p95 NBS1 (NBN) (NM_002485) Human Tagged ORF Clone
CAT#: RG214682
- TrueORF®
NBN (tGFP-tagged) - Human nibrin (NBN)
ORF Plasmid: DDK
"NM_002485" in other vectors (6)
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CNY 7,120.00
Cited in 5 publications. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AT-V1; AT-V2; ATV; NBS; NBS1; P95 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214682 representing NM_002485
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAACTGCTGCCCGCCGCGGGCCCGGCAGGAGGAGAACCATACAGACTTTTGACTGGCGTTGAGT ACGTTGTTGGAAGGAAAAACTGTGCCATTCTGATTGAAAATGATCAGTCGATCAGCCGAAATCATGCTGT GTTAACTGCTAACTTTTCTGTAACCAACCTGAGTCAAACAGATGAAATCCCTGTATTGACATTAAAAGAT AATTCTAAGTATGGTACCTTTGTTAATGAGGAAAAAATGCAGAATGGCTTTTCCCGAACTTTGAAGTCGG GGGATGGTATTACTTTTGGAGTGTTTGGAAGTAAATTCAGAATAGAGTATGAGCCTTTGGTTGCATGCTC TTCTTGTTTAGATGTCTCTGGGAAAACTGCTTTAAATCAAGCTATATTGCAACTTGGAGGATTTACTGTA AACAATTGGACAGAAGAATGCACTCACCTTGTCATGGTATCAGTGAAAGTTACCATTAAAACAATATGTG CACTCATTTGTGGACGTCCAATTGTAAAGCCAGAATATTTTACTGAATTCCTGAAAGCAGTTGAGTCCAA GAAGCAGCCTCCACAAATTGAAAGTTTTTACCCACCTCTTGATGAACCATCTATTGGAAGTAAAAATGTT GATCTGTCAGGACGGCAGGAAAGAAAACAAATCTTCAAAGGGAAAACATTTATATTTTTGAATGCCAAAC AGCATAAGAAATTGAGTTCCGCAGTTGTCTTTGGAGGTGGGGAAGCTAGGTTGATAACAGAAGAGAATGA AGAAGAACATAATTTCTTTTTGGCTCCGGGAACGTGTGTTGTTGATACAGGAATAACAAACTCACAGACC TTAATTCCTGACTGTCAGAAGAAATGGATTCAGTCAATAATGGATATGCTCCAAAGGCAAGGTCTTAGAC CTATTCCTGAAGCAGAAATTGGATTGGCGGTGATTTTCATGACTACAAAGAATTACTGTGATCCTCAGGG CCATCCCAGTACAGGATTAAAGACAACAACTCCAGGACCAAGCCTTTCACAAGGCGTGTCAGTTGATGAA AAACTAATGCCAAGCGCCCCAGTGAACACTACAACATACGTAGCTGACACAGAATCAGAGCAAGCAGATA CATGGGATTTGAGTGAAAGGCCAAAAGAAATCAAAGTCTCCAAAATGGAACAAAAATTCAGAATGCTTTC ACAAGATGCACCCACTGTAAAGGAGTCCTGCAAAACAAGCTCTAATAATAATAGTATGGTATCAAATACT TTGGCTAAGATGAGAATCCCAAACTATCAGCTTTCACCAACTAAATTGCCAAGTATAAATAAAAGTAAAG ATAGGGCTTCTCAGCAGCAGCAGACCAACTCCATCAGAAACTACTTTCAGCCGTCTACCAAAAAAAGGGA AAGGGATGAAGAAAATCAAGAAATGTCTTCATGCAAATCAGCAAGAATAGAAACGTCTTGTTCTCTTTTA GAACAAACACAACCTGCTACACCCTCATTGTGGAAAAATAAGGAGCAGCATCTATCTGAGAATGAGCCTG TGGACACAAACTCAGACAATAACTTATTTACAGATACAGATTTAAAATCTATTGTGAAAAATTCTGCCAG TAAATCTCATGCTGCAGAAAAGCTAAGATCAAATAAAAAAAGGGAAATGGATGATGTGGCCATAGAAGAT GAAGTATTGGAACAGTTATTCAAGGACACAAAACCAGAGTTAGAAATTGATGTGAAAGTTCAAAAACAGG AGGAAGATGTCAATGTTAGAAAAAGGCCAAGGATGGATATAGAAACAAATGACACTTTCAGTGATGAAGC AGTACCAGAAAGTAGCAAAATATCTCAAGAAAATGAAATTGGGAAGAAACGTGAACTCAAGGAAGACTCA CTATGGTCAGCTAAAGAAATATCTAACAATGACAAACTTCAGGATGATAGTGAGATGCTTCCAAAAAAGC TGTTATTGACTGAATTTAGATCACTGGTGATTAAAAACTCTACTTCCAGAAATCCATCTGGCATAAATGA TGATTATGGTCAACTAAAAAATTTCAAGAAATTCAAAAAGGTCACATATCCTGGAGCAGGAAAACTTCCA CACATCATTGGAGGATCAGATCTAATAGCTCATCATGCTCGAAAGAATACAGAACTAGAAGAGTGGCTAA GGCAGGAAATGGAGGTACAAAATCAACATGCAAAAGAAGAGTCTCTTGCTGATGATCTTTTTAGATACAA TCCTTATTTAAAAAGGAGAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214682 representing NM_002485
