth differentiation factor 1 (GDF1) |
CNY 3,656.00 |