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C14orf151 (INF2) (NM_001031714) Human Tagged ORF Clone
CAT＃: RG215188
- TrueORF®
INF2 (tGFP-tagged) - Human inverted formin, FH2 and WH2 domain containing (INF2), transcript variant 2
ORF Plasmid: DDK
"NM_001031714" in other vectors (2)
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CNY 13,590.00
货期*
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规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C14orf151; C14orf173; CMTDIE; FSGS5; pp9484 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215188 representing NM_001031714
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGAAGGAGGGCGCACAGCGCAAGTGGGCAGCGCTGAAGGAGAAGCTGGGGCCACAGGATTCGG ACCCCACGGAGGCCAACCTGGAGAGCGCGGACCCCGAGCTGTGCATCCGGCTGCTCCAGATGCCCTCTGT GGTCAACTACTCCGGCCTGCGCAAGCGCCTGGAGGGCAGCGACGGCGGCTGGATGGTGCAGTTCCTGGAG CAGAGCGGCCTGGACCTGCTGCTGGAGGCGCTGGCGCGGCTGTCGGGCCGCGGCGTTGCACGTATCTCCG ACGCCCTGCTGCAGCTCACCTGCGTCAGCTGCGTGCGCGCCGTCATGAACTCGCGGCAGGGCATCGAGTA CATCCTCAGCAACCAGGGCTACGTGCGCCAGCTCTCCCAGGCCCTGGACACATCCAACGTGATGGTGAAG AAGCAGGTGTTTGAGCTACTGGCTGCCCTGTGCATCTACTCTCCCGAGGGCCACGTGCTGACCCTGGACG CCCTGGACCACTACAAGACGGTGTGCAGCCAGCAGTACCGCTTCAGCATTGTCATGAACGAGCTCTCCGG CAGCGACAACGTGCCCTACGTGGTCACCCTGCTTAGCGTGATCAACGCCGTCATCTTGGGCCCCGAGGAC CTGCGCGCGCGCACCCAGCTGCGGAACGAGTTTATCGGGCTGCAGCTGCTGGACGTCCTGGCTCGCCTGC GAGACCTGGAGGATGCCGACCTGCTGATCCAGCTGGAGGCTTTCGAGGAGGCTAAGGCCGAGGACGAGGA GGAGCTGCTGCGAGTCTCTGGCGGGGTCGACATGAGCAGCCACCAGGAGGTCTTTGCCTCCCTGTTCCAC AAGGTGAGCTGCTCCCCGGTGTCTGCCCAGCTCCTGTCGGTGCTGCAGGGCCTCCTGCACCTGGAGCCCA CCCTCCGCTCCAGCCAGCTGCTCTGGGAGGCCCTGGAGAGCCTCGTGAACCGGGCCGTGCTCCTGGCCAG CGATGCCCAGGAATGCACCCTGGAGGAAGTGGTTGAGCGGCTCCTGTCTGTCAAGGGGCGACCCAGACCG AGCCCCCTGGTCAAGGCCCATAAAAGCGTCCAGGCCAACCTAGACCAGAGCCAGAGGGGCAGCTCCCCGC AAAACACTACAACCCCCAAGCCCAGCGTGGAGGGCCAGCAGCCAGCAGCAGCTGCTGCCTGCGAGCCCGT GGACCACGCCCAGAGTGAGAGCATCCTGAAAGTTTCGCAGCCCAGAGCCCTGGAGCAGCAGGCGTCCACC CCACCCCCACCCCCACCCCCACCCCTGCTCCCTGGTTCCAGTGCCGAGCCCCCTCCCCCTCCCCCACCAC CCCCCCTGCCCAGTGTGGGGGCTAAGGCCCTCCCAACAGCACCCCCGCCCCCACCCCTGCCAGGCCTGGG