Red=Cloning site Green=Tags(s) MWKLLPAAGPAGGEPYRLLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKD NSKYGTFVNEEKMQNGFSRTLKSGDGITFGVFGSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTV NNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQPPQIESFYPPLDEPSIGSKNV DLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTCVVDTGITNSQT LIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDPQGHPSTGLKTTTPGPSLSQGVSVDE KLMPSAPVNTTTYVADTESEQADTWDLSERPKEIKVSKMEQKFRMLSQDAPTVKESCKTSSNNNSMVSNT LAKMRIPNYQLSPTKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLL EQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIED EVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDS LWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLP HIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002485 |
ORF Size | 2262 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002485.5 |
RefSeq Size | 4639 bp |
RefSeq ORF | 2265 bp |
Locus ID | 4683 |
UniProt ID | O60934 |
Domains | FHA |
Protein Families | Druggable Genome |
Protein Pathways | Homologous recombination |
Gene Summary | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
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DNA damage causes rapid accumulation of phosphoinositides for ATR signaling
,Wang, YH;Hariharan, A;Bastianello, G;Toyama, Y;Shivashankar, GV;Foiani, M;Sheetz, MP;,
Nat Commun
,PubMed ID 29242514
[NBN]
|
Localized Movement and Levels of 53BP1 Protein Are Changed by γ-irradiation in PML Deficient Cells.
,null,
Journal of cellular biochemistry
,PubMed ID 27526954
[NBN]
|
Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle-dependent formation of γH2AX- and NBS1-positive repair foci.
,null,
Biology of the cell
,PubMed ID 26482424
[NBN]
|
Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction: DNA Repair and Epigenetics of Ribosomal Genes
,Franek, M;Kovaríková, A;Bártová, E;Kozubek, S;,
J. Histochem. Cytochem.
,PubMed ID 27680669
[NBN]
|
Localized Movement and Levels of 53BP1 Protein Are Changed by ?-Irradiation in PML Deficient Cells
,Legartová, S;Sehnalová, P;Malyšková, B;,
Journal of Cellular Biochemistry
[NBN]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214682 | NBN (Myc-DDK-tagged)-Human nibrin (NBN) |
CNY 5,520.00 |
|
RC214682L1 | Lenti ORF clone of Human nibrin (NBN), Myc-DDK-tagged |
CNY 7,920.00 |
|
RC214682L2 | Lenti ORF clone of Human nibrin (NBN), mGFP tagged |
CNY 5,890.00 |
|
RC214682L3 | Lenti ORF clone of Human nibrin (NBN), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC214682L4 | Lenti ORF clone of Human nibrin (NBN), mGFP tagged |
CNY 7,920.00 |
|
SC118613 | NBN (untagged)-Human nibrin (NBN) |
CNY 8,296.00 |
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