GGCCATGGCCCCCCCAGCACCTCCTCTACCACCACCCCTGCCAGGCTCCTGTGAGTTCCTGCCCCCACCA CCTCCACCACTCCCGGGCTTGGGATGCCCGCCCCCACCCCCACCCCTGCTGCCTGGTATGGGCTGGGGCC CTCCTCCACCCCCACCTCCACTACTGCCCTGCACCTGCAGCCCCCCCGTGGCGGGAGGCATGGAGGAGGT CATCGTGGCCCAGGTGGACCATGGCTTGGGCTCAGCATGGGTCCCCAGCCATCGGCGGGTGAACCCACCC ACACTGCGCATGAAGAAGCTGAACTGGCAGAAGCTGCCATCCAACGTGGCACGTGAGCACAACTCTATGT GGGCGTCCCTGAGCAGCCCCGACGCCGAGGCTGTGGAGCCCGACTTCTCCAGCATCGAGCGACTATTCTC CTTCCCTGCAGCCAAGCCCAAGGAGCCCACCATGGTGGCCCCCCGGGCCAGGAAGGAGCCCAAGGAGATC ACTTTCCTCGATGCCAAGAAGAGCCTGAACCTCAACATCTTCCTGAAGCAATTTAAGTGCTCCAACGAGG AGGTCGCTGCTATGATCCGGGCTGGAGATACCACCAAGTTTGATGTGGAGGTTCTCAAACAACTCCTTAA GCTCCTTCCCGAGAAGCACGAGATTGAAAACCTGCGGGCATTCACAGAGGAGCGAGCCAAGCTGGCCAGC GCCGACCACTTCTACCTCCTCCTGCTGGCCATTCCCTGCTACCAGCTGCGAATCGAGTGCATGCTGCTGT GTGAGGGCGCGGCCGCCGTGCTGGACATGGTGCGGCCCAAGGCCCAGCTGGTGCTGGCTGCCTGCGAAAG CCTGCTCACCAGCCGCCAGCTGCCCATCTTCTGCCAGCTGATCCTGAGAATTGGGAACTTCCTCAACTAC GGCAGCCACACCGGTGACGCCGACGGCTTCAAGATCAGCACATTGCTGAAGCTCACGGAGACCAAGTCCC AGCAGAACCGCGTGACGCTGCTGCACCACGTGCTGGAGGAAGCGGAAAAGAGCCACCCCGACCTCCTGCA GCTGCCCCGGGACCTGGAACAGCCCTCGCAAGCAGCAGGGATCAACCTGGAGATCATCCGCTCAGAGGCC AGCTCCAACCTGAAGAAGCTTCTGGAGACCGAGCGGAAGGTGTCTGCCTCCGTGGCCGAGGTCCAGGAGC AGTACACCGAGCGCCTCCAGGCCAGCATCTCGGCCTTCCGGGCACTGGATGAGCTGTTTGAGGCCATCGA GCAGAAGCAACGGGAGCTGGCCGACTACCTGTGTGAGGACGCCCAGCAGCTGTCCCTGGAGGACACGTTC AGCACCATGAAGGCTTTCCGGGACCTTTTCCTCCGCGCCCTGAAGGAGAACAAGGACCGGAAGGAGCAGG CGGCGAAGGCAGAGAGGAGGAAGCAGCAGCTGGCGGAGGAGGAGGCGCGGCGGCCTCGGGGAGAGGACGG GAAGCCTGTCAGGAAGGGGCCCGGGAAGCAGGAGGAGGTGTGTGTCATCGATGCCCTGCTGGCTGACATC AGGAAGGGCTTCCAGCTGCGGAAGACAGCCCGGGGCCGCGGGGACACCGACGGGGGCAGCAAGGCAGCCT CCATGGATCCCCCAAGAGCCACAGAGCCTGTGGCCACCAGTAACCCTGCAGGAGATCCCGTGGGCAGCAC GCGCTGTCCCGCCTCTGAGCCCGGCCTTGATGCTACAACAGCCAGCGAGTCCCGGGGCTGGGACCTTGTA GACGCCGTGACCCCCGGCCCTCAGCCCACCCTGGAGCAGTTGGAGGAGGGTGGTCCACGGCCCCTGGAGA GGCGTTCTTCCTGGTATGTGGATGCCAGCGATGTCCTAACCACTGAGGATCCCCAGTGCCCCCAGCCCTT GGAGGGGGCCTGGCCGGTGACTCTGGGAGATGCTCAGGCCCTGAAGCCCCTCAAGTTCTCCAGCAACCAG CCCCCTGCAGCCGGAAGTTCAAGGCAAGATGCCAAGGATCCCACGTCCTTGCTGGGCGTCCTCCAGGCCG AGGCCGACAGCACAAGTGAGGGGCTGGAGGACGCTGTCCACAGCCGTGGTGCCAGACCCCCTGCAGCAGG CCCAGGTGGGGATGAGGACGAGGACGAGGAGGACACGGCCCCAGAGTCCGCACTGGACACATCCCTGGAC AAGTCCTTCTCCGAGGATGCGGTGACCGACTCCTCGGGGTCGGGCACACTCCCCAGGGCCCGGGGCCGGG CCTCAAAGGGGACCGGGAAGCGAAGGAAGAAGCGTCCCTCCAGGAGCCAGGAAGGCCTCAGGCCCAGGCC CAAGGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215188 representing NM_001031714
Red=Cloning site Green=Tags(s) MSVKEGAQRKWAALKEKLGPQDSDPTEANLESADPELCIRLLQMPSVVNYSGLRKRLEGSDGGWMVQFLE QSGLDLLLEALARLSGRGVARISDALLQLTCVSCVRAVMNSRQGIEYILSNQGYVRQLSQALDTSNVMVK KQVFELLAALCIYSPEGHVLTLDALDHYKTVCSQQYRFSIVMNELSGSDNVPYVVTLLSVINAVILGPED LRARTQLRNEFIGLQLLDVLARLRDLEDADLLIQLEAFEEAKAEDEEELLRVSGGVDMSSHQEVFASLFH KVSCSPVSAQLLSVLQGLLHLEPTLRSSQLLWEALESLVNRAVLLASDAQECTLEEVVERLLSVKGRPRP SPLVKAHKSVQANLDQSQRGSSPQNTTTPKPSVEGQQPAAAAACEPVDHAQSESILKVSQPRALEQQAST PPPPPPPPLLPGSSAEPPPPPPPPPLPSVGAKALPTAPPPPPLPGLGAMAPPAPPLPPPLPGSCEFLPPP PPPLPGLGCPPPPPPLLPGMGWGPPPPPPPLLPCTCSPPVAGGMEEVIVAQVDHGLGSAWVPSHRRVNPP TLRMKKLNWQKLPSNVAREHNSMWASLSSPDAEAVEPDFSSIERLFSFPAAKPKEPTMVAPRARKEPKEI TFLDAKKSLNLNIFLKQFKCSNEEVAAMIRAGDTTKFDVEVLKQLLKLLPEKHEIENLRAFTEERAKLAS ADHFYLLLLAIPCYQLRIECMLLCEGAAAVLDMVRPKAQLVLAACESLLTSRQLPIFCQLILRIGNFLNY GSHTGDADGFKISTLLKLTETKSQQNRVTLLHHVLEEAEKSHPDLLQLPRDLEQPSQAAGINLEIIRSEA SSNLKKLLETERKVSASVAEVQEQYTERLQASISAFRALDELFEAIEQKQRELADYLCEDAQQLSLEDTF STMKAFRDLFLRALKENKDRKEQAAKAERRKQQLAEEEARRPRGEDGKPVRKGPGKQEEVCVIDALLADI RKGFQLRKTARGRGDTDGGSKAASMDPPRATEPVATSNPAGDPVGSTRCPASEPGLDATTASESRGWDLV DAVTPGPQPTLEQLEEGGPRPLERRSSWYVDASDVLTTEDPQCPQPLEGAWPVTLGDAQALKPLKFSSNQ PPAAGSSRQDAKDPTSLLGVLQAEADSTSEGLEDAVHSRGARPPAAGPGGDEDEDEEDTAPESALDTSLD KSFSEDAVTDSSGSGTLPRARGRASKGTGKRRKKRPSRSQEGLRPRPKAK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031714 |
| ORF Size | 3720 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001031714.4 |
| RefSeq Size | 4668 bp |
| RefSeq ORF | 3723 bp |
| Locus ID | 64423 |
| UniProt ID | Q27J81 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.[provided by RefSeq, Aug 2010] |
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